摘要:

SUMMARYThe presence of intercellular adhesion molecule‐1 (ICAM‐1) on keratinocytes of psoriatic skin lesions before and during 8‐methoxapsoralen and UVA light (PUVA) treatment was studied. ICAM‐1 was expressed on the keratinocytes in biopsies of the skin lesions of five patients with psoriasis. The patients who responded to PUVA treatment had a concurrent reduction of ICAM‐1 expression on the keratinocytes with a reduction of the number of cells in the mononuclear cellular infiltrate and a lessening of the severity of the disease. Patients who went into remission during therapy and then relapsed showed an increase in ICAM‐1 expression on keratinocytes with an increase in the number of cells in the mononuclear cell infiltrate and an increase in the severity of the disease. HLA‐DR expression on keratinocytes was variable during treatment and showed no strong correlation with dis

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01320.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYLangerhans cell histiocytosis (LCH), or histiocytosis X, is now generally considered to be a non‐malignant condition. A flow cytometric (FCM) study of a single case has, however, been published which claimed to provide evidence to contradict this. The presence of DNA‐ploidy as detected using this technique is a feature of malignant and pre‐malignant disease. In this reported single case, DNA‐ploidy was present but the clinical features of this patient were atypical for LCH. We have performed a FCM study of the DNA of nine biopsies of LCH lesions from six patients with well‐established disease. In addition, in one of these, fresh tissue studies including the use of an anti‐CD1 monoclonal antibody to specifically label the LCH cells were performed. In all cases the DNA content of the cells was entirely normal. We therefore found no evidence that LCH is a neoplast

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01321.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe effects of local hyperthermia treatment on contact sensitivity (CS) and on the number of Langerhans cells (LCs) were studied in mice. CS was significantly suppressed when mice were sensitized in the hyperthermia treated skin 1, 2 or 4 days after treatment (43°C for 45 min). This suppressive effect was not observed 7 or 14 days after the treatment. CS was also suppressed when mice were sensitized in non‐treated skin 1 day after the treatment. The density of LCs detected as ATPase‐positive cells also decreased significantly 1, 2, 4 and 7 days after the treatment. There appeared to be a positive correlation between the number of LCs and the extent of CS when mice were sensitized at hyperthermia treated skin. It was observed that this suppressive effect on CS was dose‐ and temperature‐dependent. It could be transferred by spleen cells from the hyperthermia treated and DNFB‐sensitized donors, and was antigen specific when spleen cells were transferred before sensitization of the recipient mice. This indicated it was, in part, associated with the induction of suppressor cells. These findings suggest that local hyperthermia treatment reduces the number of LCs with subsequent suppression of the induction phase of delayed‐type hypersensitivity by the genertion of antigen‐specific su

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01322.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYScaly skin occurs in 80‐90% of patients who are hypothyroid, the pathogenesis of which is unknown. Since thyroid hormone (T3) affects growth and differentiation in other organs, we examined the effects of its absence on keratinocytesin vitro.Human neonatal foreskin keratinocytes were cultivated and second passage cells were switched to T3‐depleted (–T3) medium at 50% confluence. Cells maintained in the –T3 medium demonstrated increased (1.5 fold) levels of the cross‐linking enzyme transglutaminase and increased (1.5 fold) formation of cornified envelopes, when compared to keratinocytes maintained in medium containing physiologic levels (2 × 10−9M) of T3. Additionally, in the –T3 cultures, the level of the protease plasminogen activator (PA), an enzyme implicated in the process of shedding of cornified cells, was decreased 70‐80% of that measured in +T3 media.Absence of T3 from keratinocyte culture‐medium increased both the level of the enzyme responsible for cross‐linking cornified envelope precursors and the rate of envelope formation in cultured cells. The decreased levels of PA observed in the –T3 cultures could result in decreased shedding of cornified cells. These alterations in the process of keratinocyte differentiation may explain the clinically observed scaliness associated with hypothyroidism in humans. The molecular mechanism by which T3 alters keratinocyte cornific

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01323.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYOne hundred and six melanocytic lesions were studied to determine the value of the so called Ag NOR technique in differentiating dysplastic naevi, Spitz naevi and spindle cell naevi of Reed from malignant melanoma and from ‘banal’ compound or intradermal naevi. In 79 cases Ag NOR counts were possible. The banal naevi (36) had a mean count of 1.54, (SD 0.3), and the unequivocally malignant superficial spreading melanomas (13), lentigo maligna melanomas (4) and secondary melanomas (4) had a mean count overall of 3.9 (SD 1.59). For dysplastic naevi (16) the mean Ag NOR count was 1.63 (SD 0.36) and for Spitz and spindle cell naevi (in toto6) the figure was 1.72 (SD 0.55). The difference in Ag NOR counts between all types of naevi and all types of melanoma was highly statistically significant, but there was no difference between banal naevi and dysplastic, Spitz, and spindle cell naevi. Correlation of Ag NOR counts between three independent observers was good. This technique may, therefore, be a useful adjunct in separating true melanoma from borderline melanocytic na

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01324.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe minimal erythema doses (MED) to UVB and UVA radiation were measured in 254 normal subjects. Bivariate correlation analysis applied to the data showed a strong positive correlation between the UVB and UVA MEDs. By calculating the probability that a given combination of UVB and UVA MEDs is likely to occur in normal subjects, it was shown that, in some instances, both the UVB MEDs and UVA MED observed in a given subject may be within their respective normal ranges, but that the particular combination is more in keeping with abnormal photosensitivity.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01325.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe effects were studied of two antipsoriatic drugs, dithranol (anthralin) and butantrone on the proliferation of HeLa cells. Non‐confluent monolayers of HeLa cells were treated with various concentrations (10−5‐10−8M) of dithranol or butantrone for 45 min. The cells were then cultivated for up to 48 h and treated with 10 μM bromodeoxyuridine (BrdU) and 10 μM fluorodeoxyuridine for 30 min before harvesting. Both drugs at concentrations of 10−6and 10−5M inhibited the BrdU uptake of HeLa cells, but did not cause any significant change in the proportion of cells in S‐phase. The inhibitory effect of dithranol on cell number was linear with concentration 24 h after incubation. The highest (10−5M) concentration of butantrone caused an abrupt decrea

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01326.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe sera from two patients with epidermolysis bullosa acquisita were blotted against dermal extracts in comparison with the mouse monoclonal antibody LH 7.2. This antibody reacts with carboxy terminal region of type VII collagen. The epidermolysis bullosa acquisita antisera showed binding to the same molecular weight protein as LH 7.2 confirming that the target antigen for epidermolysis bullosa acquisita antibodies is the carboxy terminal region of type VII collagen. This newly described collagen forms the major component of anchoring fibrils. These findings are consistent with established ultrastructural data which have shown that the epidermolysis bullosa acquisita antigen is located within and below the lamina densa. The monoclonal antibody LH 7.2 provides an internal standard for epidermolysis bullosa acquisita autoantisera activity. The use of immunoblotting of epidermolysis bullosa autoantisera in comparison with the monoclonal antibody LH 7.2 provides definitive investigation for the diagnosis of this disorder.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01327.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe histological features of lichen planus (LP) are characterized by typical epidermal change with dermal lymphocytes that are mostly Ia positive T cells. In order to find out whether the primary event of LP is damage to basal keratinocytes or a delayed hypersensitivity reaction in which an as yet unidentified antigen activates T lymphocytes that destroy keratinocytes, we transplanted skin obtained from six patients with LP. Two millimetre punch and split thickness of grafts were obtained from involved and uninvolved areas from each patient and grafted onto nude mice. Biopsies were taken from the grafts at 14 and 21 days after transplantation for histological and immunofluorescence studies and after 6 weeks for Dopa incubation for melanocyte populations. A complete disappearance of the pathological changes of LP was found 21 days following grafting. An increased number of melanocytes was noted. This indicates that the pathogenesis of LP may not be due to an inherent change in the epidermal cells, but rather to the migration of cellular elements of the immune system.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01328.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYIn an attempt to substantiate the claim that pityriasis rotunda may be a useful cutaneous marker of hepatocellular carcinoma in South African Blacks, the prevalence of the rash in 63 unselected South African Blacks with this tumour was compared to that in 63 matched patients with active tuberculosis, 63 with other malignant tumours, and 63 with various forms of chronic benign hepatic disease. The prevalence of pityriasis rotunda in hepatocellular carcinoma was 15.9%, which was appreciably greater (P=0.0005) than the overall prevalence of the rash (2.6%) in the controls. The prevalence was 4.8% for tuberculosis (P=0.038), 0% for other malignant diseases (P= 0.0007), and 3.2% for chronic benign hepatic disease (P= 0.015). We conclude that the presence of pityriasis rotunda is a useful pointer to the diagnosis of hepatocellular carcinoma in South African Blacks.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01329.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY