摘要:

SummaryThis article is a review of the role of the endothelium in psoriasis, with emphasis on angiogenesis and lymphocyte—endothelial interaction

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb08702.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryPrimary cutaneous medium and large cell lymphomas (MLCL) other than mycosis fungoides (MF) are rare, and their prognosis and treatment are controversial. The clinical, immunohistological and follow‐up data of 54 well‐documented cases of primary cutaneous MLCL other than MF, seen in our institutions over a 14‐year period, were retrospectively reviewed, in order to determine the prognostic factors related to these lymphomas, and to analyse the results obtained with different treatment regimens. Forty‐six patients presented with a solitary tumour or with localized lesions. and eight had disseminated cutaneous lesions. According to the updated Kiel classification, 45 cases (83%) corresponded to B‐cell lymphomas: centroblastic lymphomas, 32 cases; centroblastic‐centrocytic lymphomas, 11 cases; immunoblastic lymphomas, two cases. Nine cases (17%) were classified as T‐cell lymphomas: pleomorphic medium and large cell lymphomas, eight cases; anaplastic large cell lymphoma. one case. Four of eight patients with disseminated skin lesions had a T‐cell lymphoma. whereas 41 of 46 patients with a solitary tumour had a B‐cell lymphoma. Patients with disseminated skin lesions and elevated serum lactate dehydrogenase (LDH) levels had a poor prognosis. Comparison of patients' overall survival, depending on immunohistological subtype, showed that the median survival of patients with pleomorphic T‐cell lymphoma was 2·5 years, whereas it was not reached at 12 years for patients with centroblastic centrocytic and centroblastic lymphoma. The eight patients with disseminated skin lesions were treated with polychemotherapy. Most patients with a solitary tumour or with localized lesions of low tumour bulk were treated by surgical excision or radiotherapy alone, and nine other patients with localized lesions of high tumour bulk were treated with initial polychemotherapy. Clinical presentation (i.e. solitary or disseminated lesions), serum LDH levels, and the immunohistological subtype, are important prognostic factors in cutaneous MLCL. Patients with disseminated skin lesions have a poor prognosis, and should be treated with intensive polychemotherapy regimens, whereas those with a solitary tumour, or with localized lesions of low tumour bulk, are adequately tre

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb08703.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryThe p53 protein is the product of a tumour suppressor gene, which is implicated in many human malignancies. p53 expression was investigated by immunohistochemistry in a series of viral warts (n = 12) from five patients with epidermodysplasia verruciformis (EV), using a monoclonal anti‐p53 antibody (D07). p53 expression was also investigated in a series of common warts (n = 8), flat warts (n= 8), and penile bowenoid papulosis (n = 6) from non‐EV patients. Immunostaining was positive in 11 of 12 (92%) EV warts, whereas p53 reactivity was negative in most cases of warts from non‐EV patients. Exons 5–8 of the p53 gene were screened by the polymerase chain reaction‐single strand conformation polymorphism technique in four EV warts, which were strongly stained for p53, and p53 mutations were not detected. These results suggest an association between p53 accumulation (probably of wild type) and

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb08704.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryNeonatal lupus erythematosus (NLE) is an antibody‐mediated disorder most commonly associated with autoantibodies to Ro and/or La antigens. There have been five previous reports describing eight NLE patients with anti‐U1RNP antibody in the absence of anti‐Ro and anti‐La autoantibodies. We report two cases of anti‐U1RNP antibody‐positive NLE, and briefly review the five previous reports. The diagnosis of NLE was based on physical examination and serotogical studies by ELISA, immunodiffusion, and immunoblotting. By conventional immunodiffusion and ELISA, our cases were negative for anti‐Ro and anti‐La antibodies, and positive for anti‐U1RNP antibody. However, one of the mothers had anti‐La antibody detected by immunoblot assay only. All anti‐U1RNP antibody‐positive infants had classic cutaneous lesions of NLE, but it is of interest that none had congenital heart block (CHB). Although these infants were negative for anti‐Ro and anti‐La antibodies with immunodiffusion and EL ISA techniques, these antibodies might be detectable by immunoblotting, as was the

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb08705.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryTo investigate the mode of action of testosterone on human hair follicles, we studied the localization of androgen receptors in hair follicles and cultured dermal papilla cells from different body sites, using immunohistochemistry with a polyclona) antibody. Androgen receptors were detected in the dermal papilla cells of beard and axillary hair follicles, but not in those of occipital scalp hair follicles. Epithelial cells of the hair follicles were not stained by the antibody. Androgen receptors were also detected in the nuclei of cultured beard and axillary dermal papilla cells, but the dermal papilla cells from occipital scalp hair follicles showed little staining with this antibody. We also examined the effects of testosterone on the DNA synthesis and proliferation of cultured outer root sheath cells and dermal papilla cells. Testosterone did not have a proliferative effect on either type of cell when cultured alone. In contrast, testosterone significantly stimulated the proliferation of outer root sheath cells which were co‐cultured with beard or axillary dermal papilla cells, without cell contact. No such effect was observed when dermal papilla cells from occipital scalp hair follicles were used in the cultures. This proliferative effect of testosterone was concentration‐dependent, and was antagonized by cyproterone acetate. Our findings suggest that dermal papilla cells from the beard and axillary hair follicles produce androgen‐dependent diffusible growth factors which stimulate follicular epithelial

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb08706.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryAlthough epidermoid cysts of the palms and soles have long been assumed to develop following implantation of an epidermal fragment as a result of a penetrating injury, the pathogenic mechanism is still controversial, and the discovery of a more common aetiological agent is awaited. Clinical, histological, immunohistochemical and molecular biological studies were performed on 119 epidermoid cysts of palmoplatitur location, in order to examine the rote of the eccrine ducts, and human papillomavirus (HPV), in the pathogenesis of this disorder.Characteristic histological features were found, including intracytoplasmic eosinophilic bodies (ICB: in 14 cases. 12%) in the cyst wall, vacuolar structures (V: in 28 cases, 24%), or parakeratotic nuclei (P: in 85 cases. 71%) within the keratinous mass in the cyst cavity. Ductal structures suggesting eccrine ducts (E: in 63 cases, 53%) were also found in the cyst wall or in the cyst cavity. Hither ductal structures or carcinoembryonic antigen expression (66 cases, 55%) were noted in a total of 73 cases (61%). Papillomavirus common antigens were detected in 36 cases (30%) showing one or more of the three distinct histological features, i.e. ICB, V and P, Subsequently, hybridization experiments to detect HPV DNA were performed in 47 cases, revealing an association between cysts showing ICB or V and the presence of HPV 60 DNA sequences.On the basis of our results, we propose that epidermoid cysts in the palmoplantar regions may develop from eccrine ducts, and that HPV and injury may play a role in their pathogenesis.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb08707.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Summary Eighty‐nine patients with chronic actinic dermatitis (CAD) were retrospectively studied: 69 (78%) male and 20 (22%) female, with mean ages of 66 and 64 years, respectively; nine (10%) were dark skinned. Eight (9%) were abnormally sensitive to UVB wavelengths alone, 74 (83%) to UVB and UVA, and seven (8%) to UVB, UVA and visible radiation. Eighty‐six patients were patch tested to an extended standard European series of contact allergens, including in 80 cases a 0.1% mix of three sesquiterpene lactones, and photopatch tested to a standard photopatch series. Sixty‐four of these 86 patients (74%) had positive patch or photopatch tests; 36% (29 of 80) were sensitive to the sesquiterpene lactone mix, 21% (18 of 86) to fragrance compounds, 20% (17 of 86) to colophony, and 14% (12 of 86) to rubber chemicals. Ten (12%) had positive photopatch tests; five (6%) to musk ambrette, six (7%) to sunscreens and one to both. Fourteen of the eighty‐nine patients with CAD (16%) had preceding endogenous eczema. In 18 of 86 patients (21%), CAD occurred alone, with neither detectable contact nor photocontact allergy, nor a preceding history of endogenous eczema. This study confirms the association between Compositae (sesquiterpene lactone) dermatitis

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb08708.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryFlow cytometric DNA analysis of various tumours has indicated a correlation between the degree of malignancy and ploidy; results which could have clinical significance. We analysed the ploidy of Kaposi's sarcoma (KS) tumours, and classified the results according to clinical history and histological findings. We found that patients on steroid treatment had an aneuploid pattern, and most of the patients with classic‐type KS had a diploid pattern on flow cytometr

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb08709.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryMetastatic malignant melanoma is notoriously resistant to chemotherapeutic agents, but the exact mechanisms involved in this drug resistance arc unknown. One recently defined major mechanism of multidrug resistance involves the overexpression of P‐glycoprotein on cell membranes. In order to evaluate the significance of this putative drug efflux pump for chemoresistance of malignant melanoma, five different antibodies were employed to examine P‐glycoprotein expression on tissue from 33 primary malignant melanomas and 35 metastases, before and after chemotherapy, using immunohistological techniques. The expression of P‐glycoprotein was low on primary cutaneous melanomas (three of 33), and on metastases (one of 35). Normal tissue in and around the melanoma showed reactivity of endothelial cells, stromal cells and eccrine sweat glands with several antibodies tested. Chemotherapy with drugs commonly used in metastatic melanoma, including agents known to induce P‐glycoprotein expression in other tumours (vindesine, cisplatin) had no effect on P‐glycoprotein expression in human melanoma metastases. The high chemoresistance of human melanoma cells in vitro and in vivo is probably not mediated via P‐glycoprotein, and other possible mechanisms involved will have to be explored in fut

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb08710.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryMetastatic melanoma was staged in l5 patients using whole‐body positron emission tomography (PET) and the radiopharmaceutical 2‐fluorine‐18‐fluoro‐2‐deoxy‐D‐glucose (FDG). PET correctly demonstrated 30 metastases in lung, brain, pancreas, nasal cavity, skin and subcutaneous tissue, and lymph nodes. It detected 97% of all metastases exceeding its spatial resolution (>5mm). Two cutaneous metastases (approximately 3 mm) did not show increased FDG uptake; the overall detection sensitivity was 91%. Two false‐positive lesions in one patient were due to severe wound infection. PET correctly excluded malignancy in four cases where suspicious lesions were found with conventional cross‐sectional imaging modalities but later ruled out by fine‐needle biopsy.PET therefore proved to be an excellent method for staging of metastatic melanoma. Due to its high sensitivity for malignant lesions and the possibility of covering the whole body in one examination, it can replace staging techniques employing multiple imaging modalities: chest X‐ray, ultrasonography and computed tomography. Furthermore, it provides information on the malignant potential

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb08711.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY