摘要:

SummaryKeratinocyte differentiation in psoriasis was examined using a panel of monospecific monoclonal antibodies to keratins (K), including two recently dcveloped monoclonal antibodies raised to carboxy terminal peptides of K6 (LL020) and Kl6 (LL025). Keratinocytes from normal skin, untreated psoriatic plaques and non‐lesional psoriatic skin, were cultured using multiplein vitroSystems. Timelapse cinephotography was used to measure the intermitotic time of normal and psoriatic keratinocytes in both low calcium‐defined and serum‐containing media. The intermitotic time did not differ significantly between psoriatic and normal keratinocytes. Keratin expression of psoriatic and normal keratinocytesin vitrowas examined by both gel electrophoresis and immunocytochemistry. K6. K16 and Kl7 were detected suprabasally in all culture Systemsin vitro, but only in interfollicular psoriatic epidermisin vivo.and not in normal skin. Small subpopulations of keratinocytes expressed simple epithelial keratins K7, K8, Kl8 and K19 in cultures on plastic Substrates, but these keratins were absent in skin equivalents of normal or psoriatic skin. No psoriasis‐spesfic pattern of differentiation was foundin vitro. As the K6 peptide antibody reacted with basal cells of normal skin. probably due to K5 cross‐reactivity. K16 expression determined by LL025 was found to be the most sensitive indicator of the psoriatic state of differentiation. and this antibody is recommended for future work on psoriasis. Kl7 had a distinct pattern of tissue distribution in normal skin: Kl7. but not K16. was present in basal myoepithelial cells in sweat glands. and the dccp outer root shealh. but Kl 7 distribution parallelcd that ol'Klfi in suprabasal psoriatic epidermis. As keratins K6. Kid and Kl 7 are expressed in keratinocyte hyperproliferation. when high Ievels of certain cytokines are also expressed. the role of growth factors and regulalory nuclear transcription factors in the control of K6, K16 and Kl7 cxpression in psoriasis requires further study, in order to provide insight into the relationship between proliferation and differ

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb02696.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryIn a previous study. it was shown that the serum levels of type III procollagen aminoterminal peptide (P3NP) were significantly greater in patients receiving methotrexate (MTX) treatment for psoriasis than in untreated control patients with psoriasis. Although levels were highest in patients with hepatic fibrosis and cirrhosis, serum P3NP concentrations in those patients with nurmal liver histology on light microscopy were also shown to be significantly higher than in controls. In the present study, liver biopsies from 22 such ‘normal’ patients were examined hy electron microscopy, in order to determine whether P3NP levels correlated with ultrustructurally demonstrable fibrosis. Fibrosis in the perisinusoidal space of Disse was present in as many as 82% of biopsies. Although the prevalence of such fibrosis in psoriusis patients who have not received MTX is unknown, the high prevalence of Disse space fibrosis and of raised P3NP in MTX‐treated patients suggests that MTX causes subtle liver damage in a majority of treated patients. However. we were unable to show a statistical correlation between P3NP and the degree of Disse space fib

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb02697.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryWe have shown previously that IgG antibodies in certain pemphigus sera. particularly endemic Brazilian pemphigus foliaceus (BPF) sera. react with bovine desmocollins (Dsc). which are transmembranous glycoproteins of desmosome junctions. Desmocollins occur as three different isoforms (Dsc 1, 2 and 3). all of which are represented in the epidermis. In this study. we examined sera of various pemphigus types by immunoblotting purified bovine desmosomes and bovine Dsc l, 2 and 3 fusion proteins. expressed in pGEX expression vectors. Six of l5 (40.0%) BPF sera. two of 18 (11.1%) non‐endemic pemphigus foliaceus sera. eight of 39 (20.5%) pemphigus vulgaris (PV) sera. and two of l l (18.2%) normal sera. showed reactivity with Dsc from desmosomes. Experiments with fusioni proteins showed that no Dsc isoform was specifically recognized by sera of any individual pemphigus type. Our results indicate that the pathogenesis of pemphigus might be more complex than previously believe

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb02698.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryIt has been shown previously that N‐acetyl‐4‐S‐cysteaminylphenol (N‐Ac‐4‐S‐CAP) is a tyrosinase Substrate and a potent depigmenting agent of dark skin and black hair. The present study evaluated the depigmenting potency of an acetyl derivative of N‐Ac‐4‐S‐CAP. N‐2,4‐acetoxyphenyl thioethyl acetamide (NAP‐TKA) in the skin and hair. We tested for (i)in vitrometabolites in the skin after topical application. and (ii)in vivodepigmenting potency in the skin and hair. We found that NAPTEA was stable in water. but was converted to N‐Ac‐4‐S‐CAP after topical application to human skin. Therefore. although NAP‐TEA was not a tyrosinase substrate. it could react with tyrosinase after being converted to N‐Ac‐4‐S‐CAP by 0‐deacetylationin vivo.NAP‐TEA produced marked depigmentation of dark skin (Yucatan pig) after daily topical application. When given by intraper‐itoneal injection. it resulted in complete loss of hair colour (white) grown at the epilated site in adnlt C57 black mice after daily administration for l0 days, and incomplete loss of coat colour (silver grey) in newborn C57 black mice after a single administration, The depigmentation of the skin and hair was reversible, Splil‐dopa preparalion and eieclron microsnipy indicated thal lliis depigmentation is primarily related to (i) a marked decrease in the number of functioning melanocytes and melanized melanosomes, (ii) a decrease in the number of melanosomes transferred to keratinocytes, and (iii) selective degeneration/inactivation of melanocytes. and deposition of melanin‐like malerial in the Golgi cisternae. coated vesicles and melanosomes. where tyrosinase is reported to be located. We propose the NAP‐TEA is convertedin vivoto N‐Ac‐4‐.S‐CAP which. via interaction wit

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb02699.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryAdherence ofMalasseziayeast cells to human keratinocytes was assessed by a novel technique using double‐aided Sellotape. Although adherence using double‐sided Sellotape is still merely a model forin vivoadherence, it approximates to the conditions found on the skin surface. There were no differences in adhesive properties to human keratinocytes betweenMalasseziastrains originating from HIV‐positive and HIV‐negative patients with seborrhoeic dermatitis, nor was there a relationship between the severity of seborrhoeic dermatitis andin vitroadherence to human kerati

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb02700.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryEpstein‐Barr virus (EBV) is a gamma DNA herpes virus which is thought to play a part in the pathogenesis of some non‐Hodgkin's lymphomas in individuals with or without immunodeficiency. We investigated 16 lymph nodal and 12 cutaneous anaplastic large cell lymphomas (ALCLs) (Ki‐1+), all of which were in patients without immunodeficiency, for the presence of EBV genomes. The highly sensitive polymerase chain reaction (PCR) technique was employed for detection of viral DNA in extracts from formalin‐fixed, paraffin‐embedded tissue sections. In addition, we performed radioactive and non‐radioactive insituhybridization (ISH) for localization of EBV at the single cell level. EBV‐DNA was demonstrated by PCR in five cases of nodal ALCLs (31 %). All cutaneous ALCLs were negative. EBV‐encoded small nuclear RNAs (EBERs) could be identified by ISH in the tumour cells of one of the five EBV‐DNA‐positive patients. Our results further support the concept that EBV may be involved in the development of a proportion of nodal ALCLs, hut not

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb02701.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryIn order to identify more specific or selective mast cell markers, the reactivity of two monoclonal antibodies, Ki‐MC1 and Ki‐M1P, was studied by immunohistochemistry in two human cell lines (mast cell line HMC‐1, basophilic cell line KU812), in mast cells cultured from blood precursors, in adherent mononuclear cells from peripheral blood, and in mast cells of tissue sections from 1 3 urticaria pigmentosa lesions, live mastocytomas and live normal skin specimens. Toluidine blue staining, fluorescence staining with FITC‐conjugated avidin, and immunohistochemical staining (APAAP) with other mast cell reactive monoclonal antibodies, was performed for comparison. Double staining with the APAAP method, using the Ki‐antibodies and toluidine blue, was also carried out. Both Ki‐antibodies showed reactivity for skin mast cells, but with a different staining pattern. In addition, the Ki‐MC1 antibody did not react with the cell lines, and reacted only with a few peripheral blood mononuclear cells and cultured mast cells. In contrast, the Ki‐M1P antibody reacted with almost all cultured mast cells and blood mononuclear cells, but stained only about one‐half of lesional and one‐fifth of normal skin mast cells. Ki‐M1P also reacted with many toluidine blue‐negative dermal cells, particularly in urticaria pigmentosa. Ki‐MC1 antibody can thus be considered as a useful additional marker for normal skin mast cells. In contrast, the Ki‐M1 P antibody primarily identifies immature mast cells and monocytes/macrophages, suggesting that these cell types probably originate from the

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb02702.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryA 34‐year‐old Japanese man with hereditary sensory neuropathy was examined to evaluate the distribution, density and inter‐relationship between Merkel cells and peripheral nerves in the skin. An epidermal sheet of affected plantar skin showed numerous CAM 5.2‐reactive Merkel cells, whereas PGP 9.5‐reactive peripheral nerves were completely absent in the epidermis and dermis. These findings strongly suggest that Merkel cells do not require trophic maintenance from nerves in adult h

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb02703.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryThe value of DNA single cell cytometry for the detection of aneuploidy was assessed in 100 specimens of actinic keratoses and 39 specimens of Bowen's disease. Ten seborrhoeic keratoses and 10 samples of normal epidermis served as negative control groups. Monolayer smears, prepared from formalin‐fixed, paraffin‐embedded tissues, were Feulgen‐stained and used for interactive DNA‐cytometry. In each specimen, the DNA content of 150 randomly chosen squamous epithelial cells was measured, using a TV‐image analysis system (TAS‐plus, Leica, Germany). Aneuploidy was diagnosed if at least three nuclei with a DNA content above 5c (5cEE≥3) were found. The aneuploidy rate in actinic keratosis was 69% (69 of 100) and in Bowen's disease was 95% (37 of 39). Another 20 specimens of actinic keratoses and the remaining two specimens of Bowen's disease were diagnosed as suspicious for aneuploidy (0≤5cEE≤3). The 20 specimens of seborrhoeic keratoses nd normal epidermis did not show any nuclei above the 5c level, and were classified as non‐aneuploid. This indicates a sensitivity of 76% (106 of 139) and a specificity of 100% (20 of 20). The frequent occurrence of aneuploidy in actinic keratoses and Bowen's disease underlines the character of the lesions as epidermal carcinomas in situ, but does not explain the long‐term low inciden

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb02704.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummarySweat secretion, stratum corneum hydration and small nerve fibre function were measured in 40 patients with advanced chronic renal failure (CRF), using pilocarpine iontophoresis, electrical capacitance and a thermal sensory analyser which measures the thresholds of warm and heat pain sensation. Correlations were sought between these parameters, and the presence and severity of pruritus and skin xerosis were compared with 45 healthy control subjects. The mean sweat secretion and stratum corneum hydration of CRF patients were significantly lower than in controls. Thirteen patients had pathological thresholds to warm sensation on the foot, and eight on the hand. None had pathological thresholds to heat‐pain. The presence of pruritus did not correlate with any of the following: xerosis, stratum corneum hydration, sweat secretion or the results of thermal testin

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1995.tb02705.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY