摘要:

SUMMARYThe immunocytochemical identification and characterization of indigenous dermal dendritic cells (dermal dendrocytes) using a rabbit polyclonal antibody to clotting enzyme factor XIII subunit A (FXIIIa) was carried out on normal and inflamed human cutaneous tissue. The immunophenotype of FXIIIa positive dendritic cells was analysed with a panel of 18 monoclonal antibodies using immunoperoxidase and double immunofluorescence staining techniques.The antibody against FXIIIa detected highly dendritic dermal cells located particularly in the upper reticular and papillary dermis. Double fluorescence microscopy showed that FXIIIa positive cells were bone marrow derived (HLe‐I+) and co‐expressed monocyte, macrophage or antigen presenting cell markers (HLA‐DR+, LFA‐I+, HLA‐DQ+, OKM5+, MoI+, Mono‐I+, Leu M3+). No labelling was obtained with cell markers for Langerhans cells (CDI), T lymphocytes (CD2), granulocytes (LeuMI) fibroblasts (Te7), intercellular adhesion molecule‐I (ICAM‐I) or endothelial cells (Factor VIII related antigen).Gamma interferon induced increased expression of HLA‐DR and co‐expression of ICAM‐I on FXIIIa+dermal dendritic cells in normal skin in organ culture. Moreover, in benign inflammatory dermatoses such as atopic eczema and psoriasis there was an increased number of FXIIIa+, DR+, ICAM‐I+cells in the upper dermis and foci of FXIIIa+cells in the epidermis closely associated with lymphocytes.FXIIIa positive cells in human skin represent a specific population of bone‐marrow dermal dendritic cells, distinct from Langerhans cells, that share some features common to mononuclear phagocytes (monocyte/macrophages). In addition, the detection of HLA‐DQ on 48% of FXIIIa+cells and the lack of OKMI in combination with high OKM5 expression suggests an antige

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb15509.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYFoetal antigen 2 (FA‐2) is a connective‐tissue‐associated antigen isolated from second trimester human amniotic fluid. FA‐2 has an α‐electrophoretic mobility and is a single‐chain molecule with a molecular weight of 26 kDa as determined by polyacrylamide gel electrophoresis (PAGE). Using indirect immunofluorescence and the immunoperoxidase technique, FA‐2 was found to be in the lamina densa/ sublamina densa region of the basement membrane zone (BMZ) in adult as well as in foetal skin. FA‐2 was found throughout the dermis in foetal skin, whereas in adult skin it was found to be associated with the BMZ and around the blood vessels, hair follicles and eccrine glands. Intracellular FA‐2 antigen was demonstrated in proliferating fibroblasts by the indirect immunoperoxidase technique and immunoelectron microscopy of the fibroblasts revealed staining of the antigen in the cisternae of the rough endoplasmatic reticulum at the trans‐side of the Golgi complex as well as in vesicles close to the plasma membranes. FA‐2, a hitherto undescribed antigen associated with human BMZ, is probably being synthesized by pro

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb15510.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe transonychial water loss (TOWL) was measured in 21 healthy volunteers with an evaporimeter, to establish the usefulness of this technique and study the influence of sex, age and nail‐plate thickness. The median TOWL was 19.4 g/m2h−1and it decreased with increasing age (R = 0.5I, P<0.018). The median transepidermal water loss (TEWLHAND) from the back of the hand was 16.0 g/m2 h−1and from the dorsal side of the underarm (TEWLARM) it was 5.6 g/m2h−1. The TEWLARMwas significantly less than from any of the two other points studied (P<0.01), while there was no significant difference between the values obtained on the hand and the nail. TEWLARMhad a median value of 6.4 g/m2h−1in men and 4.3 g/m2h−1in women (P<0.05) in this study. No significant correlation between nail‐plate thickness, as measured by ultrasound 20 MHz A‐scan, and TOWL was found. Measurements of TOWL are of interest and should be age‐related. Further studies are needed to determine TOWL in various forms

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb15511.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe histopathological changes in the skin of 31 patients with the gravitational syndrome and 10 normal control subjects were studied. To quantify the clinical severity of the syndrome, a new index of severity was used. Histometric evaluation of the cutaneous vasculature was performed on biopsies taken from the edge of the ulcers in the 31 patients and from the medial aspect of the lower legs in control subjects. Endothelial cell mass did not correlate with the clinical index of disease severity, suggesting that angiogenesis did not necessarily signal healing. The mean luminal volume appeared to be of greater prognostic value as there was a significant negative correlation of this parameter with the index of clinical severity. The degree of epidermal hyperplasia was found to be related to both the amount of inflammatory cell infiltrate present and the endothelial cell mass per unit tissue volume. The results also showed that the clinical index of disease severity was positively correlated to the mean epidermal thickness. The quantitative evaluation of biopsy material can be used to provide prognostic information in the gravitational syndrome.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb15512.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYVirological studies were performed on 12 patients with epidermodysplasia verruciformis (EV). Three types of lesions were observed: red plaques, pityriasis versicolor (PV)‐like macules and plane warts. Human papillomavirus (HPV) 14, 20 and 21 were isolated from the plaques, HPV 3, 14 and 38 from flat warts and HPV 5, 12, 17, 20 and 38 from PV‐like lesions. No clear relationship could be established between the different lesions and the types of HPV. Types 17 and 20 have been isolated most frequently from Japanese EV patients and HPV 5, frequently detected in other countries, is less common, whereas HPV 8 has not been isolated. Skin cancers occurred in six of the cases (50%) and all had benign lesions that were PV‐like. At least one type of HPV 5; 17 or 20 could be isolated from these benign lesions and HPV 17 or 20 detected in the cancers. These three types of HPV in EV patients appear to be involved in the malignant transform

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb15513.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYA 61‐year‐old female patient with xeroderma pigmentosum (XP), registered as XP46KO, was assigned to complementation group F by the cell fusion‐complementation method. The XP46KO fibroblasts in culture exhibited a defective DNA repair capacity of 10–15% unscheduled DNA synthesis and a 3‐fold sensitivity to the lethal effect of 254 nm ultraviolet light compared with normal cells. The patient had mild clinical symptoms consisting of numerous pigmented freckles and a small number of scborrheic keratosis‐like papules. She had no skin cancers in the sun‐exposed areas of the skin and so far no neurological abnormalities. A review of 11 Japanese group F patients revealed very mild skin symptoms with no ocular or neuro‐psychiatric abnormalities. Single skin cancers occurred in only 3 of the 11 patients with an average age of 52 years for their first

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb15514.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe possibility of transmission of virus particles from patient to patient by multi‐use silver nitrate sticks and styptic pencils and by cotton wool swabs that have been dipped repeatedly into Dewar flasks of liquid nitrogen was studied. The results showed that transmission by the sticks and pencils in unlikely but that virus particles can be transferred into and out of Dewar flask on cotton wool swabs. We suggest that swabs should not be dipped repeatedly into the flask of liquid nitrogen but that, instead, a small aliquor of nitrogen should be decanted into a smaller ‘clean’ vessel and a new cotton swab used for each pa

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb15515.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYAn increased frequency of thyroid autoantibodies has been reported in patients with palmar and plantar pustulosis (PPP). This study was undertaken to determine the frequency and type of thyroid disease in 32 patients with this disease compared with a control group. Thyroid disease was demonstrated in 53% of the patients with PPP as compared to 16% in the matched control group. Fourteen patients with PPP had an enlarged thyroid and in six there were thyroid autoantibodies. There appears to be an increased incidence of goiter and thyroid autoantibodies in PPP with a decrease in the level of the thyroid hormones.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb15516.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYIn a double‐blind trial of topical 1,25‐dihydroxycholecalciferol in 47 patients with psoriasis no benefit was shown compared with plac

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb15517.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYA double‐blind, randomized multi‐centre study was performed to evaluate the efficacy of a 4% erythromycin and zinc combination (Zineryt®) versus 2% erythromycin (Eryderm®). One‐hundred and twenty‐two patients suffering from acne vulgaris were treated with either Zineryt® lotion or 2% erythromycin lotion. Acne grading and lesion counts for comedones, papules, pustules, nodules and macules were performed at each visit at 0, 1, 2, 4, 8 and 12 weeks. Treatment with Zineryt® lotion was found to be more effective than with 2% erythromycin as regards the reduction in number of the acne lesions and the severity grade

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb15518.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY