ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb14741.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryThe potential role of adhesion molecules in the pathophysiology of psoriasis was investigated and the pattern of expression of the cell‐surface receptors ICAM‐1, LFA‐3, and VLA‐1, 2, 3, 4, 5 and 6 was determined in biopsies of skin from patients with psoriasis (n=12) and from normal skin (n=12). There were no differences in the intensity or localization of the adhesion molecules VLA‐1, 2 and 4 and LFA‐3. In contrast, VLA‐3 and VLA‐6, which are restricted to the basal keratinocytes in normal skin, were overexpressed in the spinous cells in psoriatic skin. ICAM‐1 and VLA‐5, which are not expressed by keratinocytes in normal skin, were focally induced, especially in cells above elongated rete ridges and where there was an infiltrate with intraepidermal granulocy

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb14742.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryThe effects of minoxidilin vitrowere studied using fibroblasts grown from the lesional skin of patients with lichen sclerosus et atrophicus, morphoea and from the skin of normal individuals. The proliferation of all fibroblast lines over 3 days was inhibited in proportion to the concentration of minoxidil, being 20% or less of controls at 1 mM. where cell viability was only marginally reduced (84 ± 2% vs. 88 ± 2% (SEM) in controls). At 5 mM there was usually a net loss of cells and only 72% of those remaining were viable. In contrast, minoxidil at O·1–1 mM stimulated the proliferation of foreskin keratinocytes by up to 130%. Contraction of collagen lattices containing the three types of fibroblasts was inhibited by 22–26% with 1 mM minoxidil after 5 days and by 50–94% with 5 mM. Secretion of glycosanmnoglycans by normal fibroblasts showed concentration‐dependent reduction, being 25 ± 6% of that of untreated cultures with 1 mM minoxidil. These findings show that minoxidil has a range of inhibitory effects on both normal and abnormal skin fibroblastsin vitro, which contrast with its stimulation of skin epithelial cells, and support suggestions that it may provide a useful topical treatment for keloids and oth

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb14743.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryHairless mice were exposed to UVB irradiation from a Philips T1 12 light source. Mice that were exposed to UV‐light and not protected with 5%p‐aminobenzoic acid (PABA) showed a significantly higher number of peripheral blood granulocytes (P<0·001) and a significantly higher mean weight of both the spleen and the liver (P<0·001) than the non‐irradiated controls. light microscopy of the histology of the enlarged liver and spleen showed a proliferation of granulocytes and the reticuloendothelial cells. Treatment with topical PABA during the whole period of UV‐exposure prevented the peripheral blood granulocytosis. These protected mice also had a significantly (P<0·001) lower mean weight for the liver and spleen than UV‐exposed and non‐

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb14744.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryAn enzyme‐linked immunosorbent assay (ELISA) was developed to assess serum IgE antibodies directed againstPityrosporum ovalein patients with atopic dermatitis (AD), atopic patients with allergic respiratory disease (ARD: rhinitis or asthma) but without eczema, and in healthy controls. IgE binding toP.ovaleextract was demonstrated in 49% (35/72) of AD patients. In contrast, anti‐P. ovaleIgE was found in only one of 27 atopic controls without eczema: all healthy control sera (n=17) were negative. Of 37 AD patients tested intracutaneously withP. ovale.31 showed immediate‐type reactivity, and 20 of these 31 patients had anti‐P. ovaleIgE detectable by ELISA, while sera from the six non‐responders were all negative. Levels of anti‐P. ovaleIgE were highest in AD patients aged 20–30 years. No correlation was found with the severity of AD, but there was a non‐significant tendency (P=0.06) to higher levels in AD patients with concomittant respiratory allergy. Anti‐P.ovaleIgE was significantly correlated with total serum IgE, with specific IgE against various aeroallergens as measured by RAST, and with levels of anti‐Candida albicansIgE, measured with a similar ELISA. Thus, production of IgE antibodies againstP. ovaleoccurs very frequently in AD, and rarely in patients with atopic disease witho

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb14745.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryThe ultrastructural localization of the pemphigus vulgaris (PV) and pemphigus foliaceus (PF) antigens in cultured human squamous carcinoma cells was observed using immunogold electron microscopy. Both the PV and PF autoantibodies bound only to the extracellular portion of the desmosomal structures. After incubation at 37°C, the PV antigen–antibody complexes were observed within the cultured cells. PV and PF antigen expression was markedly reduced when the cells were cultured in medium with a low Ca2concentrati

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb14746.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryAtypical intraepidermal T cells in Pagetoid reticulosis did not express CD45RO, the low molecular isoform of CD45. These cells did express other CD45 isoforms in the disseminated aggressive variant (Ketron‐Goodman type). The complete CD45 family was lost from the cell surface in the henign localized variant (Woringer‐Kolopp type). Because the presence of CD4S is crucial for the function of the lymphocyte‐specific tyrosine kinase p56lck, which is associated with lymphocyte growth and transformation, this may be the molecular basis for the non‐aggressive nature of this clonal T‐cell prol

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb14747.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryThe transcutaneous partial pressures of oxygen (tcpO2) and carbon dioxide (tcpCO2) were measured at eight different sites in 10 adult male subjects with an electrode at a temperature of 44°C. The mean tcpO2values (mmHg) were significantly lower on the face (forehead 26·6, cheek 29·6) and the palm (26·4) than at other sites (60·6–69·6), The tcpCO2values (mmHg) were only slightly higher on the face. Removal of the stratum corneum produced an average increase of the tcpO2on the palm of 37·6 mmHg and on the forehead of 19·6 mmHg. However, in 10 children with an age range of 3–9 years, the difference in the mean pO2between the cheek and forearm was very small. There was no significant difference in the cutaneous blood flow at 44°C between the cheek, palm

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb14748.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryThe psychological characteristics and the hair problems of 58 females with androgenetic alopecia were compared with a group of women with non‐apparent dermatological diseases, and with a group of men with androgenetic alopecia. The women with androgenetic alopecia had higher scores for self‐sufficiency and social inadequacy compared to women with non‐visible dermatological complaints, and they scored higher for inadequacy, rigidity and general psychological maladjustment than the men and had lower scores for injuredness self‐evaluation and self‐esteem. The women with androgenetic alopecia had more psychosocial problems, which they attributed to the hair loss, than the oth

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb14749.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryFifty patients with psoriasis undergoing a standard Ingram regime were entered into a double‐blind comparison of oral indomethacin and placebo. No significant differences were observed between the two groups in respect of disease activity or dithranol tolerance after 6 week

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb14750.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY