ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb05706.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYLH 7:2 is a monoclonal antibody that was raised against an extract of human epidermal cells and identifies an epitope within the lamina densa of the basement membrane of stratified squamous epithelia. Using indirect immunofluorescence we found intense labelling with LH 7:2 at the epidermal basement membrane (EBM) of normal skin, and in skin samples from patients with simplex, junctional, dominantly inherited dystrophic and acquired forms of epidermolysis bullosa (EB), as well as bullous pemphigoid. Staining was absent or only very faint in generalized recessive dystrophic EB (RDEB), and patchily reduced in the localized form of RDEB.We conclude that LH 7:2 recognizes an EBM antigen which may be important in the pathogenesis of RDEB. Moreover, the antibody provides a useful probe for the rapid diagnosis of RDEB and is of special value in helping to discriminate between localized RDEB and typical dominant dystrophic EB—conditions which closely resemble each other clinically and which cannot be distinguished by means of transmission electron microscop

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb05707.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYA conditioned medium from cultured human epidermal cells was observed to inhibit the activity of exogenous urokinase. By reverse fibrin autography after SDS polyacrylamide gel electrophoresis, a plasminogen activator inhibitor was detected with a molecular weight of 46000. Using Mr‐ 33000 [125I]‐labelled urokinase we observed the formation of an enzyme‐ligand complex. The molecular weight of this complex was 79000. These results indicate that cultured human epidermal cells secrete a plasminogen activator inhibitor (urokinase inhibitor) with a molecular weight of

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb05708.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYFibroblasts derived from patients and animals presenting various heritable connective tissue disorders were investigated for the ability to retract a reconstituted collagen matrix. When seeded into gels, dermatosparactic calf and sheep fibroblasts did not exhibit the elongated shape of normal fibroblasts and did not contract the collagen lattice to the same extent as control fibroblasts. In contrast, several cell strains obtained from patients with Ehlers‐Danlos syndrome type VII displayed contractile properties for collagen gels similar to controls. Delayed contraction was noted by two strains of fibroblasts from patients with Ehlers‐Danlos syndrome type IV, whereas fibroblasts from patients with osteogenesis imperfecta, Marfan syndrome and cutis laxa had normal retraction propert

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb05709.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYLevels of thymosin alpha I in the sera of 37 patients with Brazilian pemphigus foliaceus (BPF) were measured using a competitive binding radioimmunoassay. The values were compared with 19 patients with other forms of pemphigus, 13 relatives of patients with BPF, 18 patients with other dermatological diseases, and 265 normal controls. We found that 27 (73%) of the patients with BPF had thymosin alpha I serum levels that were at least two standard deviations above the mean for normal individuals. The mean value for patients with BPF was significantly greater than any other groups studied. The thymosin elevation is similar to alterations seen in certain viral diseases and suggests that BPF is aetiopathogenically distinct from the other forms of pemphigus.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb05710.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYBiopsies from 63 patients, 40 with mycosis fungoides (MF) and 23 with other lymphocytic infiltrates, were stained with a panel of monoclonal antibodies to differentiation antigens (OKT10, Leu 8), to activation antigens (OKT9), and to an antigen expressed by all dividing cells (Ki67) to establish whether this panel was of value in the diagnosis of mycosis fungoides. None of these antibodies used in isolation was found to give a staining pattern specific for mycosis fungoides, but the phenotype Leu 3a +, OKT9+, Leu 8 ‐ of the majority of lymphocytes in the cutaneous infiltrate was strongly suggestive. The presence of Leu 3a + Leu 8 ‐ lymphocytes is of particular interest in that in the peripheral blood both of normal subjects and of MF patients Leu 3a + lymphocytes also are predominantly Leu 8 +, as were the lymphocytes in the cutaneous infiltrates of the non‐MF infiltrates included in this

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb05711.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYIn six patients with cutaneous T‐cell lymphomas (CTCL), the effect of leukapheresis (LPH) on the disease and on various immunological and haematological parameters was investigated. Enriched mononuclear cells were removed by continuous centrifugation leukapheresis from the peripheral blood of the patients. A profound and longlasting effect was seen in one patient with the Sézary variant; a moderate response was seen in two other Sézary patients. No benefit was observed in two patients with more aggressive leukaemic disease, and one patient with plaque stage MF responded on two occasions. No limiting side effects were recognized and no consistent changes of immunological or haematological parameters were observed after

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb05712.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYA survey of 500 patients hospitalized in non‐dermatology wards and referred for dermatological opinion is reported. In approximately 50%, the skin lesions were related to the presenting illness; in 36.6% of these patients the skin condition contributed substantially to the diagnosis of the systemic disease. This study emphasizes the considerable interface between cutaneous and systemic disease and the need for close co‐operation between medical and dermatology departments. The implications for postgraduate education are highlighted, since such patients are infrequently seen in a dermatology out‐patient se

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb05713.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYData on 42 patients with brachioradial pruritus seen in Hawaii over a 2‐year period are presented. In contrast to previous reports, the sex ratio was 1:1 with a preponderance of patients in the younger age groups. All patients had developed the disorder after having lived in Hawaii for at least one year. More than half the cases had no history of neck trauma or arthritis, ruling out a peripheral neuropathy as the cause. Radiographs of 15 patients showed changes only in the patients in the older, arthritis‐age groups. Brachioradial pruritus appears to be peculiar to the trop

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb05714.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYThe enzyme elastase (EC 3.4.21.37) has proved to be a convenient and extremely sensitive marker for the quantification of neutrophils in cutaneous infiltrates. Fluorometric assay using the synthetic substrate MeOSuc‐Ala‐Ala‐Pro‐Val‐N‐methylcoumarin permitted the measurement of this enzyme in as few as five cells and was linear up to about 1000 cells per sample. The mean activity of lysates of human blood‐derived neutrophils was 0.57 ± 0.08 pmol of 7‐amino‐4‐methyl‐coumarin released per hour per neutrophil. Extracts of normal human skin contained no measurable elastase activity but resulted in a slight inhibition of the neutrophil enzyme (mean 12%).Application to thein vivosituation has been demonstrated by the use of leukotriene B4as chemotactic agent. A reproducible neutrophil infiltrate was found at a dose of 2 ng, well below the threshold for the appear

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb05715.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY