ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb00590.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryThe role of substance P (SP) in allergic reactions of the skin was investigated. Spantide, a competitive inhibitor of SP. was injected intracutaneously into the volar aspect of the forearm prior to the following challenges: benzalkonium chloride (irritant delayed reaction), tuberculin (immunological delayed reaction). UVB irradiation, benzoic acid (non‐immunological contact urticaria), different food allergens and latex (in patients with immunological contact urticaria). Only the immunological reactions of contact urticaria and the reaction to tuberculin were suppressed by the SP antagonist, indicating that SP is involved in their pathogenesi

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb00591.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummarySuction has been used to investigate cell adhesion in clinically normal skin in Hailey‐Hailey disease. We have demonstrated that there is a widespread subclinical abnormality in keratinocyte adhesion in this disease. There may be a synthesis of functionally deficient adhesion junctions, increased breakdown of adhesion junctions or abnormalities in other adhesion proteins in the epidermis In Hailey‐Hailey disease. The findings contrast with those in Darier's disease In which abnormal cell adhesion was only demonstrable in clinically involved

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb00592.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryThein vitroeffects of liposomes on HaCaT human keratinocytes were studied with regard to their uptake, lipid fluidity and proliferation of the cells. Oligolamellar liposomes, prepared from soya bean phospholipids. had a mean size of 150nm and consisted predominantly of phosphatidylcholine (83%) and phosphatidylethanolamine (10%) and the fatty acids comprised mainly linoleic acid (66%) or other unsaturated fatty acids. After 6 and 24 h of incubation with 1 and 0–1% w/v of liposomal lipids, phase‐contrast microscopy revealed marked cytoplasmic vacuolization of the cells. Keratinocytes treated with the liposomes contained aggregations of multilaminated lipid material without delimiting cell membranes. The cellular lipid fluidity (reciprocal of diphenylhexatriene fluorescence polarization P‐value) correlated with liposomal concentration and incubation time. A significant elevation of lipid fluidity (P1% for protein content per well after 24 h of exposure. The cells were able to proliferate and lipid fluidity returned to normal within 7 days following discontinuation of incubation with l

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb00593.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryGlucocorticoids increase the β‐adrenergic adenylate cyclase response of epidermal keratinocytes. Using FRSK cells, a cultured cell line of foetal rat keratinocytes, the regulatory mechanism of the β‐adrenergic augmentation effect was investigated. Treatment with dexamethasone (1 × 10−6M)increased by 1.5‐fold the β‐adrenergic adenylate cyclase response of FRSK cells. The effect was observed at 6 h incubation and remained for at least 48 h. The prostaglandin E‐adenylate cyclase response was also increased 1.5‐fold by glucocorticoid treatment. Neither the adenosine‐adenylate cyclase response nor cholera toxin‐ or forskolin‐induced cyclic AMP accumulations were altered. Northern blot hybridization showed that levels of the β2‐adrenergic receptor mRNA increased within 3 h. while actin‐, Gs‐α, Gi‐2α, Gi‐3α mRNA levels were unchanged. Testosterone. 17β‐oestradiol. and progesterone had no effect on either the β2‐adrenergic adenylate cyclase response or the expression of β2‐adrenergic receptor mRNA. The increase in the numbers of the β‐adrenergic receptors was visualized by immunofluorescence with an antibody specific for the β2‐adrenergic receptor. Our results indicate that glucocorticoids regulate the β2‐adrenergic adenylate cyclase response of FRS

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb00594.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryThe histological features of biopsies from 18 previously unreported cases of Sweet's syndrome are reported. The dermal infiltrate in the majority of the cases contained numerous histiocytes that at first sight appeared to mimic neutrophils. The immunophenotype of these histiocytes was consistent with monosytes that have freshly infiltrated into the lesions. Only two of the cases in this series, associated with leukaemia, displayed the histological features of Sweet's syndrome with a predominant neutrophilic infiltration.We suggest that the initiating mechanisms in Sweet's syndrome are that monocyte/histiocyte derived cytokines such as the interleukins IL‐1 and IL‐8. secreted either by infiltrating histiocytes in the non‐leukaemia‐associated cases of Sweet's syndrome or by tumoural myelomonocytic cells in those associated with leukaemia, are responsible for the systemic manifestations and the infiltration with neutrophils in the skin

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb00595.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryThe use of topical nitroglycerin has been reported to aid the healing of chronic skin ulcers. In this study, the effect of 2% nitroglycerin ointment on transcutaneous oxygen pressure (TcPO2) was investigated in skin adjacent to the area of ointment application. Topical nitroglycerin caused a two fivefold decrease in TCPO2in skin next to or up to 9 cm away from the site of application of the ointment.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb00596.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryPercutaneous absorption of hydrocortisone was measured in four children and three adults with atopic dermatitis after the application of 1% hydrocortisone cream and again 12 h alter the application of a moisturizer containing 80% water and 5% propylene glycol to the same areas. A significant increase in the level of the plasma cortisol was observed after both applications and these levels were still elevated at 24 h. Topically applied hydrocortisone. Stored in eczematous skin, could be released from this reservoir by a moisturizer containing propylene glycol.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb00597.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryThe effect of isotretinoin (Roaccutane) on serum steroids and urinary steroid metabolites was investigated in seven female patients receiving the drug for treatment of severe acne over a 16‐week period. Serum concentrations of dehydroepiandrosterone sulphate (DHAS), androstenedione (A2), and free androgen index (FAI) were not significantly altered. There was a significant fall in testosterone during treatment and a significant reduction in the 24 h urinary excretion of androsterone, tetrahydrocortisone (THE) and tetrahydrocortisol (THF) from week 8 onwards and for aetiocholanolone and allo‐THF from week 12 (P<0.05). Although pretreatment levels of urinary steroid metabolites were not abnormal, the ratios of the 5α/5β metabolites (androsterone:aetiocholanolone and allo‐THF:THF) were at the upper limit of the reference range and were lowered after treatment, suggesting that 5α‐reductase activity is sensitive to is

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb00598.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

SummaryInformation concerning the operation of 134 PUVA units in 96 dermatology centres in the U. K. was collected by a postal questionnaire. The most common protocol was to give PUVA three times weekly in the treatment of psoriasis, and to choose the initial dose of UVA according to skin type. However, the initial UVA dose encompassed a range of five or more within each skin type.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1991.tb00599.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY