摘要:

SUMMARYCorticosteroids suppress delayed‐type hypersensitivity (DTH) reactionsin vivoand impair lymphoid cell functionsin vitro.In contact hypersensitivity (CHS) to corticosteroids, however, the corticosteroids are capable of inducing DTH responsesin vivo.The present study examined the capacity of corticosteroids to induce in vitro proliferation of T lymphocytes from patients with CHS to corticosteroids. With peripheral blood mononuclear adherent cells as antigen‐presenting cells (APC) and hydrocortisone‐17‐butyrate (H‐17‐B) as hapten, no proliferation responses were detected of T lymphocytes from patients with CHS to H‐17‐B. However, when epidermal Langerhans cells (LC) were used as APC, weak proliferation response

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb04186.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYSkin samples taken from foetuses of 15–19 weeks gestational age and keratinocytes were cultured by the 3T3 feeder method or in serum‐free MCDB 153 medium on 16 mm coverslips. Keratinocytes taken from paediatric circumcisions and patients undergoing plastic surgery were also cultured using the 3T3 feeder method. A panel of monoclonal antibodies against a number of cytokeratins and differentiation markers were used in the PAP technique to analyse the cells. Cryostat sections taken from the donor skin samples were stained simultaneously. Foetal skin expressed the cytokeratins 7, 13 and 19 that were not observed postnatally. This cytokeratin expression was maintained in both culture conditions and also observed in paediatric and adult keratinocytes. Cytokeratin 7 was expressed on a greater proportion of foetal cells thanin vivo, whereas the expression of 13 and 19 decreased. All keratinocytes expressed vimentin, transferrin receptor and Ki67 (proliferating cell antigen), while a small proportion expressed involucrin throughout culture, indicating their high level of differentiation and proliferation. No differences were observed between low and high density cultures. Foetal keratinocytes cultured in MCDB 153 did not reach confiuence and stronger staining of differentiation markers was observed in these cells. These results show that thein vivodifferences in cytokeratin expression of foetal and adult keratinocytes disappear in cult

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb04187.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYHuman hair follicle mesenchyme contains a distinctive extracellular matrix which varies in volume and composition in concert with the hair growth cycle. To investigate the possibility that mesenchymal function is disturbed in alopecia areata we have studied the expression of extracellular matrix constituents in scalp biopsies from 14 patients by immunohistochemical staining for basement membrane proteins, proteoglycans and interstitial collagens. The staining patterns in follicles from non‐lesional scalp were normal. Miniature anagen follicles from bald patches also showed relatively normal expression of basement membrane proteins and proteoglycans. However, in some large anagen follicles from lesional sites, there was loss of the normal staining pattern for chondroitin‐6‐sulphate in the dermal papilla. In lesional Catagen follicles, the glassy membranes showed marked convolution and thickening. These structures stained strongly for laminin and type IV collagen but only weakly for interstitial coll

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb04188.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYTo determine whether the clinical, immunological and serological features of patients with silica‐associated systemic sclerosis are different from patients with the‘idiopathic’ form of systemic sclerosis (SS) we studied 22 underground coal miners who were exposed to silica dust (SD), 30 mine workers who later developed silicosis (S) and 17 mine workers exposed to silica dust who subsequently developed a systemic sclerosis‐like disease (SA‐SS).The patients with SA‐SS had features clinically indistinguishable from individual patients with SS. They all had Raynaud's phenomenon, 14 had cutaneous sclerosis identical to that seen in acrosclerosis and three had a generalized cutaneous sclerosis. Sixteen patients had bibasilar pulmonary fibrosis, 10 had necrosis of the fingertip pulps, nine had oesophageal involvement and only one patient had renal involvement. Antinuclear antibodies and circulating immune complexes were detected in three and eight patients with SD, 14 and five patients with S and in 16 and nine patients with SA–SS, respectively. Anti‐Scl‐70 antibody was detected in eight of the 17 patients with SA‐SS. Evidence forin vivoendothelial cell damage, as determined by elevated levels of von Willebrand factor, was found in nine patients with SD, 14 patients with S and in 10 patients with SA‐SS. Following incubation of the patient's serum with confluent cultures of human umbilical vein endothelial cells there was only a significant reduction in calcium ionophore‐induced release of prostacyclin with the serum from SA‐SS patients compared to that with control serum (NC). The mean ± SEM release of 6‐keto‐PGF1α (the stable metabolite of prostacyclin expressed as ng/104cells) decreased from 2·900·27 to 2·01±0·33 (SD), 3·34±o·42 to I·76±0·31 (S), I·98±12 to 0·64±0·07(SA‐SS) and 2·28±0·33 to 1·36±0·21 (NC) with 1 and 20% serum, respectively.This study demonstrates that immune complex and antinuclear antibody formation andin vivoendothelial cell damage occurs following occupational exposure to silica. The patients who subsequently develop a systemic sclerosis‐like disease have clinical, immunological and serological features which are indistinguishable from the idiopathic form of the disease although as a group the SA‐SS patients have a higher preval

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb04189.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYThe mechanism of the occurrence of natural antikeratin antibodies in human sera was studied using hybrid spleen cells obtained from experimentally naive or from immunized mice. Antikeratin antibodies were detected by enzyme‐linked immunosorbent assay (ELISA) in 5·9–9·5% of the culture supcrnatants of fused spleen cells taken from naive mice. When mice were immunized with keratins, the number of supernatants containing antikeratin antibodies was increased to eight out of 51 (15.7%). When immunized with non‐keratin materials such as activated human T cells, adult T‐cell leukaemia cell lysates, and human T‐cell lymphotropic virus type‐1 (HTLV‐1), 16·7–20·8% of the supernatants were found to contain antikeratin antibodies by ELISA.The antikeratin antibodies in the supernatants showed cytoplasmic staining of keratinocytes in human as well as mouse skin by indirect immunofluorescence. The antibodies reacted with extracted human epidermal keratins by dot‐blot and Western blot analysis. Most antikeratin antibodies in the supernatants did not show cross‐reactivity with exogenous antigens used for immunization and vimentin‐type intermediate‐sized filaments.The findings demonsrate that B cells producing antikeratin antibodies are common in naive mice, and produce various type of antikeratin antibodies following specific activation with epidermal keratins and non‐s

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb04190.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYA Study of out‐patient dermatological services (NHS and private) in the south‐east of Scotland was carried out by medical staff in the Department of Dermatology in Edinburgh during the month of November 1988. The aim was to assess changes in referral patterns and workload compared with the findings of an identical investigation undertaken in November 1981. Of particular interest were the possible effects of recent publicity campaigns aimed at increasing public awareness about skin cancer.The medical complement of the dermatology department had changed minimally since 1981 and the population increase in the south‐east of Scotland over the same period was 1·5%. During November 1988 1592 new patients and 2037 review patients were seen. This represented an increase of 29·2% and 28·3%, respectively, since 1981. The most striking changes in diagnostic groups were a 173% rise in new cases presenting with benign tumours (excluding viral warts) and a 106% increase in new patients with malignant tumours. Viral warts and eczema were, as in 1981, the second and third most common diagnostic categories amongst new patients. There was a 98% increase in the number of surgical procedures performed on new patients compared with 1981. We conclude that the substantial increase in numbers of both benign and malignant tumours and the consequent doubling in surgical treatments was due to increased public awareness and concern about ski

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb04191.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYA prospective cross‐sectional questionnaire study of 32 patients with psoriasis was carried out in order to validate the use of the Sickness Impact Profile (SIP) in psoriasis and compare its sensitivity with the Psoriasis Disability Index (PDI). Overall PDI scores, but not overall SIP scores, correlated well with PASI scores (P<0·05). There was good correlation between the PDI and overall SIP scores (P<0·01). Psychosocial factors are more severely impaired than physical activities in patients with psoriasis.It is now possible to directly compare the disability experienced by psoriatic patients with that experienced by patients suffering from other systemic diseases, using the SIP. The PDI is an appropriate method to give a rapid overall measure of psoriasis disabil

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb04192.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYWe report 22 cases of pigmented spindle cell naevus (PSCN). The usual appearance of these naevi is that of a heavily pigmented papule found mostly on the legs of young patients. Histologically, PSCN was characterized by symmetrical proliferation of spindle‐shaped pigmented melanocytes grouped in large junctional nests. Pagetoid spread of single cells in the overlying epidermis was frequently found. In our opinion, PSCN is a distinctive benign acquired melanocytic naevus that in the past has been frequently misdiagnosed as atypical Spitz naevi or malignant melanom

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb04193.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYUsing an antibody to S100 protein, the number of dendritic cells above the basal layer in the epidermis was assessed in necrobiosis lipoidica and granuloma annulare. A statistically significantly higher number of these cells was found within the epidermis in necrobiosis lipoidica compared with granuloma annulare and normal skin. The numbers were similar to those seen in sarcoidosis and tuberculous reactions in the skin, which raises the possibility of an immune pathogenesis for necrobiosis lipoidica.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb04194.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYThirty‐four dermatology out‐patients with chronic idiopathic urticaria and 34 with idiopathic generalized pruritus were investigated using standardized self‐assessment psychological questionnaires to determine the incidence of significant symptoms of depression and anxiety. These patients were compared with age‐ and sex‐matched but otherwise unselected general dermatology out‐patients. Using the Beck depression inventory, significantly more patients with generalized pruritus (32·4%) had depressive symptomatology (score>14) than controls (13·2%),P<0·05). Although more patients with chronic urticaria bad depressive symptomatology (14·7%) than controls (4.4%), the difference was not statistically significant. Using the Speilberger state‐trait anxiety inventory there were no significant differences between the patients with pruritus or urticaria and their controls with respect to state or trait anxiety scores above the upper 90%, probability limit for the general population. Thus, significant depression may be expected in a substantial proportion of patients witb idiopathic generalized pruritus but in a relatively small proportion of those with

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb04195.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY