摘要:

SUMMARYIn a Study of twenty‐five patients with herpes gestationis we found that 80% possessed the HLA antigen DR3, which confers increased immune responsiveness and a predisposition to ‘auto‐immune disease’. In five patients the development of herpes gestationis coincided with a change in sexual partner, suggesting that the development of herpes gestationis may depend on exposure to an antigen derived from the father. This might share determinants with a component of the basement membrane zone of skin. Although anti‐basement membrane zone antibodies are present in HG it is not clear whether they play a pathogenic role. The infrequency of neonatal involvement and the lack of correlation between immunofluorescence findings and clinical activity in our patients suggested that the antibodies might be a result of tissue damage rather than its cause. Two patients In our study were exceptional in that episodes of herpes gestationis were followed by normal pregnancies. In these patients the relationship of their DR antigens to those of the fetus may have been important in determining whether or not the pregnancy would be affected by herpes ge

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07073.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYAn indirect immunofluorescence assay using formalin‐fixed paraffin‐embedded skin was performed on six biopsies from four patients with eosinophilic cellulitis (Wells' syndrome) to determine the extracellular localization of eosinophil granule major basic protein (MBP). Serial sections from each biopsy were treated with either affinity chromatography‐purified antihuman‐ MBP or staphylococcal protein A purified rabbit IgG (control material). There was striking extracellular fluorescence localized to flame figures, and intracellular staining of eosinophils in all sections treated with anti‐MBP as compared with controls. The pattern of MBP extracellular staining corresponded to the configuration of each flame figure (as verified by counterstain of the same section with haematoxylin and eosin). These findings show that MBP can be used as a marker for determining eosinophil degranulation and, because MBP is localized to flame figures, they suggest that MBP may play a pathogenic role in Wells'

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07074.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYFibroblasts were cultured from the involved and uninvolved forearm skin of patients with severe generalized psoriasis and compared with those from the forearms of normal controls of similar ages. Thirteen strains were obtained from involved skin (PSA strains) and sixteen strains from uninvolved skin (PSB), with thirteen control strains (NSF).Outgrowth of fibroblasts from the psoriatic skin explants was slightly quicker than from control skin and the average proliferation rates of passaged strains were PSA 144, PSB 134 and NSF 94 (P<0.05). Psoriatic fibroblasts were abnormally dependent on serum for anchorage. In serum‐free medium many cells rounded up and were only loosely attached to the substratum. This effect was rapid, reversible and not corrected by adding fibronectin. Cell attachment assays showed only small differences between the psoriatic and normal fibroblasts and the main effect of serum withdrawal appeared to be on spreading rather than attachment.These data suggest that the dermis of both involved and uninvolved psoriatic skin is abnormal and that the hyperactivity persistsin vitro.Our findings seem most compatible with a hyperproliferative reaction of both epidermis and dermis to an extracutaneous, perhaps vascular, stimulu

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07075.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYGlycosaminoglycans and protein synthesis were assessed in fibroblasts cultured from the involved (PSA strains) and uninvolved (PSB strains) forearm skin of patients with severe generalized psoriasis and compared with that in fibroblasts from the forearm skin of control subjects of similar ages (NSF strains).Average rates of glycosaminoglycan secretion were 19% (PSA) and 28% higher (PSB) in psoriatic strains. Although these differences were not significant in themselves, the relationship between glycosaminoglycan secretion and fibroblast density (cells/cm2) was significantly different (P=0.02) for the psoriatic and control groups, with the psoriatic cells secreting appreciably more glycosaminoglycans at higher cell densities. The nature of the glycosaminoglycans produced was the same in psoriatic and normal strains; 90% was recovered in the culture medium and the bulk of this appeared to be hyaluronic acid with only small amounts of sulphated components. Collagen synthesis was 77% higher in PSA and PSB strains (P<0.01), while other protein synthesis was increased by 44% (P<0.05).These findings confirm that fibroblasts from psoriatic skin are hyperactivein vitro.The data are compatible with the results of studies of psoriatic skinin vivoand suggest that the entire skin is abnormal.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07076.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYIn a case‐control study, 287 women with malignant melanoma were compared with 574 age‐matched controls. Red hair colour at age 5 years was associated with a tripling of risk [relative risk (RR) = 3.0], blonde hair with a 60% increase (RR=1.6) and fair skin with a doubling (RR = 2.1). Women with melanoma also reported that they tended to burn (RR = 1.4) and to freckle (RR =1.9) after exposure to sunlight. Since fair skin, red hair, and the tendency to burn or freckle after exposure to sunlight all cluster in the same individuals, the extent to which each of these factors had an independent influence on susceptibility to melanoma was investigated. Hair colour, especially red hair, proved to be the major determinant, followed by skin colour.The reporting of above average numbers of naevi on the body was as strong a predictor of melanoma as was red hair colour (RR = 3.4). A history of psoriasis was also more common in cases than controls (RR = 3.0) as was a history of vitiligo (RR= 1.8). A history of acne appeared to be protective (RR = 0.4) as did a history suggestive of chloasma (RR = 0.6) and premature greying of the hair (RR = 0.6). These relationships were irrespective of hair and skin col

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07077.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYDNA frequency distributions analysed by single nuclei flow cytometry were studied in sixty‐five curetted human epidermal tumours, i.e. five actinic keratoses (AK), seven Bowen's diseases (BO), nine squamous cell carcinomas (SCC), forty‐three basal cell carcinomas (BCC) and one baso‐squamous carcinoma (BSC). Seventy‐five per cent (16/21) of the samples with squamous cell differentiation (AK, BO and SCC) showed features suggestive of more than one stem cell population, against 24% of the pure BCC samples (11/43).The DNA indices for the tumours, i.e. the ratio between the DNA content of the tumour stem cell line G1cells and normal epidermis G, cells were calculated. For BCC and SCC a preponderance was found for near‐diploid and near‐tetraploid cell clones. The precancerous lesions contained clones with more broadly scattered DNA indices.The fractions of cells in S and G2M and S + G2M phases were calculated for the samples with only one detectable stem cell population. For the squamous cell tumours and the nodular (but not the superficial) BCC, these fractions were significantly different from the unaffected skin of patients with multiple epidermal cancers. The usefulness of cell cycle fraction determinations for curetted tumours i

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07078.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYSeventeen biopsies from contact allergic reaction sites in sensitized patients were studied by electron microscopy and immunocytochemistry. Very intimate appositions of macrophages to T lymphocytes were frequently observed in the dermis at 6, 8 and 12 hours after application of the allergen. Such juxtapositions were seen less frequently after 24, 48 and 72 hours. These appositions seem to indicate that macrophages play an antigen‐presenting role in human contact allergic reactions, as Langerhans cells seem to d

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07079.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYThe comedogenicity of UVA‐irradiated and non‐irradiated substances (squalene, oleic acid, tetradecane, isopropyl myristate, squalane and liquid paraffin) was evaluated by surface microscopy and histological examination after treating the ventral skin of rabbit ears with these substances. The lipid peroxide levels of these substances were also measured. Squalene itself was scarcely comedogenic but squalene peroxides were highly comedogenic. Both oleic acid and its peroxides were able to induce fairly large comedones and there was a good correlation between the lipid peroxide levels and the size of the comedones. The specimens biopsied from these comedones showed marked hyperplasia and hyperkeratosis of the epithelium in the follicular infundibulum and marked proliferation of the sebaceous glands.Although free fatty acids might play a role in the pathogenesis of acne, it is proposed that squalene and free fatty acids in sebum may be less comedogenic than their peroxi

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07080.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYT‐ and B‐lymphocyte subpopulations were measured in the peripheral blood of patients with varying severity of acne and in acne‐free control subjects. Increased severity of acne correlated with an increased number of T lymphocytes, helper T cells and B cells, while the number of suppressor T cells remained normal and did not alter significantly. This evidence suggests that there is no lack of regulation of the immune response in acne pat

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07081.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYOral psoralen photochemotherapy (PUVA) was found to be effective in five patients with chronic hyperkeratotic dermatitis of the palms. Relapses may occur but they respond well to retreatment.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07082.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY