ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb02842.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYThe percentage of non‐diploid epidermal cells was determined by flow cytometry following application of leukotriene B4(LTB4) to human skin. Doses in the range 35–500 ng were shown to cause a marked increase in proliferation, the non‐diploid cells reaching a maximum between 72 and 96 h after LTB4application. No difference was observed between the response of healthy controls and the uninvolved skin of psoriatic patients. We suggest, therefore, that the hyperproliferation is a consequence of the physical disruption of the stratum corneum accompanying the rupture of microabsc

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb02843.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYThe effects of topical PUVA treatment on the epidermal cyclic AMP system were investigated. 8‐methoxypsoralen (8‐MOP), 0.3% in ethanol was applied to the backs of pigs which were then irradiated with UVA. A significant increase in the epidermal beta‐adrenergic adenylate cyclase response was observed 24 h after low (1.1 J/cm2) and moderate (2.1 J/cm2) dose irradiation. There was no significant change in the adenosine‐ or histamine‐mediated adenylate cyclase responses. 8‐MOP application or UVA irradiation alone had no effect on the beta‐adrenergic adenylate cyclase response. PUVA treatment with a higher irradiation dose (4.2 J/cm2) produced no increase in the beta‐adrenergic response and adenosine‐ and histamine‐mediated adenylate cyclase responses were decreased. Cyclic AMP phosphodiesterase activity was decreased by PUVA treatments using UVA doses of 1.1 and 2.1 J/cm2; however, the change was not statistically significant. The increased beta‐adrenergic response was also observed in the presence of the cyclic AMP phosphodiesterase inhibitor, isobutylmethylxanthine. These results indicate that epidermal adenylate cyclase responsiveness is affected by topical

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb02844.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYDistinctive patterns of skin disease other than Kaposi's sarcoma have been seen in patients with AIDS, in others with persistent generalized lymphadenopathy (PGL) and in a group at high risk of developing AIDS. We found a chronic acneiform folliculitis on the face, back, chest and buttocks, extensive cutaneous fungal infections and a striking neck and beard impetigo. These skin diseases were not present in asymptomatic male homosexual control subjects, 32% of whom were found to have antibodies to human T cell lymphotropic virus type III (HTLV‐III). We regard these dermatoses as early warning signs of AID

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb02845.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYThein vitroproperties of cells cultured from the dermal papilla of human hair follicles were studied and compared with those of lines of dermal fibroblasts derived from the same material. In serial subcultures, the dermal papilla cells displayed a spread out, polygonal cellular morphology at stationary growth phases and a tendency to form multi‐layered aggregates before reaching confluence. Aggregation was particularly marked when papilla cells were grown on collagen gels. In contrast, dermal fibroblasts grew as branching, parallel arrays of spindle‐shaped cells which remained as monolayers until confluence. Compared with dermal fibroblasts, papilla cells also exhibited a shorterin vitrosurvival time. The properties of cultured human papilla cells are similar to those of rat vibrissa papilla ce

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb02846.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYWe performed morphological studies on the epidermal peridermal granules (PG) of newborn mice using histological, fluorescent and electron microscopic methods. PG appeared during the 17th to 18th day of foetal life, and remained in the outermost epidermal layers until 5 days after birth. PG were evenly distributed over the entire cutaneous surface of the mouse, except the tail. PG were found to contain protein and corresponded to sites of transglutaminase activity, but they were devoid of lipids and nucleic acids. They could be differentiated clearly from keratohyalin granules. Together, these studies suggest that PG represent a unique epidermal protein product that serves as a marker of late foetal development.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb02847.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYSweat glands from normal skin obtained at autopsy or as routine biopsies were examined using a panel of immunoperoxidase‐linked antibodies. The results indicate that such a panel of antibodies defines all known functional regions of the eccrine sweat gland and provides a reliable distinction from all other skin element

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb02848.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYLectin‐binding sites in sections of formalin‐fixed, paraffin‐embedded eccrine sweat gland tumours were investigated using fluorescein isothiocyanate (FITC) conjugated peanut agglutinin (PNA), FITC conjugatedRicinus communis1 agglutinin (RCA‐1) and FITC conjugated wheat germ agglutinin (WGA). In 22 benign eccrine sweat gland tumours, lectin‐binding sites were noted primarily on the cell surface, and in the secretions. In five malignant sweat gland tumours, all showed cytoplasmic lectin‐binding sites in variable proportions of malignant cells in addition to cell surface staining. These results indicate that cytoplasmic lectin‐binding sites may be a useful marker of neoplastic transformation of eccrine sweat

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb02849.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYThe distribution of gamma‐glutamyl transpeptidase (GGT) activity was studied histochemically in benign and malignant epithelial tumours of human skin. We found that GGT activity in normal skin was confined to the secretory portion of the eccrine and apocrine glands and to the inner root sheath of the hair follicles. In Bowen's disease and actinic keratosis, GGT activity was noted focally in areas where atypical cells were observed. In extramammary Paget's disease, GGT activity was found only in large round cells scattered among GGT negative epidermal cells. No GGT activity was observed in basal cell epitheliomas or benign epithelial tumours, while squamous cell carcinoma and eccrine porocarcinoma exhibited intense GGT activity. Our study suggests that GGT may be useful as a histochemical marker for distinguishing malignant tumours from benign epithelial tumours in human ski

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb02850.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYBiopsy specimens from 185 patients with 52 different skin disorders were investigated by indirect immunofluorescence staining for the presence of HLA‐DR bearing keratinocytes and their association with an underlying inflammatory infiltrate and in particular with activated (HLA‐DR‐positive, Leu‐4‐positive) T lymphocytes. HLA‐DR expression on keratinocytes was demonstrated in 38 dermatoses, including lymphocytic vasculitis, lupus erythematosus, morphea, vitiligo, lichen planus, cutaneous T‐cell lymphoma, various infectious dermatoses, allergic contact dermatitis, granulomatous dermatoses, Sweet's syndrome, lichen sclerosus and erythema nodosum. In 27 of these this had not previously been reported. Occurrence of HLA‐DR on keratinocytes was invariably linked to the presence of a lymphocytic infiltrate containing numerous activated T‐cells (Leu‐4+, HLA‐DR+) whereas such infiltrates were not accompanied by HLA‐DR expression on keratinocytes in all the dermatoses investigated, as in pseudolymphoma and erythema anulare centrifugum. However, HLA‐DR positive keratinocytes were consistently absent in skin disorders lacking any significant lymphocytic infiltration (e.g. leukocytoclastic vasculitis, bullous autoimmune dermatoses, genodermatoses and mastocytosis). Although it has been suggested that HLA‐DR‐positive keratinocytes are involved in various immune responses of the skin, their exact functional sig

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1986.tb02851.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY