摘要:

SUMMARYAt concentrations of 10 μg/ml and greater, dithranol (anthralin) caused an intense, dose‐related activation of luminol‐enhanced chemiluminescence (LECL) of human neutropils and also increased oxygen consumption by these cells. Activation of LECL, which was maximal with 40μg/ml dithranol, occurred promptly, peaked at 3–5 min, then declined. Dithranol‐mediated stimulation of LECL was inhibited by catalase and by the protein kinase C inhibitor, H‐7. Neutrophils from individuals with chronic granulomatous disease were unresponsive to the pro‐oxidative effects of dithranol. At concentrations of 2.5 μg/ml and less, dithranol did not directly activate the LECL responses of neutrophils. However pre‐treatment of neutrophils with dithranol at concentrations of 0.5–2.5 μg/ml increased the LECL‐responses of cells subsequently two distinct dose‐related, pro‐oxidative interactions of dithranol with human neutrophils: low‐dose priming and high‐dose activation of oxidant generation. Since phagocyte‐derived reactive oxidants are immunosuppressive, these pro‐oxidative interactions of dithranol with human neutrophils may contribute to the pharmacothe

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01393.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb05917.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThere is a considerable need for reliable methods for enumeration and enrichment of Langerhans cells (LCs), since they continue to be the subject of intensive investigation in normal and diseased skin. It has been claimed that standard labelling with either anti‐HLA‐DR or OKT6 antibodies alone may fail to identify potentially important subsets of LCs with the phenotypes HLA‐DR+CD1−and HLA‐DR−CD1+. We report here on flow cytometric analysis of suction blister‐derived normal epidermal cell (EC) suspensions, double stained with phycoerythrin‐conjugated anti‐HLA‐DR and fluoresceinated OKT6. In seven separate experiments, no evidence for the existence of either HLA‐DR+CD1−or HLA‐DR−CD1+ECs was obtained. We found that HLA‐DR+CD1+LCs, which constituted a mean of 2.5% (±0.3 SEM) of all ECs, could be readily identified on the basis of fluorescence, and that their light scatter characteristics were those of moderately sized cells of low granularity. We further describe our method for flow cytometric enrichment of such HLA‐DR+CD1+LCs for functional studies, based on selection on both fluorescence and light scatter criteria. Enrichment is to greater than 90% purity, and the method is applicable to the small number of ECs (approximately 1 × 106

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01394.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb05918.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYEighteen patients with lepromatous leprosy (LL) showed a significant reduction (P<0.001) of Langerhans cells (LC) irrespective of whether the biopsies were obtained from involved (398±186) or healthy skin (304±98). The cells showed morphological changes consisting mainly of loss of dendritic processes. Twenty‐four controls (age, sex and race matched) had a mean number of LC of 632±138. In tuberculoid patients (TT) significant differences were observed, depending on the site of biopsy. Nine biopsies from involved skin had 993±206 LC, whereas 11 from healthy skin had 448±96 (P<0.001). This difference was confirmed in six additional borderline tuberculoid (BT) and TT patients in whom biopsies were simultaneouslyy obtained from involved (973±179) and uninvolved skin (498±99). In 10 patients with indeterminate leprosy the LC density did not differ from the control population (630±261). The expression of LC numbers in BT and TT patients may represent migration of these cells from healthy skin to involved areas or mobilization of a central pool. The low density found in LL patients could interfere with adequate presentation of mycobacterial antigens leading to tolerance. Alternatively the presence of T helper cells in TT infiltrates may produce factors that recruit LC; their absence in LL lesions may account for the decrease in LC e

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01395.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb05919.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb05921.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb05923.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb05924.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb05925.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY