摘要:

SUMMARYLight and electron microscopic studies have been carried out on three pigmented basal cell epitheliomas and on cell cultures of two of these tumours, to determine the formation and transfer of pigment and the fine structure of the constituent cells. Scattered in the epithelial tumour buds were many functional melanocytes that contained melanosomes in various stages of development and whose morphology was essentially normal. Many melanophages were present in the dermal stroma, but few transferred melanosomes were to be found in the epithelial tumour cells.Tissue culture studies revealed that melanocytes were present among the proliferating islands of epithelial cells and these contained many melanosomes. However, few melanosomes were found in the epithelial cells. This study indicated a block in the transfer of pigment from the melanocyte to the basal epithelioma cells.Several of the cultured epithelial cells were found to contain dense bundles of filaments resembling amyloid. It is likely that they are abnormal tonofilaments resulting from the differentiation and keratinization of basal cellsin vitro.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb06509.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYThe natural pigmentary demarcation boundaries of the skin can be classified as: Group A—lines along the upper limb with variable trans‐pectoral extension; Group B—lines along the lower limb; Group C—paired lines in median or paramedian course on the chest with midline abdominal extension; Group D—posteromedian demarcation; and Group E—bilaterally symmetrical, obliquely oriented hypopigmented macules on the chest. Groups A and C are the most distinct and occur in both Negroes a

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb06510.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARY8‐(Methylthio)cyclic AMP‐35S was applied topically to the intact or abraded skin of dogs at a dose of about 2.5 mg/kg. In this species, an average of 7% of the dose was absorbed through the intact and 62% through abraded skin. Half‐lives for radioactivity in plasma averaged 3.6 h. After the topical application of the drug to the intact skin of dogs, the average concentration in plasma of glucose, but not of insulin, was increased by as much as 21% after application to abraded skin, glucose and insulin were increased, on average, by as much as 84 and 221%, respectively. 8‐(methylthio)adenosine and two unidentified compounds were present as metabolites in the urine of dogs; some unchanged 8‐(methylthio)cyclic AMP‐35S was also excreted. 8‐(methylthio)cyclic AMP‐35S was also applied topically (25 mg; 0.32 mg/kg) to the intact and stripped skin of normal human subjects under conditions similar to those used for dogs. Three subjects with intact skin did not absorb any of the drug, whereas three subjects with stripped skin absorbed 0.5, 8.3, and 23.3% of the dose. The half‐life of radioactivity in the plasma of the subject with the greatest absorption was 0.5 h during the first 2 h, and 11 h for the next 10 h. During the first 2 h, this same subject excreted unchanged drug and 8‐(methylthio)adenosine in his urine. No changes in glucose or insulin concentrations in plasma were observed in any of the subjects nor was there any apparent irr

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb06511.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYFive distinct ester hydrolases (EC 3.1) have been characterized in guinea‐pig epidermis. These are carboxylic esterase, acid phosphatase, pyrophosphatase, and arylsulphatases A and B. Their properties are consistent with those of lysosomal enzyme

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb06512.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYInfection by Candida Albicans was found in all biopsies taken from ten patients presenting with midline lesions of the tongue which clinically presented as median rhomboid glossitis.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb06513.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYA case of plane xanthomatosis associated with multiple myeloma and hyperlipoproteinaemia in a 62‐year‐old woman is reported. The patient had IgG type lambda paraproteinaemia and type II A hyperlipoproteinaemia.The IgG paraprotein showed binding activity against high‐density lipoproteins. It could not be demonstrated that these paraprotein‐lipoprotein complexes were responsible for the hyperlipidaemia.Microchemical analysis of pathological skin demonstrated a high content of triglycerides, similar to that in eruptive xanthomata. No correlation was found between the lipid composition of the xanthomatous skin and the serum lipid composition.The results obtained with anin vitroculture method showed that the paraprotein present in the patient's serum was synthesized in the bone marrow but not in the normal or pathologic

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb06514.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYEvidence is presented that dermo—epidermal adhesion is low in mouse ear. Consequently the pressure caused by mitotic activity in the basal epidermal layer is readily relieved by cell extrusion distally and the number of basal cells per unit skin area remains low. The rule then is that the number of distal cells per unit skin area must be equally low, and the epidermis is therefore thin.Conversely, dermo—epidermal adhesion is high in mouse sole‐of‐foot epidermis, and the mitotic pressure leads through cell crowding to a columnar basal epithelium. Furthermore, in the footpad centre, where adhesion is evidently highest, the baseline folds. With basal cell crowding and baseline folding there are more basal cells, and therefore more distal cells, per unit skin area. The epidermis is thick.Epidermal cells are regarded as equipotential. The different epidermal characteristics of the different body regions are determined by the combined actions of the local mitotic rate and the local strength of the derm

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb06515.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYA method for preparing isolated epidermal cells using trypsin is described. The isolated cells retain the ability to bind antibody from patients with pemphigus or with pemphigoid in a disease‐specific manne

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb06516.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYSkin lesions from six patients with systemic lupus erythematosus, five patients with discoid lupus erythematosus, twelve patients with systemic sclerosis, five patients with localized morphoea and twenty controls were examined by immunohistological techniques using fluorescein‐labelled antihuman IgG, anti‐human C3 and anti‐human renal glomerulus antisera.The major immunohistological changes in systemic sclerosis and in localized morphoea consisted of foci of intercollagenous staining for connective tissue antigens in the reticular layer of the dermis. It is suggested that these findings indicate collagen neogenesis.In lupus erythematosus the major changes occur in the dermo‐epidermal junction and consist of deposits of IgG and C3 and thickening and disruption of the membrane as demonstrated by the use of heterologous sera containing antibasement membrane antibodies.Immunohistological techniques are useful in the diagnostic differentiation between scleroderma and lupus erythe

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb06517.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

SUMMARYA clinical evaluation of the intradermal DNA‐test was carried out on a series of patients with untreated or with treated definite systemic lupus erythematosus (SLE), or with suspected SLE with or without circulating antinuclear factors. A saline solution of a commercially available DNA‐preparation was used. The course of the DNA reaction was followed for 24–48 h after the injection.All nine cases of untreated definite SLE had a positive DNA test 6 h after the injection, and eight cases a positive result at 24 h. All seven patients with definite SLE who were on low‐dosage systemic steroid treatment had a clinically positive DNA test at 6 h. In all except one of these cases the test was still positive at 24 h. All five patients with definite SLE on antimalarial treatment had a positive test at 6 h which had become negative at 24 h after the injection. Eleven of the twelve patients with suspected SLE and circulating antinuclear factors had a positive DNA test at 6 h, which in nine cases persisted for 24 h. On the other hand, of the eleven ANF negative patients with various connective tissue diseases five had a positive test at 6 h. In only one of these cases it persisted for 24 h. Two of the eighteen control patients with various dermatoses exhibited a positive test at 6 h, and in one of these the positive reaction persisted for 24 h.It is concluded that the intradermal skin test using native DNA is a useful diagnostic tool for the detection of SLE when the reaction is followed up for 24 h. Antimalarial treatment seems to decrease skin reactivity to nat

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1975.tb06518.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY