摘要:

SUMMARYThe Tzanck test and electron microscopy with the technique of colloidal gold labelling in varicella‐zoster virus (VZV) infections were compared with virus isolation in 54 patients with clinically suspected varicella or herpes zoster infection. The Tzanck test and direct electron microscopy can determine whether or not an eruption is herpetic but cannot distinguish between herpes simplex virus (HSV) and VZV infection. However, colloidal gold immuno‐electron microscopy, using monoclonal antibodies against HSV and anti‐VZV IgG, can distinguish between these two herpes viruses. This achieves the same specificity as virus isolation followed by virus neutralization or virus typing using immunofluorescence techniques. The Tzanck test was positive in 91%, virus isolation, under optimal conditions of sampling and transportation, in 80%, direct electron microscopy (negative staining) in 80%, and colloidal gold immuno‐electron microscopy after a virus concentration procedure in 95% of the cases. The colloidal gold technique offers a rapid diagnosis in patients with suspected VZV in

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01421.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThis study describes the use of A‐scan ultrasound for the measurement and characterization of tumour tissue in small skin tumours—basal cell carcinoma, melanocytic naevus, hypertrophic scars and intraepidermal epithelioma. Assessment of the A‐scan traces by measurement of: (i) the amplitude of echoes within the area of interest; (ii) the density (number per unit depth) of these echoes; (iii) the regularity of spacing and amplitude of the echoes, and (iv) the amplitude of echoes beneath the area of interest, was used to assist in the differential diagnosis. The results show significant differences in echo amplitude between all the tumours and normal skin. When the tumour A‐scan traces were analysed the results indicated that the best discriminating feature between tumours was that of amplitude, followed by regularity, density, non‐normalized thickness and finally amplitude of echoes from beneath the tumour.Ultrasound‐derived skin thickness measurements were compared to histological measurements for the lesions, and an excellent correlation was found (r=0.96). It was considered that the A‐scan ultrasound investigation of small tumours of the skin was able to provide information on the nature of the tissues contained and may assist in their different

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01422.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe location of in vivo bound immunoreactants was studied in 37 patients with subepidermal blistering diseases by direct immunofluorescence (IMF) on suction blisters taken from uninvolved forearm skin. The patients studied included 18 with bullous pemphigoid (BP), nine with cicatricial pemphigoid (CP), three with acquired epidermolysis bullosa (EBA) and 7 hybrid cases. The patterns of IMF in the suction blisters were: BP, epidermal 1, dermal 1, combined 4, negative 12; CP, epidermal 1, dermal 2, negative 6; EBA, dermal 2, negative 1; and ‘hybrid’ patients, epidermal 3, negative 4. The different patterns of suction blister staining could not be correlated with the clinical features of the patients in respect of mucous membrane involvement, scars or milia or a history of skin fragility. Both BP and CP are probably heterogeneous in respect of their antigen specificity, and the two diseases cannot reliably be distinguished by the patterns of direct IMF on suction blisters. In additon, some individual patients with BP have more than one target antigen as indicated by a combined pattern of suction blister fluorescence. The lack of correlation between the pattern of suction blister fluorescence and the clinical features suggests that factors other than antigen specificity determine the clinical expression of subepidermal blistering disea

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01423.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYLittle is known about the molecular mechanisms or inflammatory mediators involved in delayed pressure urticaria (DPU). Pressure sufficient to provoke lesions was applied to the back of six patients with DPU. The levels of products of arachidonic acid transformation in skin exudate from the pressure challenged skin were estimated immediately after pressure was removed and 6 h later when lesions were present. These were compared to levels estimated in a similar way from unchallenged skin in these patients. Levels of leukotriene C4/D4/E4, prostaglandin E2, 12‐hydroxyeicosatetraenoic acid and leukotriene B4were not raised in lesional skin. Our results suggest that arachidonic acid metabolism is not stimulated in DP

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01424.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYIn order to investigate a possible seasonal variation in the skin response to irritants, 17 healthy volunteers were patch tested with sodium lauryl sulphate (SLS) and nonanoic acid in the winter and summer. The response to these irritants was quantified by visual scoring, measurement of transepidermal water loss, measurement of electrical conductance indicating the hydration state of the superficial epidermis, measurement of blood flow by laser Doppler and measurement of oedema by ultrasound A‐scan. Significantly stronger reactions to SLS were found during the winter than the summer as indicated by visual scoring and by measurements of transepidermal water loss, whereas no significant seasonal variation was found in the response to nonanoic acid. A decreased hydration state of the epidermis of unexposed skin was found during the winter, and we believe this to be responsible for the increased susceptibility to SLS during this seaso

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01425.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYA new polymorphism of the complement factor C3 in human plasma was demonstrated by isotachophoresis in agarose gels followed by immunodetection with rabbit anti‐human C3cand C3dimmunoglobulins. Four bands were detected in the immunoprint of freshly drawn EDTA‐plasma, which were C3s1, C3s2, C3f1and C3f2. At least four additional C3 components in Mg2+‐zymosan activated plasma were present, which were C3b1to C3b4. The different forms of C3 in frozen and thawed heparin‐plasma from 20 patients with psoriasis and 20 healthy individuals were studied from the immunoprint. The total content of C3 components was 29% greater in the patients with psoriasis than controls. The major difference was in the C3bcomponents which were increased by 46%. In psoriatic patients, the two slow C3 components C3s1and C3s2were increased by 24 and 56% respectively, when compared with controls. The two fast C3 components C3f1and C3f2were decreased to 29 and 37%. The results suggest a direct involvement of the complement factor C3 in ps

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01426.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe bacterial flora of chronic venous ulcers treated with an occlusive hydrocolloid dressing were studied over a period of 8 weeks. A novel exudate sampling method was used in an attempt to isolate anaerobic bacteria. The flora was generally stable. Once a species was present, it remained with the exception ofPseudomonas, which appeared to be inhibited by the dressing. Twelve out of 20 ulcers contained anaerobic bacteria and healing did not appear to be impaired by the presence of any particular species of bacteria.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01427.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYA continuous wave dye laser emitting light at 577 nm has been used in the treatment of 100 patients with port wine stains. Subjective assessments in 92 patients revealed an excellent or good result in 63% and a fair result in 17%. There was a 5% incidence of hypertrophic scarring and a 5% incidence of post‐inflammatory hyperpigmentation. Overall, 12% of patients experienced a poor result. The best results were obtained in older patients with purple port wine stain

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01428.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYFifty patients with seborrhoeic dermatitis were treated with 2% ketoconazole cream (n=24) or with 1% hydrocortisone cream (n=26) for 4 weeks in a double‐blind comparative study. These twice daily applications resulted in 87.2% symptomatic improvement for hydrocortisone vs. 81.6% for ketoconazole. The number ofP. ovaleyeasts was significantly reduced after the application of ketoconazole compared with hydrocortisone. The incidence of side‐effects was low in both gro

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01429.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYA modified behavioural method called habit‐reversal, in combination with potent and weak corticosteroid cream, was compared with the use of the creams alone in the treatment of 45 patients with atopic dermatitis. The patients were randomly assigned to four groups, which received two different cream regimes in combination with the habit‐reversal treatment. The patients' skin was assessed before, during and after treatment, and they recorded the amount of scratching during the study. The skin condition improved in all groups, but to a significantly greater degree in the habit‐reversal groups. A strong correlation was found between the reduction in scratching and the improvement in skin s

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb01430.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY