摘要:

SummaryThe clinicopathological features of the scarring alopecia of discoid lupus erythematosus (DLE) were studied. Scarring alopecia was present in 34% of 89 patients with DLE and was associated with a prolonged disease course. More than half these patients had scalp involvement at the onset of the disease. There was a significant reduction in size of sebaceous glands in affected scalp. Perifollicular lymphocytic inflammation was maximal around the mid‐follicle at the level of the sebaceous gland, which seems to be an important functional level in the follicle. There are changes in the expression of the matrix molecules, the proteoglycans, in the connective tissue sheath and the keratin intermediate filaments in the outer root sheath cells at this level in normal scalp and in diseased scalp. Loss of a population of mid‐follicular stem cells may be important in the pathogenesis of scarring alopecia in

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00670.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryTo investigate whether erythemal responses to ultraviolet radiation alter with age, we have reviewed the results of monochromator phototesting in adults and children, and have measured the dose–response curves for UVB erythema in a further 38 subjects. There was no significant difference between adults and children in minimal erythema dose (MED) at 300 nm; the median MED in 254 adults was 34 mJ/cm2(range 14–80 mJ/cm2) and in 24 children aged less than 15 years was 30 mJ/cm2(range 10–80 mJ/cm2). Objective measurements of UVB‐induced erythema were performed in 15 subjects aged below 25 years and 23 subjects aged above 60 years. A dose–response curve for UVB erythema was constructed for each subject and the slope of the steepest part of each curve calculated by logit regression; the values in the young subjects were greater than in the older group (P<0.02). However, there was no difference between the two groups in either the visually assessed MED or the calculated UVB dose required to produce a constant degree of mild

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00671.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe My7 antigen that is expressed on the basal cells in normal skin and inflammatory disorders is absent in the lesions of cutaneous T‐cell lymphoma (CTCL). Induction of My7 expression in basal cells in the cutaneous lesions of 12 patients with mycosis fungoides and three with Sézary syndrome treated with interferon‐α2a (IFN‐α2a) for 10 months was investigated. Biopsies were taken from the same area before treatment and after 2, 6 and 10 months of therapy with IFN‐α2a. My7 antigen was expressed on the basal cells only in those patients who showed either a complete or partial response to therapy w

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00672.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe effect of HuIFN‐βon the invasive potential of melanoma cells was studied using anin‐vitromodel system with Transwell chambers equipped with matrigel‐coated polycarbonate filters. When (102, 103and 104IU/ml) for 3 days and then grown in medium without HuIFN‐βfor another 7 days. On day 7, the proliferation of melanoma cells was inhibited by 77 and 87%, respectively, when cells were treated with 102and 104IU/ml of HuIFN‐β. This antiproliferative effect was dose‐dependent and more pronounced on day 11.The effect of HuIFN‐βon the invasive potential of melanoma cells was studied using anin‐vitromodel system with Transwell chambers equipped with Matrigel‐coated polycarbonate filters. When cells were treated with HuIFN‐β(102, 103and 104IU/ml) for 24 h, the amount of tritiated thymidine incorporated into cells was increased, indicating that cell growth was not inhibited. However, the number of cells that invaded to the filter decreased significantly by 15–40%. HuIFN‐βdid not have an inhibitory effect on the haptotactic migration of melanoma cells. These data indicate that the antiproliferative effect of HuIFN‐βoccurs after 24 h and that the direct anti‐invasive effect is independ

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00673.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryIt has been shown that tumour necrosis factor‐α (TNF‐α) induces the gene expression of collagenase and enhances the invasiveness of many cell types. However, we have previously demonstrated that interferon‐γ (IFN‐γ) induces the chemotactic response of cells and we have studied thein vitroeffects of both cytokines on invasive migration using a human fibrosarcoma cell line (HT‐1080). Invasive migration occurred with HT‐1800 cells through a basement membrane equivalent (matrigel) and collagen type I gel. Pre‐incubation of cells with increasing concentration of IFN‐γ resulted in a dose‐dependent reduction of this invasive migration. TNF‐α considerably enhanced the invasiveness of HT‐1080 cells and of fibroblasts. This effect could be significantly diminished by the pre‐incubation of cells with IFN‐γ. Inhibition of invasiveness did not appear to be due to an altered binding to the barriers or altered collagenolytic activity of these cells, as shown by attachment and collagenase assays. These data support the concept that IFN‐γ can reduce the invasiveness of transformed cells which contributes

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00674.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryLamellar body secretion results in the delivery of a selected array of hydrolytic enzymes to the extracellular domains of stratum corneum (SC). Deposition and activation of these enzymes in the interstices presumably is associated with the transformation of lamellar body‐derived lipids from a relatively polar to a non‐polar mixture, as well as the degradation of other non‐lipid intercellular substrates. To determine whether abnormal desquamation might result from failure of hydrolytic enzyme delivery to the SC interstices, we localized one catabolic enzyme, acid lipase, previously shown to be a reproducible marker for the lamellar body secretory system, by cytochemical methods within the epidermis of selected human (congenital ichthyosiform erythroderma, CIE) and animal (essential fatty‐acid deficient (EFAD) mouse epidermis and mouse tail epidermis) models associated with abnormal scaling or unusual SC retention. In addition, we compared the persistence of desmosomes within normal SC vs. the various models. Normal human and murine epidermis displayed abundant lipase activity both in lamellar bodies (LB) and in association with secreted lamellar body contents in the SC interstices. Despite normal quantities of LB in CIE, EFAD, and mouse tail epidermis, lipase activity was markedly deficient both in LB and in the SC intercellular domains. These studies support the hypothesis that normal desquamation is mediated by enzymatic modulations in lipid and/or protein content of the SC interstices, and that some forms of pathological or excessive scaling may be due to desmosomal persistence that results from defective or limited delivery of lamellar body‐derived, hydrolytic enzymes to the SC intercellul

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00675.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThe local sweating response to thermal stress (mean ambient temperature 33°C) was assessed under resting conditions on the non‐eczematous back skin of 26 young men with atopic dermatitis (AD) and in 22 non‐atopic controls with other dermatoses. The baseline (transepidermal) water loss was separately determined at room temperature (mean 23.6°C) to calculate the pure sweat loss. A gravimetric collecting method was used for the measurements at 40, 60 and 80 min. In the heated room the sweat loss in AD patients was significantly lower at all time intervals. The cumulative sweat loss was 50–60% lower in AD patients than in the controls (P<0.02). Subjects with dry AD skin had a lower sweat loss than subjects with normal‐looking skin. Compared with controls the sweat loss in AD patients was lowest at 40 min, suggesting a retarded sweating response. Half of the patients with AD and half of the controls had active participation in sports, and showed a greater sweat loss compared to the non‐sporting subjects in the

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00676.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryAtopic dermatitis (AD) is characterized by alterations in cellular and humoral immunity including elevated serum levels of IgE, IL‐2 receptor (IL‐2R) and eosinophil cationic protein (ECP). In order to evaluate the relevance of these serum parameters as indicators of disease activity, the concentrations of IgE, IL‐2R and ECP were measured in serum samples of patients with an acute exacerbation of AD (n=19) on admission to hospital and every 6 days up to discharge, and compared with those from normal non‐atopic controls (n= 15). The severity of the disease in the AD patients was examined using an established clinical scoring system. On admission, AD patients showed significantly elevated serum levels of IgE, IL‐2R and ECP compared with normal controls (P≤0.0001). Clinical improvement was associated with a decrease of both the clinical score (P≤0.001) and serum ECP levels (P≤0.005). No significant changes in serum IgE and serum IL‐2R were observed. In addition, there was a significant correlation between serum ECP and the clinical score (R=0.67,P≤0.001). These data indicate that serum ECP may be a helpful tool for monitoring dis

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00677.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryChanges in epidermal immunocytes and cytokeratins were investigated during treatment of psoriasis with calcipotriol and betamethasone valerate. Skin biopsies were obtained from 10 subjects on each treatment from lesional and non‐lesional skin at baseline, and from treated lesions after 4 weeks. In each subject, changes in expression of cytokeratins K5, K10 and K16, and changes in epidermal immunocyte counts were assessed. Responses were compared with a separate histological parameter of improvement, epidermal thickness.Both treatments produced a marked normalization of cytokeratins. The reduction of K16 expression was similar on each treatment and correlated significantly with reduction in epidermal thickness. Expression of both K5 and K10 improved less than thickness with betamethasone valerate but more than thickness with calcipotriol, although these differences did not reach statistical significance. With calcipotriol there was an increase in K5 and K10 responses with increasing response of epidermal thickness, which was not seen with betamethasone valerate.T6+cells, HLA‐DR+dendritic cells and T lymphocytes were all reduced by betamethasone valerate. There was a remarkable similarity in the level of normalization between cell types and also between cellular response and reduction in thickness. Calcipotriol produced a similar consistent reduction in cell numbers and in thickness, with the exception of T6+cells which increased in some subjects during treatment. Only in subjects in whom thickness had virtually returned to normal was there a marked decrease in T6+ce

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00678.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

SummaryThirty patients commencing isotretinoin for acne were entered into a double‐blind, randomized, placebo‐controlled trial to investigate the effect of pulsed intra‐nasal mupirocin ointment onStaphylococcus aureuscolonization and isotretinoin‐related side‐effects. In both mupirocin and placebo groups there was an increase in isolation ofS. aureusthroughout the period of treatment with isotretinoin from the anterior nares, facial skin and lips. However, these increases were significantly less in the mupirocin‐treated group. A high proportion of all patients suffered inflammatory side‐effects of isotretinoin such as cheilitis and nasal vestibulitis, with their maximum severities being recorded 2 months after starting isotretinoin. In spite of the smaller increase inS. aureuscolonization in the mupirocin‐treated group no difference was demonstrated in either the incidence of specificS. aureusinfections (e.g. furunculosis) or the prevalence of isotretinoin‐related inflammatory side‐effects. Furthermore, no relationship between the presence ofS. aureusand the severity of inflammatory side‐effects was shown.Streptococcusspecies were isolated on four separate occasions from four different patients during the study but their pathogenicity was unclear. These findings suggest that although pulsed intra‐nasal mupirocin produces a significant reduction in isotretinoin‐related staphylococcal colonization, its routine use cannot be justified on the

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1992.tb00679.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY