摘要:

SUMMARYSheets of stratum granulosum of glycerinated cow hoof epidermis showed immediate contraction on exposure to ATP in the presence of calcium. Other layers of the epidermis were unresponsive. Contraction of the stratum granulosum could be induced also by dopamine, but not by a variety of other pharmacological agents. It could be blocked by EGTA, lidocaine, or high concentrations of potassium. It is postulated that the keratohyalin granule is a contractile protein uniquely responsible for the changes observed.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1980.tb05682.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SUMMARYIn polyacrylamide gel gradient electrophoresis, serum and blister fluid of patients with bullous pemphigoid showed a nearly identical protein distribution pattern except for one more concentrated fraction in the blister fluid. This fraction could be isolated by several gel chromatographic steps and then be characterized as a protein with a molecular weight of 240,000 (BFP 240,000) consisting of several subunits. Two of these subunits could be identified as C3 fragments and two other ones as basement membrane zone antigens (BMZ‐Ag) by means of a modified Laurell technique. The molecular weight of the BFP 240,000 may be small enough to allow it to penetrate through the vessel wall into the blood circulation where, with the BMZ‐antibodies (BMZ‐Ab), BMZ‐Ag‐BMZ‐Ab complexes can be formed. The occurrence of such immune complexes in the serum has been shown in a pre

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1980.tb05683.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SUMMARYCell‐mediated immunity using the E‐rosette technique, the graft‐versus‐host reaction and intradermal skin tests were investigated in two groups of patients, twenty‐two with benign lymphoproliferative diseases of the skin (lymphocytic infiltration of the skin and lymphadenosis benigna cutis) and nine with malignant lymphoproliferative diseases (mycosis fungoides and lymphoma cutis). The effect of thymic hormone upon T lymphocytes which proved to have impaired functional capacity was investigated. Seventeen of the twenty‐two patients with benign lymphoproliferative diseases were found to have intact cellular immunity whereas seven of the nine patients with malignant disease proved to be immunodeficient. The determination of immune competence may be of help in establishing the correct diagnosis in doubtful cases and might also be useful in the follow‐up of these patients. The improvement in functional capacity of T lymphocytes resulting from their interaction with a thymic hormone indicates that it holds therap

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1980.tb05684.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SUMMARYA method is described which allows the embedding of 6 mm cutaneous punch biopsies for subsequent light and electron microscopic examination. Sections of 20 mm2cut at 0.5–1 μ are stained for light microscopy. The subtle tinctorial affinities, which approach those achieved on haematological smears, and the preservation of cellular detail frequently make subsequent ultrastructural examination unnecessary. However, when required, a special technique for trimming and resectioning the tissue blocks allows a good correlation between light and electron microscopic observatio

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1980.tb05685.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SUMMARYDeposition of immunoglobulins, complement and fibrinogen on eosinophilic staining cells was investigated using direct immunofluorescence techniques. Serum factor deposition was detected on benign epidermal eosinophilic cells seen in pityriasis lichenoides et varioliformis acuta, sunburn erythema and, in addition, on subepidermal hyaline bodies in lichen planus; no such deposition occurred on neoplastic eosinophilic cells in Bowen's disease and squamous cell carcinoma. The qualitative findings of immunofluorescence microscopy seem to be different in inflammatory and malignant dermatoses.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1980.tb05686.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SUMMARYIn twenty patients with erythema multiforme we investigated circulating immune complexes and their deposition in the skin lesions. C1q‐binding activity was elevated in ten of twenty patients, and the platelet aggregation titre was high in three of twelve tested sera. Decreased levels of C3 were seen in two and of C4 in one out of eighteen patients. Direct immunofluorescence showed a deposition of C3, IgM or IgG in the blood vessel walls of the upper dermis in four of twelve patients. These findings may suggest that transient production of circulating immune complexes and their deposition play an important role in the pathogenesis of this diseas

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1980.tb05687.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SUMMARYFibronectin is an important constituent of normal human skin, mediating cell/cell and cell/fibre interactions. In affected skin in discoid and systemic LE, changes in the distribution of fibronectin in the dermo‐epidermal junction and in the papillary dermis are observed with homogenization and degenerative changes, IF negative gaps and slit formation in the dermo‐epidermal region, together with IF positive globular bodies and transport of fibronectin into the epidermis. Unaffected skin in LE demonstrates the pattern of fibronectin as found in normal human s

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1980.tb05688.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SUMMARYWe investigated sixteen patients before and after 1 month of PUVA therapy (three times a week) for circulating immune complexes (CIC) and T‐cell markers.CIC were detected by the PEG‐C4 assay, using a laser nephelometry determination of polyethylene glycol (PEG, 3.5%) precipitated endogenous C4. T cells were evaluated by E rosettes, active E rosettes, anti‐HTLA serum and ox EA γ‐rosettes. This latter method investigated mainly T cells bearing Fc receptors for IgG (Ty‐cells).After 1 month of PUVA‐therapy: (a) the cutaneous lesions markedly improved; (b) the mean percentages of E rosettes, active E rosettes, and HTLA bearing cells, depressed before treatment, returned to normal; (c) CIC were found in higher amounts; (d) T γ‐cell numbers decreased simultaneously.The improvement of T‐cell levels assessed by E rosettes, active E rosettes and HTLA values was correlated with clearing of skin lesions, as previously reported by others. However, the increase of CIC during PUVA therapy, their correlation with T γ‐cell decrease, and their possible role in the pathogenic chain of psorias

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1980.tb05689.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SUMMARYThe prevalence of histocompatibility antigen HLA‐Cw6 was 72.7% in twenty‐two patients with the guttate form and 45.9% in thirty‐seven patients with the vulgaris form of psoriasis; the difference between these figures and the prevalence (7.4%) in 462 healthy blood donors in Finland is highly significant. The previously observed excess of several HLA‐B locus genes might be secondary to their close linkage with the Cw6 gene. Hereditary factors appear more important in the guttate than in the vulgaris form of ps

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1980.tb05690.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SUMMARYThe effect of PUVA therapy for psoriasis on the microbial flora and on levels and composition of skin surface lipid has been studied in ten patients. Samples were obtained from clinically uninvolved skin from the back and the forearm. There was no consistent significant change in the viable counts of aerobic cocci or anaerobic coryneforms during therapy. A transient rise inStaphylococcus aureuscounts was thought to be due to the use of emollients rather than to an effect of PUVA. Genetic changes in the microorganisms were not sought.There was an increase in the amount of total lipid (both casual levels and replacement sums) during therapy. An increase of free fatty acids at the expense of triglycerides did not reach statistical significance.The influence of PUVA on the skin flora seems to be lessin vivothan anticipated fromin vitrostudies.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1980.tb05691.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY