摘要:

SUMMARYThe topical application of 0.1% retinoic acid (RA) on human skin over a period of 4 days, whether or not under occlusion, did not increase either IL‐iα or β immunoreactivity as determined by a sensitive enzymoimmunoassay. No down modulation was seen following the application of a potent topical corticosteroid. Occlusion increased the yield of IL‐iβ immunoreactivity. Immunoblot patterns of epidermal extracts revealed both the mature form of IL‐i(17 kDa) and the precursor (36 kDa) and were identical in amounts whether the specimens were from controls or from RA‐ or corticosteroid‐trcated skin. There was a slight modification in the pattern of high molecular weight proteins (52 kDa) probed by the anti‐IL‐iα and β sera. It appears that the IL‐1 epidermal immunoreactive pools are barely amenable to modulation because they represent a storage form linked to end‐stages of kerati

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb06286.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYTwenty patients with psoriasis were treated with the vitamin D3analogue MC903and betamethasone ointment in a double‐blind trial with a left‐right comparison. In addition to the clinical severity scores, Ks8.12 binding which detects keratin 16 expression and the DNA synthesis were quantified using flow cytometry. Both markers decreased significantly with treatment, but remained above the normal range even in those who had total clearance of the lesions. Treatment with MC903with regard to Ks8–52 binding was significantly better than with betameth

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb06287.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYThe role of locally released cytokines in inducing lymphocyte activation and infiltration in the skin lesions of mycosis fungoides has been investigated. The levels of selected cytokines were measured in chamber fluid samples from lesional and control skin. Biologically active interleukin 6 was significantly elevated in lesional samples and a recombinant form of this cytokine was shown to induce lymphocyte migration in anin vitroassay. Biologically active interleukin 1 was detected in all control chamber fluid samples. Significantly reduced levels of this cytokine were present in lesional samples, which may be the result of the release of preformed material. Interleukin 2 and tumour necrosis factor activity, and γ interferon and granulocyte macrophage colony‐stimulating factor immunoreactivity, were not detectable in any of the samples. Interleukins1and 6 may play a role in the pathogenesis of the lesional lymphocyte infiltrates in mycosis fungoid

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb06288.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYWe have studied 23 patients receiving penicillamine for the treatment of rheumatoid arthritis to determine the prevalence of penicillamine‐induced elastosis. One female patient had pseudo‐xanthoma elasticum‐like skin changes and bramble‐bush elastosis without calcification in the involved skin. Penicillamine elastosis was present in the joint capsule in 62% of eight patients or 64%, of II joints examined and was detected in joint capsules after as little as 1 year of tr

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb06289.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYKaposi's sarcoma (KS) is a low grade malignant neoplasm which shows invasive growth and often occurs in immunosuppressed patients with the Acquired Immune Deficiency Syndrome (AIDS; epidemic KS). It is also found in elderly men where it is usually limited to the skin (classic KS). The present study investigated the chemotaxis and invasive migration of epidemic KS cellsin vitroand compared them to cells grown from classic KS lesions and to fibroblasts. Epidemic KS cells demonstrated invasive migration through reconstituted basement membrane (Matrigel) as well as through interstitial connective tissue (collagen I) in early passages, whereas fibroblasts did not invade either barrier. Epidemic KS cells in late passages did not show any invasive migration. Following pretreatment with tumour necrosis factor α (TNF‐α) there was no enhanced migration through the Matrigel and collagen I for epidemic KS cells, whereas classic KS cells showed an increased migration through the type I collagen barr

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb06290.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYThe median zinc content of neutrophils was significantly reduced in 16 patients with psoriasis in comparison to both normal controls and six patients with seborrhoeic dermatitis (P<0.05). This reduction was unrelated to the extent of skin involvement. Plasma and erythrocyte zinc levels were unchanged.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb06291.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYThe autologous mixed lymphocyte reaction (auto‐MLR) was studied to test the interactions between immunocompetent cells in patients with psoriasis. The auto‐MLR in 20 patients with psoriasis was significantly lower than in 16 normal controls. Lower values were found in untreated psoriatic patients than in those in remission following treatment. The values in the latter group were significantly lower than in controls and in six patients with atopic dermatitis in remission. The tendency for an increase in the auto‐MLR with a decrease in disease activity was further confirmed in five patients studied before and after treatment. In contrast, the allogeneic lymphocyte reaction (allo‐MLR) in psoriatics was similar to that in normal c

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb06292.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYInterferon‐γ (IFN‐γ) induces the expression of MHC class II antigens on non‐lymphoid cells. In this study we investigated the effect of 1,25‐dihydroxyvitamin D3(1,25(OH)2D3), the most active metabolite of vitamin D3, on the IFN‐γ‐induced expression of MHCclass II antigens on HEp‐2 cells. We found that 1,25(OH)2D3enhanced the IFN‐γ‐induced expression of HLA‐DR on these cells. Differential effects on the MHC class II antigens HLA‐DR, ‐DQ

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb06293.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYPentoxifylline, an analogue of the methylxanthine theobromine, inhibits the proliferation and certain biosynthetic activities of fibroblasts derived from normal human skin. Fibroblasts from the skin of patients with keloids, scleroderma and morphoea were culturedin vitroin the presence and absence of pentoxifylline (100–1000 μg/ml) to determine whether it inhibits fibroblast proliferation and the production of collagen, glycosaminoglycans (GAG), fibronectin and collagenase activity. The exposure of subconfluent fibroblast cultures to pentoxifylline resulted in non‐lethal, dose‐dependent reductions in serum‐driven fibroblast proliferation, with 1000 μg/ml pentoxifylline virtually negating the proliferative effect of serum on the cells. The fibroblasts assayed as confluent cultures produced reduced amounts, by up to 95%, of collagen and GAG, dependent on the concentration of pentoxifylline, both in the presence and absence of serum. Pentoxifylline similarly inhibited the fibronectin production by keloid and scleroderma fibroblasts, but had no effect on collagenase

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb06294.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

SUMMARYThe side‐effects of long‐term cyclosporin A (CyA) treatment in 26 patients with severe psoriasis were evaluated. These patients had a mean PASI score of 30.2 and were treated with CyA for between 7 and 37 months (mean 19.5 months). There were three groups according to the dose of CyA,3 mg/kg per day. In all three groups, CyA was found to be equally effective. Treatment with CyA was discontinued in 12 of the 26 patients because of nephrotoxicity and/or development of hypertension. One was in the3 mg/kg per day group. There was no hepatotoxicity with CyA treatment. One patient developed two squamous cell carcinomas of t

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1990.tb06295.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY