摘要:

SUMMARYThe level of cleavage was determined in a variety of acquired bullous diseases of the dermal‐epidermal junction zone (bullous pemphigoid, dermatitis herpetiformis, porphyria cutanea tarda and epidermolysis bullosa acquisita). We used an indirect immunofluorescence technique to examine the basal membrane zone with anti‐type IV collagen and anti‐laminin antisera and bullous pemphigoid sera. The majority of blisters examined proved to be junctional, including those from disorders hitherto considered to be dermolytic. Dermolytic cleavage was encountered only sporadically in microvesicles of dermatitis herpetiformis, in one small vesicle and in one out of five large blisters of porphyria cutanea tarda and in a large lesion of epidermolysis bullosa acquisita. We conclude that in acquired bullous disorders of the dermal‐epidermal junction zone the preferential site of split formation is the lamina lucida which appears to act as alocus minoris resistentiae; dermolytic split formation of substantial extent occurs only when the sublaminal fibrillar apparatus is mechanically comp

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07671.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYAnti‐D47 is a monoclonal antibody reacting with a surface antigen of human cortical thymocytes (different from the T6 antigen). It was prepared by immunization of mice with human thymocytes. The known cross‐reactivity of monoclonal antibodies prepared against thymic cells with skin components prompted us to test anti‐D47 on human skin as well as animal tissues. No labelling was observed on animal tissues. On human skin, anti‐D47 was found to react with the secretory portion of eccrine sweat glands (ESG), i. e. with a cytoplasmic antigen of the secretory cells lining the deep portion of ESG glomeruli. No labelling was observed on apocrine sweat glands or on the excretory portion of ESG. Anti‐D47 was also tested on histologically proven extramammary (vulvar) Paget's disease and basal cell epitheliomas: no staining of the neoplastic cells was observed. Anti‐D47 appears to be an immunological marker of the secretory cells of human ESG and a new tool for the investigation of human sweat glan

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07672.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYMeasurements of transcutaneous voltage have been made on seventeen normal volunteers. The results show the presence of ‘skin battery’ voltages comparable in size to those previously reported for amphibian and mammalian skin. No correlation was found between battery voltage and age or sex in the group studied, but consistent anatomical variations were observed. The possible role of these voltages in the natural wound healing process is discus

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07673.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYWe have usedUlex europaeusI (UEA I) lectin, specific for α‐l‐fucose‐containing glycoconjugates, in fluorescence microscopy to stain cryostat sections of human skin from normal persons and patients with psoriasis and lichen simplex. In normal skin the upper layers of the stratum spinosum and the stratum granulosum were strongly reactive with UEA I, whereas the lower layers of the epidermis did not react. The staining intensity of the upper epidermis was similar to that of the endothelium of dermal blood vessels. Biopsies of the lesional skin of lichen simplex showed an intense UEA I‐specific staining throughout the whole epidermis, similar in intensity to that seen in the upper epidermis of normal skin.In psoriatic lesions positive UEA I‐specific fluorescence was seen throughout the whole epidermis, but the fluorescence was more faint and often granular. In uninvolved skin of psoriatic patients the whole epidermis showed a diffuse UEA I‐specific fluorescence, differing in this respect from normal skin. In normal skin UEA I binds to epidermal cells which are at a certain state of differentiation.The results with psoriatic epidermis confirm that both uninvolved and lesional epidermis have a defect in epidermal maturation, as shown by the altered binding of

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07674.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYSurface glycoproteins of cultured human keratinocytes from normal skin and uninvolved psoriatic epidermis, isolated by the suction blister method, were studied by two different methods. Cells were cultured on collagen‐coated culture dishes and showed a fibrillar keratin‐specific staining by immunofluorescence. Surface labelling experiments using the neuraminidase/galactose oxidase/sodium borohydride method (which labels the penultimate galactose moieties of glycoproteins) revealed one major glycoprotein with Mr53 kD (kilodaltons) both in normal keratinocytes and in keratinocytes from uninvolved psoriatic skin. The periodate/sodium borohydride method (which labels the terminal sialic acids in glycoproteins) by contrast revealed three major glycoproteins, with Mr53 kD to 63 kD, in normal keratinocytes but only a single major glycoprotein, with Mr53 kD, in keratinocytes from uninvolved psoriatic skin.Treatment of cultured keratinocytes with etretinate appeared to restore the normal pattern of surface glycoproteins in uninvolved psoriatic keratinocy

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07675.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYA kinetic method was used to determine the rates of phagocytosis of IgG‐coated and C3b‐IgG‐coated latex particles by neutrophil granulocytes from twenty patients with psoriasis and twenty with palmoplantar pustulosis (PPP). Patients with psoriasis showed a highly increased rate of uptake of IgG‐coated particles. An increased rate was also observed in patients with PPP. These results indicate enhanced Fc‐receptor function. In healthy controls the uptake rate of C3b‐IgG‐coated particles was at least 30% higher than that of particles coated with IgG alone. In nearly half of the patients in the psoriasis and PPP groups the uptake rate failed to increase in the normal way, indicating that C3b dysfunction is common in both psoriasis and PPP. In neither group was there any relationship between the rate of phagocytosis and the clinical severity o

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07676.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYInterferon‐like activity could be detected in the sera of nineteen psoriatic patients (interferon titres between 8 and 64 i. u./ml), whereas healthy controls were negative. Following PUVA treatment the interferon levels increased significantly (interferon titres between 32 and 128 i. u./ml). An enhanced interferon production could explain some of the immunological phenomena connected with PUVA treatmen

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07677.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYThe densities of T6 antigen‐bearing Langerhans cells in 112 biopsies of human skin from sixteen surgical out‐patients and four cadavers were determined for eight anatomical regions. The regional mean densities (± s. e. m.) of epidermal Langerhans cells per mm2were: head and neck, 489 ± 27; chest, 466 ± 22; back, 466 ± 11; upper extremities, 458 ± 25; lower extremities, 431 ± 30; buttocks, 411 ± 11; genitalia, 298 ± 45; soles, 58 ± 12. No statistically significant differences were found between any of these Langerhans cell densities except for that of the soles which was lower than those of all other regions (P<0.002). No significant differences were detected between the mean densities of patients and cadavers, Caucasians and Hispanics or males

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07678.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYPeripheral blood lymphocyte cyclic AMP responses to isoprenaline, prostaglandin E2 and histamine were examined in patients with atopic eczema, in the presence and absence of a potent phosphodiesterase inhibitor (PDEI). Basal cyclic AMP levels were not significantly different in atopic and control groups. In the presence of the PDEI, there were impaired cyclic AMP responses to both isoprenaline and prostaglandin E2 in the atopic group. Differences between atopic and control cyclic AMP responses were exaggerated by the omission of the PDEI, when impaired responses to all agonists were observed in lymphocytes from atopic subjects. These results imply that, in atopic eczema, lymphocytes exhibit impaired responses not only at the beta‐adrenoceptor site but at unrelated sites of adenylate cyclase activation, and these observations are consistent with increased leukocyte phosphodiesterase activit

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07679.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

SUMMARYA dose‐dependent increase in the mass of flank organ hair was produced in 11‐week‐old female hamsters by subcutaneously injected testosterone propionate. The mass of androgen‐stimulated flank organ hair was decreased bilaterally in a dose‐dependent manner by cyproterone acetate applied topically to one flank organ of eac

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1983.tb07680.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY