摘要:

Some technical aspects of MLC inhibition by sera obtained from multiparous women were studied. The variability of the MLC response was very high. The serum source used in the cultures was probably to a large extent responsible for this variability. Aspecific inhibition, which was observed with some test sera, could be removed by dialysis against PBS. To make the evaluation of inhibition by immune sera objective, a scoring system was introduced for the degree of inhibition. Test sera were usually added to the cultures. Alternatively, stimulator and responder cells were preincubated with the test serum. Preincubation of the stimulator cells did not show a difference in inhibition pattern when this was compared with serum addition. Preincubation of the responder cells showed a completely different inhibition pattern.The MLC inhibition test compared very well with an indirect immunofluorescence test and a cytotoxicity test using B‐cell enriched cell suspension

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01114.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

The incidence of MLC‐inhibiting antibodies was determined in 42 pregnancy sera. MLC's were carried out between the cells from the serum donor and her busband in the presence of nonimmune AB serum and the test serum. Fifty per cent of the sera reduced the MLC response to less than 40% of the control values. Only four sera had lymphocytotoxic activity. The inhibition was strong against the specific immunizor, less strong against random unrelated cells and weak against cells which were SD‐identical with the serum donor. Absorptions with lymphocytes and platelets were carried out. Lymphocytes removed activity in three of the four sera tested. Platelets removed activity from one serum. It was concluded that both anti‐LD and anti‐SD anti‐bodies were able to inhibit the MLR at the stimulator c

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01115.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

The overall error rate of the microlymphocytotoxicity test has been reduced from 1.08% in 1971 to approximately 0.35%, based on comparisons of 882 replicate test pairs (different bleedings) and on family studies, with 0.11% attributable to “reading” errors. This reduction was attributed to automation for lymphocyte adding, better incubation temperature control, better technical preparation of cells and stronger, more monospecific sera. Three types of errors contribute to the overall error rate: random, sera‐related (affected by strength and specificity), and lymphocyte‐related (possible B‐cell reactivity and donor/lymphocyte physiological condition). Strongly skewed error distributions, which seem to be lymphocyte‐related, occurred in less than 4% of the

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01116.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

A Japanese population was typed for HLA‐D antigens for the first time using HLA‐D homozygous typing cells Dw1 through Dw4 and the homozygous cells LD HO and LD AH, newly found in the Japanese population. Dw3 was absent from the Japanese population which also lacks B8. Dw1, Dw2 and Dw4 were found in this population, but these did not show significant linkage disequilibria with any alleles of the B or C loci. LD HO and LD AH were common in Japanese but absent in Caucasians. LD HO was found to be in strong linkage disequilibrium with Bw35. LD AH showed significant association with Bw

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01117.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01118.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01119.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY