摘要:

Associations between allelés at the DPA1 and DPB1 loci were analyzed in 181 Norwegian caucasoids. Associations were observed between the DPA1 *0201 and the DPB1 *0101, *0901, *1001 and *1101 alleles. An association between DPA1 *0201 and the DPB1 *0901 allele was also observed among 23 healthy Japanese and 24 Japanese MS patients. In addition, it appeared that among Japanese the DPA1 *0201 allele may be associated to the DPB1 *0501. In conclusion, alleles at the DPA1 and DPB1 loci display different associations among Norwegian caucasoids and Japanese

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01950.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:The HLA class II genotypes were determined in the B‐lymphoblastoid cell lines selected for the Tenth International Histocompatibility Workshop. The HLA class II genes were determined by the PCR‐SSOP method using the reagents provided by the Eleventh Histocompatibility Workshop. Additional studies have been performed for further characterization of HLA class II polymorphism on these cell lines. It is observed that several cell lines have HLA class II haplotypes with the same DRB1, DQA1 and DQB1 alleles on both haplotypes but different alleles at the other class II loci, confirming that these cell lines are not truly HLA class II‐homozygous. Other cell lines carried HLA class II haplotypes which were only different at the DRB1 gene. These results suggest double recombination events or gene conversion‐like events in generation of HLA DR, DQ haplotypes. These cell lines provide an important tool as references for HLA DNA

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01951.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:The canine LeuCAM (CD11/CD18) family is characterized using a panel of newly developed anti‐canine LeuCAM monoclonal antibodies (mAb) as well as a cross‐reactive anti‐human CD11b mAb. The new anti‐canine LeuCAM mAb were developed by immunizing mice with purified canine CD11/CD18 antigen derived from an anti‐canine CD18 affinity column. Six mAb with reactivity to the canine LeuCAM family were cloned and characterized. These 6 included a new anti‐canine CD18 mAb, 2 anti‐canine CD11a mAb, 2 anti‐canine CD11c mAb, and a mAb which recognizes a novel canine CD11/CD18‐related macrophage antigen, designated LeuCAMmac, which is described in a separate report. The cell and tissue distribution of canine LeuCAMs was found to be similar to that reported in other species with a few notable exceptions. One prominent exception was the finding that canine CD11c, in contrast to human CD11c, was much more widely distributed on dendritic cells than it was on tissue macrophages. The molecular organization of the canine LeuCAM family was also found to be similar to that reported in other species, with the possible exception of the canine CD11b α chain, which may exist as several different species. It is anticipated that these anticanine LeuCAM mAb will prove useful in immunophenotypic studies of canine hemolymphati

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01952.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:Eighty‐one Behcet's disease patients have been studied for HLA association by HLA‐DRB1, ‐DQA1, ‐DQB1 and ‐DPB1 genotyping with the polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) technique and for NcoI RFLP in the tumor‐necrosis factor (TNF β) gene by Southern hybridization in addition to serological typing. In serological typing, the frequency of HLA‐B51 was significantly increased in the patients. In PCR genotyping, there was a significant increase in the HLA‐DRB1*0802, DQA1*0301 and DQB1*0303 alleles, whereas the frequencies of DRB1*1502, DQA1*0103, DQAl*0101, DQB1*0601 and DQB1*0501 showed a significant decrease in the patients. No significant difference was observed in any HLA‐DPB1 alleles. Among them, B51 was found to be a genetic marker most strongly associated with Behcet's disease (p<0.00005, δ2=46.47, pc[corrected p]<0.005). The positive and negative associations of class II alleles with the disease can be explained by linkage disequilibrium with B51, and do not reach statistical significance by the corrected p‐value test. In NcoI RFLP typing in the TNFβ gene, 250 kb centromeric of the HLA‐B gene, the frequency of a 5.5 kb fragment was considerably decreased in the patients when compared to the controls, although the decrease was not statistically significant. These results indicate that the HLA class II genes or other non‐HLA genes mapping within the class II region are not involved in the development of Behcet's disease but that HLA‐B51 itself or a non‐HLA gene located around the HLA‐B gene region, more closely linked to HLA‐B than the TNFβ gene, does determine the gen

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01953.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:HLA‐DRB1 nucleotide sequence polymorphisms have been examined in 304 Melanesians from the Papua New Guinean coast (Madang), islands (Rabaul) and highlands (Goroka), and from New Caledonia and Fiji. A total of 20 HLA‐DRB1 alleles were detected by oligonucleotide hybridizations of exon 2 HLA‐DRB1 polymerase chain reaction products, in a typing protocol designed to detect all 42 officially‐designated HLA‐DRB1 alleles. DRB1*1502 and 1101 alleles were the most common alleles in coastal and island Melanesians, while DRB1*1501, 1502 and 1408 predominated in Papua New Guinean highlanders. Undefined mixed lymphocyte reaction determinants in earlier studies of Melanesians could be accounted for in the present study as DRB1*0410, 1407 and 1408 in Papua New Guinean highlanders and as DRB1*1104 and 1602 in coastal people. Nucleotide sequence polymorphisms at HLA‐DQA1, ‐DQB1, ‐DRB3 and ‐DRB5 were also determined for estimating HLA‐DR,DQ allelic disequilibrium relationships; unusual haplotypes in Melanesians included DBR1*1502, DRB5*0101 and DRB1*0410, DQB1*0402. Previous claims of limited heterogeneity in the HLA‐DR allele repertoire in Melanesians are now seen to reflect limitations of early typing reagents rather than any dramatic restriction in HLA

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01954.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01955.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01956.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01957.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01958.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01959.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY