摘要:

Cultured porcine non‐lymphoid cells, characterized by biochemical and morphological criteria, were derived from different tissues of individuals typed by serological and mixed lymphocyte culture methods for gene products of the major histocompatibility complex. These cultured cells have been used as stimulators in mixed lymphocyte‐tissue cell cultures in order to investigate (1) the magnitude, kinetics and dose‐dependence of lymphocyte transformation caused by tissue cells compared with that caused by lymphocytes as stimulators; (2) the relationship between the expression of serologically detected Ia‐like antigens by tissue cells and their ability to cause lymphocyte transformation; (3) the genetic control of stimulation by tissue cells and by lymphocytes and (4) the expression and genetic control of lymphocyte stimulatory properties restricted to tissue cells and absent from lymphocytes. It has been shown that some but not all kinds of tissue cells can stimulate allogeneic lymphocytes strongly and that the characteristics of such stimulation are similar to those observed in mixed lymphocyte cultures. Strong stimulation by tissue cells does not always correlate with the expression of serologically detectable Ia‐like antigens, but appears to be controlled by the major histocompatibility complex. There is evidence that certain tissue cells possess lymphocyte stimulatory properties not shared by lymphocytes. Preliminary data suggest that such tissue cell specific stimulation is not controlled by the major histocompatibility complex, though more detailed genetic analysis is

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00865.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00866.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

A procedure for the preparation of liposomes incorporating membrane components of human lymphoid cells is described, whereby the detergent used to solubilize the plasma membrane is almost completely eliminated. MHC products HLA—A—B—C and DR molecules were shown to be incorporated into the liposomes and their antigenic determinants to be exposed on the outer surface. A protein‐rich liposomal fraction could be separated from both protein‐free liposomes and from aggregates. This preparation may be suitable for testing the functions of cell membrane components in biological assa

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00867.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

The51Cr release assay (CRA) is the commonly accepted technique for measurement of cell mediated cytotoxicity. This assay shows some disadvantages when mononucleated cells of human peripheral blood (MNC) are used as effector and target cells. The uptake of51Cr by PHA stimulated lymphocytes is low compared to the spontaneous release. In an attempt to develop a cytotoxicity assay suitable for human lymphocytes we used14C‐TdR to label target cells surviving after contact with effector cells. Cytotoxic lymphocytes were generated by incubation of MNC with irradiated allogeneic MNC for 6 days. On day 6 the effector cells are irradiated and co‐cultured with PHA stimulated target cells. Twenty‐four hours later14C‐TdR is added. After an additional 24 h the cultures are harvested and14C‐TdR taken up by target cells is measured. It is shown that the effector cells are still cytotoxic after irradiation. These cells do not take up14C‐TdR. Cell‐free supernatants do not influence the uptake of14C‐TdR by target cells. The results obtained with this assay correlate very well with those obtained by the CRA, if the spontaneous release does

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00868.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Study of the incidence and segregation of the serologically detectable A and B products of the HLA complex in 140 family units in which one or more offspring was afflicted with a developmental craniofacial anomaly has uncovered no evidence of an association between HLA—A or B antigens or haplotypes and the malformations under study. Further analysis of HLA—D products in the same family units by the mixed leukocyte culture (MLC) technique has, however, uncovered a relatively high incidence of non‐reactivity between the cells of one (or both) parent(s) and cells of some offspring in 41 of the 140 families included in this study. The parent couples involved in this finding were unrelated and generally did not share any HLA—SD haplotypes. When this finding was studied further by Primed LD Typing techniques, the results in six families suggested that such MLC non‐reactivity is a consequence of the sharing of LD alleles by each pair of parents in these families. The known polymorphism of the HLA—D locus (or loci) and the low incidence of comparable findings in the normal population suggest that LD allele sharing in this particular population may be related to the selection of certain particular HLA—D products in families afflicted with developmental craniofacial anomalies. This result may be relevant to the possible existence in man of an analogue of the murine T/t complex which may occur in linkage with the HLA complex, in the same manner as the linkage disequilibrium which has been documented between the t complex and H‐2 in chromosome

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00869.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

The possibility of an association between HLA profile and the probability of having esophageal cancer was explored among patients from different ethnic groups residing in the Caspian Littoral of Iran. A total of 151 esophageal cancer patients and 214 normal controls with ethnic affiliations similar to those of the patients were typed for HLA antigens. No statistically significant differences were found between patients and controls with regard to HLA antigens.

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00870.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Forty‐one unrelated juvenile, insulin‐dependent diabetics have been HLA tissue typed for A, B and Dw anitgens and compared with a normal control population. We have found statistically significant increases in the frequencies of B8, B18, and Dw3, and significant decrements in the frequencies of B7, B12 and Dw2. The log‐linear modeling technique was used to study the association of JIDD with Dw3 and B8 antigens. We confirmed that the B8 excess seen in diabetics is secondary to the excess of Dw3. The decrements of B7, B12 and Dw2 could reflect an association of these antigens with a protective factor for the disease, or could be due to an artifact. The latter possibility was excluded for B7 and Dw2 by adjusting for the excess antigen frequencies.These findings suggest that the associations between the HLA and diabetes are compatible with the existence of genes which are concerned with the pathogenesis of the disease and are closely associated with the D locus of the major histocompatibility s

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00871.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Using the two color fluorescence (TCF) method, alloantibodies against subsets of T cells could be detected in sera from pregnant women with strong HLA antibodies. To preclude interference of these HLA antibodies with the recognition of the T cell antibodies, serum donors were selected which were HLA—Al, —B8, —DRw3. Their sera were tested on a panel of individuals homozygous for HLA—Al, —B8, —DRw3. By enriching peripheral mononuclear blood lymphocytes for Tγ cells it could be shown that some of the sera reacted mainly with Tγ and others with Tμ lymphocytes, while some sera rea

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00872.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00873.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Twenty‐one unrelated Caucasian patients with Primary Biliary Cirrhosis were typed for HLA—A, B, C and HLA‐DRw antigens. The antigens HLA‐A1, HLA‐B8 and HLA‐DRw3 were found in increased frequency in relation to the control group. When thePvalues were corrected for the number of antigens tested only the increase of HLA—DRw4 remained significantly different from the control group (Pcorr.<0.004). The antigen HLA—DRw3 was carried by 12 out of 21 patients (57.1%) in comparison to 11 out of 74 (14.8%) normal unrelated Cau

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00874.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY