摘要:

Two rhesus (Macaca mulatto) monkey lymphocyte‐defined (LD) antigens have been identified using two typing cells as stimulators in a one‐way mixed leukocyte culture (MLC) assay. An analysis of the genetic behavior of these LD antigens in six rhesus monkey families revealed that both antigens were linked with RhLA. One probable recombinant indicated that the LD locus lies outside the two known RhLA‐SD loci and the locus which controls the serum protein, properdin B(Bf). These two antigens, LD1 and LD2, had observed gene frequencies of 0.07 and 0.25, respectively. Neither of these two new LD antigens was significantly associated with any serologically defined (SD) an

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01143.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Interest recently has extended to the problem of looking for HLA haplotype associations with particular diseases. Higher order interactions, that is to say of particular haplotypes with a disease, are much more complicated than individual antigen associations, with which most disease studies have so far been concerned. Our aim in this work is to explain the concept of higher order interactions and to discuss the difficulties involved when trying to measure such haplotype associations. The main emphasis is to point out that it is not easily possible to detect haplotype associations if an individual antigen association with the disease exists.

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01144.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

In a search for bone marrow donors and HLA—D homozygous cells, MLC family studies were performed. Results of the experiments, in which the Seventh Workshop cells 7W569‐KR and 7W568‐WE were found, are presented here. Within the KR and the WE families two identified and two non‐identified HLA—D alleles appear to be linked to the HLA—B12 allotype that both fam

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01145.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Cytotoxic lymphocytes (CTL) can be generated in mixed lymphocyte culture and cryopreserved after a 6‐day sensitization phase. CTL tested after storage in liquid nitrogen do not show lysis of autologous target cells and can mediate strong cytotoxicity against other target cells. This lysis shows the same specificity as that of the corresponding fresh CTL. The ability to cryopreserve CTL enables performance of sequential CML testing. Data from separate CML experiments can be pooled using a percent relative cytotoxic response (% RCR) to normalize values within individual experiments. Together, these methods provide a standardized cell‐mediated lympholysis techni

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01146.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Polynesians living on the island of Mauke in the Cook Island group were typed for HLA—A and —B locus antigens. The Mauke population has restricted HLA polymorphism, with five A‐locus antigens and four B‐locus antigens accounting for a majority of the HLA phenotypes. Although some differences in antigen frequency were found when Mauke Islanders were compared with Polynesians from Easter Island and Samoa, the Mauke Islanders were closer in their HLA antigenic profile to Polynesians than to Mela

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01147.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

The sera of three children with chronic benign neutropenia, due to anti‐neutrophil antibodies, were studied with respect to their antibody specificity. This was done by screening the sera against a panel of leukocyte donors in the EDTA micro‐agglutination test and in the indirect fluorescence test. Two of the sera contained antibodies against the known neutrophil‐specific antigen NA2. The third serum was directed against a new neutrophil‐specific antigen. Genetic analysis showed no correlation between this antigen and the already known neutrophil‐specific antigens: 9A, NA1, NA2, NB1, and NC1. In the Dutch population the frequency of the new antigen, tentatively called NE1, is 23%, which gives a gene frequenc

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01148.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

In the complement dependent lymphocytotoxic microtechnique it was found that antibody‐killed frozen B lymphocytes are sufficiently stained for reliable reading after 30 min of incubation with trypan blue, while the background of staining is only about 10%. During the next 30 min of incubation, however, the background of staining increases to about 30%, whereafter it remains constant for at least 24 h. Formaldehyde is able to stop the trypan blue uptake by killed or damaged lymphocytes completely. Consequently, if formaldehyde is added to the reactions 30 min after the trypan blue addition, the otherwise rapidly increasing background of staining is kept at an acceptable level of 10%, thus making HLA—DR typing of frozen stored B lymphocytes possible. The trypan blue staining seems rather independent of incubation conditions before the addition of the dye. Similar results were obtained with T lymphocy

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01149.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

A population sample of 5,202 persons comprising normal, healthy individuals from three laboratories in Copenhagen was used for estimating HLA‐A, ‐B antigen, gene, and haplotype frequencies, besides delta‐values and their standard errors. The serum panels used allowed the determination of a total of 273HLA‐A, ‐Bhaplotypes, and the most significant Δ‐values were in agreement with those found in other Caucasian populations. The methods used for the calculations of frequencies, delta‐values, and standard errors

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01150.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

200 Greeks were typed by lymphocytotoxicity for 14 HLA‐A and 18 HLA‐B antigens with 120 sera. A high frequency of Bw35 and B5 antigen was apparent. The most remarkable disequilibria found were those between A2‐B5 and A

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01151.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb01152.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY