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1. |
HLA‐DR and ‐DQ genotyping by PCR‐SSO in Shanghai Chinese |
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Tissue Antigens,
Volume 41,
Issue 5,
1993,
Page 223-226
Fu Qing Wang,
Gilbert Semana,
Renée Fauchst,
Bernard Genetet,
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摘要:
The polymorphism of HLA class II (HLA‐DRBI, ‐DQA1 and ‐DQBI) were investigated in 89 Shanghai Han Chinese using polymerasechain reaction amplification and oligonucleotide (PCR‐SSO) typing. Of the 43 DRB1 alleles tested, 24 were observed. DRBI *0901 (16.85%), *0803 (9.55%), *1202 (9.55%) and *1501 (12.92%) were most frequent and account for the high frequencies of DR9, DR8, DR5 and DR2 specificities in this population. The alleles DRB1 *0101. *0102 and *1001 had very low frequencies. Among the 8 DQA1 and 13 DQB1 alleles tested, only DQA1 *0401 and DQB1 *0402 were absent. DQA1 *0301, and DQB1 *0301 and *0303 were among the most common alleles of the two loci respectively. Unusual DRB1‐DQA1‐DQB1 haplotypes were observed and a DRB1 “new” allel
ISSN:0001-2815
DOI:10.1111/j.1399-0039.1993.tb02010.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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2. |
The HLA polymorphsm of two distinctive South‐American Indian tribes: The Kaingang and the Guarani |
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Tissue Antigens,
Volume 41,
Issue 5,
1993,
Page 227-237
Maria Luiza,
Petzl Erler,
Roberto Luz,
Vanessa Santos Sotomaior,
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摘要:
The HLA‐A, B, C, DR and DQ antigens of 240 Kaingang and 98 Guarani individuals have been characterized. The most frequent antigens found among the Kaingang are A31, 2, 24; B35, 51, 39, 48; Cw4, 7, 3, 1; DR8, 4, 2; DQ blank, 3. In the Guarani, they are A2, 28, 31; B40, 62, “53G”; Cw3, 4; DR2, 4, 8, 6; DQ3, blank. B “53G” is an unusual antigen of the B5 cross‐reactive group. DQ blank possibly corresponds to DQ4, not tested in this study. The reaction patterns of B35, B40 and DR4 indicate intra‐tribal (of B35 and B40), and inter‐tribal (DR4, B40 and B35) heterogeneity of these antigens. 408 Kaingang and 141 Guarani haplotypes were defined by segregation analysis. Of the commonest 10 Guarani and 9 Kaingang haplotypes, only one is shared by both tribes. Significant, positive linkage disequilibrium values for HLA‐A,B; HLA‐A,C; HLA‐B,DR and most HLA‐B,C antigen pairs were also different for the two populations. Genetic distance estimates between these two and another seven South‐American Indian populations, and relative to the major human races (negroids, caucasoids, and mongoloids) reveal a comparatively high degree of divergence between the Kaingang and the Guarani, which is uncommon for Amerindian populations li
ISSN:0001-2815
DOI:10.1111/j.1399-0039.1993.tb02011.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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3. |
HLA class II DNA typing in two Brazilian populations |
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Tissue Antigens,
Volume 41,
Issue 5,
1993,
Page 238-242
M. Elisa Moraes,
Marcelo Fernandez‐Vińa,
I. Salatiel,
Shwuyu Tsai,
J. Roberto Moraes,
Peter Stastny,
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摘要:
Brazil constitutes a melting pot of populations arising from three major groups, including Amerindians, Africans, and Europeans predominantly from Portugal who were later supplemented by migrations from other European countries. Although every possible combination of racial mixture exists in Brazil, we have selected for this study two groups of subjects residing in Rio de Janeiro. A predominant White population, among whom some Amerindian admixture may exist, and a predominantly African population having little admixture from the other races. We have used the polymerase chain reaction (PCR) and hybridization with oligonucleotide probes to perform a complete typing of the HLA class II alleles. We report the allele frequencies for HLA‐DRB1, DQA1, DQB1 and DPB1. We also report on the postulated DR‐DQ haplotypes based on family studies and observations in homozygous B‐cell lines. These results may serve as background for various types of clinical studies in Brazilian popula
ISSN:0001-2815
DOI:10.1111/j.1399-0039.1993.tb02012.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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4. |
Rapid DNA typing for Class II HLA antigens: Subtyping of DRw52‐associated DRB1 alleles |
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Tissue Antigens,
Volume 41,
Issue 5,
1993,
Page 243-248
C. Horne,
P. A. Keown,
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摘要:
Serologic typing for MHC class II antigens is incapable of identifying important subtypes for certain DRB1 alleles and occasionally leads to errors of assignment, particularly with the DR antigens associated with DRw52. To simplify DNA typing of DRw52‐associated DRB1 alleles, we have developed a new rapid method using PCR‐RFLP. The PCR‐RFLP method is based on allele‐specific amplification followed by digestion of PCR‐amplified DNA with restriction enzymes. Group‐specific amplification of the second exon of DR3, DR5, DR6 and DR8 was achieved using a 5’primer specific for the first hypervariable region sequence common to all alleles in this group and generic 3’primers. Human genomic DNA was amplified in a Perkin‐Elmer Thermocycler. The presence of a 265 bp fragment was confirmed by agarose gel electrophoresis. Restriction enzyme digestion using Rsa I followed by polyacrylamide gel electrophoresis gave a pattern unique for some alleles and placed the remainder in subgroups. Digestion of the PCR product with one or two of the following enzymes (Asp 700, Hae II, Mnl I, Mbo II, Ksp I and Hph I) permitted the identification of 21 of the 22 alleles. DRB1*1103 and DRB1*1104 are not distinguished by this method and can be distinguished by SSOP or by using a specific 3’primer. For some heterozygous combinations, additional primers are used to provide full subtyping. This method provides a rapid and less costly alternative to PCR‐SSOP for DRw52 subtyping in the smaller laboratory as only one amplification is required (two primers) for the majority of samples. The patterns produced by restriction digest are easy to interpret and complete subtyping using three additional primers can be accomplished in less than 2 days. This method should significantly improve the selection of unrelated donors for bone marrow or solid organ transplantation and facilitate immunogenetic studies i
ISSN:0001-2815
DOI:10.1111/j.1399-0039.1993.tb02013.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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5. |
Nucleotide sequences of the variable regions of a human monoclonal antibody against HLA‐A1, A23, and A24 |
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Tissue Antigens,
Volume 41,
Issue 5,
1993,
Page 249-254
Takuji Ichihashi,
Kazuaki Kubo,
Tomoki Naoe,
Ryuzo Ohno,
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摘要:
Nucleotide sequences of the heavy and light chain variable (VH and VL) regions of a human monoclonal antibody (4–35–7), which recognized HLA‐A1, A23 and A24, were determined by means of the reverse transcriptase‐polymerase chain reaction. This antibody was generated by Epstein‐Barr virus transformation of lymphocytes obtained from a multiparous donor, followed by fusion with mouse myeloma cells. The VH gene segment belonged to the VHIII gene Family, and used the DXP4 and JH4 gene segments. This VH gene segment had 92.9% homology to the germline gene VH26, and contained 21 nucleotide substitutions. Fourteen of them generated the replacements of amino acids. while 7 failed to generate the replacement. The ratio of replacement to silent mutations in complementarity determining regions (CDRs) was 7.0. The VL gene segment belonged to the VkI gene family, and used Jk4. This VL gene segment showed 96.1% homology to the germline gene HK102, and contained 11 nucleotide substitutions. Seven of them generated the replacement of amino acids, while 4 failed to generate the replacement. The high ratio of replacement to silent mutations in CDRs of the VH gene segment suggested that the multiparity caused the processes of antigenic selection and somatic mutation, and generated this anti‐H
ISSN:0001-2815
DOI:10.1111/j.1399-0039.1993.tb02014.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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6. |
A novel HLA‐DPB1 allele (DPB1*4501) in a Dutch caucasian healthy control |
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Tissue Antigens,
Volume 41,
Issue 5,
1993,
Page 255-258
Niek Vries,
Jules P. P. Meijerink,
Hsnk Tijssen,
Miriam Demas,
Ewald J. B. M. Mensink,
Lao B. A. Van De Putte,
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ISSN:0001-2815
DOI:10.1111/j.1399-0039.1993.tb02015.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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7. |
Identification of a new HLA‐DPB1 allele detected by PCR‐RFLP and its nucleotide sequence determination by direct sequencing after PCR amplification |
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Tissue Antigens,
Volume 41,
Issue 5,
1993,
Page 259-262
Nobuhisa Mizuki,
Shigeaki Ohno,
Kazuhito Sugimura,
Takeshi Seki,
Nobuhiko Mizuki,
Liao Geng,
Misaki Ishioka,
Hidetoshi Inoko,
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ISSN:0001-2815
DOI:10.1111/j.1399-0039.1993.tb02016.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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8. |
A novel HLA‐DQB1 allele: Evidence for gene conversion event promoted by χ‐like sequence at DQB1 locus |
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Tissue Antigens,
Volume 41,
Issue 5,
1993,
Page 263-266
Francesco Cucca,
Francesco Muntoni,
Rosanna Lampis,
Fulvia Frau,
Antonio Cao,
Strfano Virgiliis,
Mauro Congia,
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ISSN:0001-2815
DOI:10.1111/j.1399-0039.1993.tb02017.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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9. |
Analysis of genetic variables in selective human IgG3 deficiency |
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Tissue Antigens,
Volume 41,
Issue 5,
1993,
Page 267-268
Mohammed S. Hassan,
Olle Olerup,
C. I. Edvard Smith,
Lennart Hammarström,
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ISSN:0001-2815
DOI:10.1111/j.1399-0039.1993.tb02018.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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10. |
New DP sequences: Three DPA1 and one DPB1 |
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Tissue Antigens,
Volume 41,
Issue 5,
1993,
Page 269-272
Lisbeth A. Guethlein,
Wilma B. Bias,
Barbara J. Schmeckpeper,
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ISSN:0001-2815
DOI:10.1111/j.1399-0039.1993.tb02019.x
出版商:Blackwell Publishing Ltd
年代:1993
数据来源: WILEY
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