摘要:

Abstract:In the present work the kinetics of class II MHC expression on OX8 + lymphocytes generated by skin allograft and its relationship to the lytic activity were studied. Mononuclear cells from the spleen of LEW (RT11) rats bearing BN (RTln) skin graft for 3, 5 or 7 days were sorted out by sequential immune affinity using columns of Degalan‐V26 beads treated with anti‐rat or anti‐mouse Ig. After depletion of B cells, T cells were precoated with W3/25 MoAb (anti‐CD4 equivalent) and sorted out using an anti‐mouse Ig column. The W3/25‐/0X8 + cells (CD8 equivalent) were then coated with OX4 MoAb (anti‐RT1.B) or murine A.TH anti‐A.TL alloantiserum (anti I‐E, cross‐reacts with RTl.D) and were passed through a new anti‐mouse Ig column in order to obtain the four subpopulations, RTl. B +,. B ‐,. D + and. D ‐. Their specific lytic activity against BN Con A‐stimulated cells increased from the 3rdto the 7thd after the skin graft. The lyhc activity observed on the 3rdand 5thd was associated with all four subpopulations analyzed. In contrast, on the 7″ d, the lytic activity was concentrated in the RTI.B+ subpopulation. These results, associated with the increase in the number of OX8 +/RT1.B + cells along with days after graft, suggest that RT1.B expression is not essential but is associated with the effectiveness of the cytotoxic activity. It is also possible that RT1.B expression is a marker of cyt

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01807.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:By use of the heterohybridoma technique we have produced a human monoclonal antibody (NDS40) which detects a split of HLA‐DRwl 3 (DRwl3b) which is in linkage with HLA‐DQwl. In addition, the antibody reacts with cells positive for HLA‐DRw8 and DRwl 1, but does not react with the commonly found split of DRw1 3 (DRw1 3a) which is associated with DQwl. NDS40 is cytotoxic and is of the lambda IgM

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01808.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:Familial Mediterranean Fever (FMF) is an autosomal recessive disease manifested by recurrent short episodes of fever associated with polyzerositis. Although the biochemical defect is unknown, there are several immunologic abnormalities which have been described in this disease. To examine critically whether there is linkage between FMF and the immunogenetic region (major histocompatibility complex‐MHC) on chromosome 6, including the HLA, BF, and GLOl loci, blood samples from members of 13 nuclear Armenian families were tested for these genetic markers. These 13 families included 28 affected and 42 unaffected family members. Linkage could be excluded at 7.5% recombination with the HLA ABC and D loci. Linkage could be excluded at 2.5% recombination for GLOl. Linkage could not be excluded with BF individually, but can be rejected based on the haplotype data. No association was found with either BF, GLO1, and HLA DQ alleles. HLA DR4 was found more often in affected cases than in controls; however, after adjusting for the number of antigens tested this was not significant. Our results would appear to exclude the immunogenetic region on chromosome 6 from linkage with FMF in the Armenian populatio

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01809.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:Two consensus sequences, called × and Y boxes, capable of binding nuclear proteins and regulating expression in B cells have been defined within the immediate upstream region of major histocompatibility complex (MHC) class II promoters. Unlike other class II promoters, the HLA‐DR a (DRA) promoter also contains one element identical to the “octamer” motif of immunoglobulin variable region promoters that is responsible for B cell‐specific transcription. This “octamer” in the context of DRA appears capable of binding both the ubiquitous (OTF‐I) and lymphoid‐specific (OTF‐2) “octamer” binding proteins, but at least one other distinct “octamer” complex was found. In order to characterize the function of cis‐acting elements, we have developed anin vitrosystem in which a DRA promoter construct is transcribed more efficiently in extracts from B cells than in extracts from class II‐negative HeLa cells. 5′ deletion constructs which lacked the Y box, but retained the “octamer” the Y box reduced transcription by 95%. Using supercoiled, but not linear templates, we observed differences in transcription efficiencies from templates lacking or disrupting the × consensus element that reflect effects of random replacement of × box sequences in transient expression assays. Demonstration of the complete dependence on the Y box in this system suggests that, despite its demonstrated importance in the DRA promoter, the DRA “octamer” does not utilize OTF‐2 in a manner anal

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01810.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:The existing estimates of the recombination fraction between DR and DP are quite variable and often based on anecdotal observations. We have estimated the DR/DP crossover frequency on the basis of families typed for HLA markers and GLD. The frequency of DR/GLO crossing over was 8.7% (23/264 informative meioses), maternal recombinations being about twice as frequent as paternal ones. Of 17 DR/GLO recombinant families typed for DPwl‐6, DP was informative in 11 (13 recombinations) but only one of these gave rise to a DR/DP crossover. According to these data the DR/DP recombination fraction is below I%, in contrast to some earlier published materials. HLA‐DR/DP haplotypic associations on 127 informative Caucasoid haplotypes have been evaluated. In agreement with previous studies, DR3 was positively associated with DPwl and, in addition, DR7 was found to be positively associated with DP‐blank (not DPwl‐6). The rare DPw6 allele is possibly associated with the DR4, Dw14 allele. The DR‐DP haplotype profiles suggest other associations which might become significant if larger materials are tested. The frequency of DP alleles in a random material (N = 201) was found to be in accordance with most of the previously published frequences on European Cauca‐soids with DPw4 as the predominating frequency (gene frequency 40%) and a blank frequ

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01811.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:One‐hundred‐and‐nineteen normal Black Zimbabweans were typed for HLA antigens. We found a close similarity between the HLA antigen frequencies in Zimbabweans and Black South Africans (Xhosa), another Bantu‐speaking group. The only significant differences noted were for HLA‐Aw36, ‐Bw53, ‐Cw6, ‐DR4 and ‐DQw3 (p<0.0l). The most common HLA haplotypes in significant positive linkage disequilibrium in the two groups were different. The differences that were noted may be due to gene flow from the San to the Black South Africans, since it is thought that the Zimbabweans had little if any con

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01812.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:HLA‐A, B, C, DR and DQ typing was done on 26 black Zimbabweans with rheumatoid arthritis and the respective antigen frequencies were compared with those in a group of 119 normal individuals from the same ethnic background. Only the DR4 antigen was significantly increased in frequency in the patients, confirming the association with this disease seen in other Black African population

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01813.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01814.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:In a previous work we showed that the two functionally different specificities DPw4 and DPa could only be differentiated by RFLP analysis using two mutually exclusive fragments (respectively, Bgl II 5.29 kb for DPw4 and 7.24 kb for DPa). The DP Workshop synthetic analysis localized these fragments in the DPA2 pseudogene region. Our results demonstrate, however, that they are located between the A1 and B1 genes; the Bgl II restriction site responsible for the 5.29 kb fragment was localized between the first and second exon of the DPBl gene and inside the 7.24 kb fragment. A single mutation point inside this restriction site is responsible for the absence of the 5.29 kb fragment, changing the specificity attributed by RFLP typing.

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01815.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01816.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY