ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1995.tb02470.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Abstract:The novel HLA‐Cw*0704 allele, previously detected as the PCR‐SSP variant Cw7/8v, has been cloned and sequenced from the homozygous typing cell KR03/4 after amplification by anchored PCR. The nucleotide sequence of Cw*0704 is closely related to those of other Cw*07 alleles, but carries specific changes in exon 3 consistent with its serological behavior ‐ a short Cw7 cross‐reactive with antibodies directed against HLA‐Cw8. Some of the substitutions of Cw*0704 have not been previously described for HLA‐C but are found in HLA‐B alleles and in published C sequences of non‐human primates. The new allele carries a novel polymorphism in its 5′ untranslated region (5′ut) that could be shared by all Cw*07 alleles. By PCR‐SSP, Cw*0704 is a relatively common allele in English Caucasoids at a frequency of 4.6%. It is most often observed on HLA‐B44 haplotypes previously described as HLA‐C “blank”, although linkage disequilibria with other HLA‐

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1995.tb02471.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Abstract:The HLA‐B35 serotype represents a group of antigens detectable by IEF, cytotoxic T cells, and by sequencing analysis. Four isotypes and eight alleles have been thus far reported. We have determined the relative frequencies of these B35 subtypes in a group of 203 unrelated people. Dot blot hybridization of PCR amplified products was performed using 23 sequence‐specific oligo probes designed based on the EMBL HLA class I sequence database. The amplification was achieved by a pair of group‐specific primers, producing ˜600 bp fragments. By hybridization pattern analysis, we found that four alleles represent over 95% of the B35+ population, with relative frequency of 48.2% for B*3501, 23.7% for B*3502, 15.2% for B*3503, and 8.0% for B*3508. We also identified 3 individuals with B*3504 and one with B*3505, and seven samples with new patterns. B*3501 and B*3503 exactly correlated with the most common isotype B35.3, B*3502 and B*3504 with B35.2, B*3508 may be the B35.1 IEF isotype. The B*3505 was identified from an individual with B35 IEF variant form. Our study shows that the B35 antigen has a wide distribution of alleles, and that many more B35‐related alleles may yet to be u

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1995.tb02472.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Abstract:CD50, the intercellular adhesion molecule‐3 (ICAM‐3), is expressed almost exclusively on hematopoietic cells. T lymphocytes display a bimodal distribution on CD50 expression levels. It was observed that CD45RO+cells expressed higher levels of CD50 than CD45RA+T lymphocytes. A similar situation was observed when CD4 and CD8 sub‐populations were analyzed, with CD8+cells expressing higher levels of CD50 than CD4+ cells. When adult T lymphocytes were analyzed by three‐color flow cytometry in CD8+CD45RA+cells both CD50lowand CD50highexpressing cells were detected, in accordance with several memory markers on T lymphocytes, whereas only cells with a low level of CD50 were observed in CD4+CD45RA+. The different level of CD50 expression was confirmed by analyzing purified CD45RA+and CD45RO+T cells. Moreover, after the comparison of CD50 expression level in thymocytes, cord blood and adult T lymphocytes, a progressive increase was observed. When T cells were sorted by their intensity of CD50 expression, only CD50highcells proliferated in response to tetanus toxoid. Therefore, the phenotypic and functional analysis of adult and cord blood T lymphocytes as well as thymocytes indicates that CD50 expression increases during the maturation process of T lymphocytes: from the lowest CD50 levels present on CD1+thymocytes, to the highest levels of CD50 on human memory T cells. In addition, we have observed that after CD50 cross‐linking on human T lymphocytes, a transient increase in intracellular calcium concentration ([Ca2+]i) is produced. When CD45RA+and CD45RO+T cells were analyzed, in spite of the level of CD50 expression, the stimulation through CD50 induced a similar level of Ca2+mobilization in both subpopulations, contrasting with the higher rise in [Ca2+]iinduced by CD3 stimulation on CD45RA+versus CD45RO+. These data suggest that the signal transduction pathways activated after CD50 cross‐linking are, at least partially, independent of those involved after CD3

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1995.tb02473.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Abstract:In a previous study we demonstrated that the treatment of the highly tumorigenic cell line, P815, with 8‐methoxypsoralen and long‐wave‐length ultraviolet radiation resulted in the production of several immunogenic clones (tum—‐). Mice inoculated with the tum‐cells survived much longer than mice inoculated with the original tumorigenic cells (tum+). It was suggested that the increased survival of mice treated with the tum‐clones arose as a result of an increased antigenicity derived from the phototreatment. In this report we show that the tum‐cells have a greater density of class I MHC molecules on their surface (50–157% compared to P815). Class I MHC density on the cell surface is required to elicit targeted cytotoxic responses. These results can be considered in terms of human class I MHC assays which show that many human tumor cells have a reduced expression of class I MHC. Because other DNA damaging agents have also been shown to enhance class I expression, it is suggested that in addition to the cytotoxic effects of these agents, other pleiotropic effects must be considered. Photochemotherapy may phenotypically alter cells so that the enhanced expression of class I MHC molecules on the surface of phototreated cells may be associated with the clinical responses observed in cutaneous T cell

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1995.tb02474.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1995.tb02475.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1995.tb02476.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1995.tb02477.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1995.tb02478.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1995.tb02479.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY