ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02237.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:Gametic associations of a three‐allele polymorphism of the HSP70‐I promoter region were analyzed in a random North Italian population, in 69 HLA homozygous cell lines and in 29 families in Boston, all typed for HLA class I, class 11 and complement alleles. Significant phenotypic associations were detected in the random population between HSP70‐1 alleles and several HLA markers carried by extended haplotypes. The inclusion of HSP70‐1 alleles in extended haplotypes, suggested by population analysis, was confirmed in genotyped cells, including 10W HLA homozygous cell lines and families, selected for the presence of the whole set of alleles reported for conserved extended haplotypes. Every tested extended haplotype was exclusively associated with a given HSP70‐1 allele, except those carrying DR1. HSP70‐I C was included in both [HLA‐B8. SCOI, DR3] and [HLA‐B18, FlC30, DR3]extended haplotypes, accounting for the previously observed strong association with DR3. In addition the same allele was found on the [HLA‐B13, SC31, DR71, on the [HLA‐B62, SB42, DR4] and on the [HLA‐B60, SC02, DR131 extended haplotypes. The HSP70‐1 A allele was carried by all DR4+extended haplotypes except the one above cited. HSP70‐1 B correlated with DR10, DQB1*0501 and BF*E Thus the HSP70‐1 promoter alleles provide new precisely located mar

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02238.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:The functional significance of plasma HLA class I antigens is unclear. They are bought to have an immunomodulatory role and be tolerogenic in transplant settings including the materno‐fetal semi‐allo‐graft. There is, however, no available data on the concentrations of soluble HLA class I antigens in fetuses or newborns. We therefore determined plasma HLA class I antigen levels in 93 neonates born at different gestational ages and compared them to those in 66 healthy adults. The mean plasma HLA concentration in cord blood obtained from these neonates (0.30±0.15 μg/ml, mean±SD) was significantly lower (p<0.0001) than in the adults (0.77 ± 0.44 μg/ml). No correlation between the plasma HLA levels and the gestational ages of the neonates was detected. Characterizing the plasma HLA class I antigens by immunoprecipitation and immunoblotting, four different molecular weight forms, 44, 39, 36 and 34 kDa, were recognized. Their distribution in neonates was not different from that in adults. Since the circulating leukocytes are a probable source of plasma HLA class I antigens, we measured the surface HLA expression on leukocytes in 4 neonates and 4 adults by immunofluorescent flow cytometry. The fluorescence intensities on neonatal granylocytes and lymphocytes were 50% of those on corresponding adult cells. This finding suggests that the reduced HLA expression by neonatal leukocytes may be partially responsible for the lower concentration of HLA class I antigens in neona

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02239.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:Mexican American patients (n = 35) with insulin‐dependent diabetes mellitus (IDDM) and control subjects (n = 39) were HLA‐DQA and DQB typed by the polymerase chain reaction technique combined with allele‐specific oligonucleotide probes. Either DQB1*0302 or DQB1*0201 was present among 91% (32135) of the patients compared to 67% (26/39) of controls. Either DQA1*0501 or DQA1*0301 was present in all patients (100% or 35/35) compared to 29/39 (74%) (OR 12.06 Pc<0.05) of controls. All four of these genes, in cis or trans, were present in 15/35 (43%) of the patients compared to 3/39 (WI) of controls (OR 9.0; Pc<0.01). The presence of one or more non‐susceptibility alleles showed a dose‐related decrease in relative risk. Presence of aspartic acid (Asp) at position 57 of the DQ β chain did not confer protection and non‐Asp homozygozity did not confer susceptibility to IDDM in this ethnic group. In conclusion, susceptibility to IDDM in Mexican Americans is associated with particular DQA and DQB combinations, illustrates dose‐dependent parameters and contradicts the critical resi

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02240.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:Class I regulatory complex (CRC) located in the 5′‐upstream region of MHC class I. gene contains transcriptional enhancing sequences, called Enh A. This Enh A region contains tandem‐arranged κB‐ like sites, one of which has a well‐conserved perfect palindromic sequence. The second κB‐like site, juxtaposed to the perfect palindrome, contains an imperfect palindromic sequence. In B‐cell nuclear extracts, we have identified at least four sequence‐specific protein complexes; three shared the repeated κB enhancer as their binding motifs. The perfect palindromic sequence facilitates the binding of a complex termed BI, while the imperfect palindrome provides the binding sites for two other complexes, BII and BIII. The BII and BIII complexes exhibited binding crossreactivity with other κB‐related motifs and recognized both the perfect and imperfect palindromic sequences, whereas the BI complex was specific for the perfect palindromic sequence which is unique to the class I promoters. A DNA segment outside the repeated KB enhancers probably binds the fourth complex, BIV. These complexes, except for the perfect palindrome‐binding complex, differ from those described for the murine class I promoter. The binding characteristics of these factors suggest that the mechanism controlling the class I transcripti

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02241.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:Sixty‐four patients with Takayasu's arteritis and 156 healthy individuals in the Japanese population were examined for HLA‐B specificity at the DNA level by DNA typing using polymerase chain reaction (PCR)/sequence‐specific oligonucleotide probe (SSOP) analysis and by subsequent sequencing analysis. The frequency of epitope combination group‐B52 (EC‐B52) corresponding precisely to HLA‐B52 specificity and that of EC‐B39.2 which is a newly‐identified subtype of HLA‐B39 specificity were increased in the patient group. These two disease‐associated HLA‐B alleles share an epitope composed of63Glu and67Ser. Because two HLA‐B alleles, HLA‐B51 and B39.1, which are similar but different at the epitope from HLA‐B52 and B39.2, respectively, are not associated with Takayasu arteritis,63Glu and67Ser are supposed to be i

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02242.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:We describe sequence‐specific primer (SSP) combinations for use in a one‐step polymerase chain reaction (PCR) typing system to determine HLA‐A locus subtypes of A9 (A23, A24), A10 (A25, A26, A43), A28 (A*6801, A*6802, A*6901) and A19 (A*2901, A*2902, A*3001, A*3002, A31, A32, A33) from genomic DNA. SSP's were designed on the basis of the amplification refractory mutation system (ARMS) in which a mismatch at the 3′ residue inhibits non‐specific amplification. The SSP combinations described extend our low‐resolution typing system, to provide a high‐definition typing of th

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02243.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:A monoclonal antibody 137BL7 raised against purified DR1 protein was shown to bind specifically to 5/5 DRl, 181 18 DR2 cells and 0/23 non‐DR1,2 cellsby cell‐EIA. Further analysis by FACS using HLA‐transfectants revealed that 137BL7 also bound specifically to the DRB5 transfectants in addition to the expected DRl transfectants. However, it did not bind to the DR2 (DR15) transfectants, showing that crossreactivity with DR2 cells lies with the DRB5 (DR51) rather than the DRBl gene product. A comparison of the HLA‐DR amino acid sequences of DRl and DR51 antigens revealed a common glutamic acid residue at position 96, which may form the putative binding epitope of t

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02244.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:Occurrence of clinical neurologic multiple sclerosis (CNMS) among resident Faroese began between 1943 and 1973 and comprised three epidemics. The occupation by British forces for 5 years during World War II was interpreted to have been of major importance for the occurrence of these epidemics and led us to believe that CNMS is the rare, late result of a single, widespread, systemic and specific infectious disease which we have labelled the primary MS affection (PMSA). In this study we describe the occurrence of genetic markers of the HLA system in 16 Faroese MS patients, 25 of their siblings, 30 unrelated healthy neighbors and spouses to MS patients, 18 healthy controls from areas where no MS cases have been detected, and 80 unrelated normal Faroese. These studies show no significant deviations of HLA class I antigens, whereas the class II antigens do deviate: 50% of the Faroese MS patients carry the HLA‐DR2 (DQI lDRB 15) antigen, compared to a frequency of 15–20% among the control groups. Also the group of siblings of MS patients showed an increased frequency of DR4 (72%) compared to normal frequency among MS patients and other normal controls (43–47%). However, if DR 15‐positive individuals were excluded, this difference was further reduced. If PMSA was widespread within this group, DR4 or some closely associated genetic marker may confer protection against PMSA developing into CNMS. The occurrence of CNMS in these epidemics seems therefore associated to HLA class II‐linked genetic factors similar to those found in studies of other Caucasians with MS. This observation seems important in understanding the pathogenesis of thi

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02245.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY