摘要:

An examination was made of blastogenic response to phytohaemagglutinin (PHA) in HLA‐B8 and/or DR3 positive subjects and B8/DR3 negative individuals. Both B8 and DR3 antigens were associated with a depression of the response at all three doses of PHA used. The possession of both these antigens did not lead to a further depression of the respons

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb00482.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

C57BL6 mice which do not express I‐E gene products were immunised with EBV transformed human B cell lines to generate MoAbs. Three hybridoma supernatants which initially reacted with the immunising donor cell but not a T cell line lacking Class II antigens were further investigated.I‐D SDS‐PAGE patterns of molecules precipitated by the three supernatants from a cell membrane lysate were characteristic of HLA‐Class II alpha and beta chains. Two‐dimensional analysis established the specificity of the supernatants as HLA‐DR specific. This was confirmed by the reaction patterns with Class II mutant deletant cell lines. In both ELISA and cytotoxicity one reacted with all lymphoblastoid cell lines tested, one reacted with all except two that were DR7 homozygous and the third reacted strongly only with cells that were DR3. All three antibodies were cytotoxic to both peripheral blood lymphocytes and EBV transformed B cell lines. The DR3 specific MoAb (IgG2a) was suitable as a typing reagent.The DR3 reactive MoAb specificially inhibited stimulation by a Dw3 HTC and the other two MoAbs inhibited all HTCs tested. These findings are consistent with the view that certain determinants responsible for the Dw specificities are carried on the D

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb00483.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

HLA‐A, B, C and DR antigens were studied in 88 patients from Martinique. A modest but significant reduction of B14 and Bw42 antigens was noted as well as an increase in B35 (p<0.05) and DR1 antigens (corrected p<0.05), two antigens known to be in linkage disequilibrium. These data, which corroborate a previous study for B35, suggest that the unusual antistreptococcal response mounted by rheumatic fever patients is under HLA contro

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb00484.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

HLA types, serum autoantibodies and serum globulin levels were surveyed in 46 patients with HBsAg‐negative chronic active hepatitis. Patients with chronic active hepatitis with viral risk factors were less likely than those without viral risk factors to be HLA type B8 (10% vs 44%) or to have autoantibodies (antinuclear and/or anti‐smooth muscle antibodies) (38% vs 84%). Thirty patients (10 with and 20 without viral risk factors) were treated with glucocorticosteroids. Of the 11 patients who were HLA‐B8, 100% responded to treatment. Of the 20 patients who were ANA positive, 80% responded. The data suggest that the presence of HLA‐B8 may be a useful predictor of response to anti‐inflammatory treatment and may define a genetic subset of HBsAg‐negative chronic active hepatitis that will benefit from glucocorticoster

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb00485.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

HLA‐D/DR alleles as defined by cellular and serological typing can also be identified by biochemical methods. The Southern blot technique provides an additional typing facility which can be applied to DNA obtained from any source of nucleated cells. The polymorphism revealed by Southern blot analyses, the so‐called restriction fragment length polymorphism (RFLP), depends upon the restriction enzyme and cDNA probes used. To identify HLA‐DR specificities a protocol was developed based on the use of the results of southern blot analyses with several restriction enzymes and cDNA probes within a panel of HLA‐D/DR homozygous cells representing the DR1 to DRw8 alleles.First, hybridizations with the 3’untranslated sequence of the DRβ cDNA probe, after digestion of the DNA with PvuII (PvuII‐DRβ3′) allows the selective identification of DR1, DR2 and DRw8; DR3, DR5 and DRw6 are found as one group as well as DR4 and DR7 as another. Second, TaqI‐DQα hybridization allows the splitting of DRw6‐Dw18, DRw6‐Dw19 and DRw6‐Dw9 from the DR3, DR5 and DRw6 group. The other alleles DR3, DR4, DR5, DRw6‐Dw16 and DR7 are revealed by dehybridization and rehybridization of the blot with a DRβ cDNA probe. This protocol was used to test whether in a panel of 30 randomly chosen individuals the HLA‐DR typing could be performed. The results were highly concordant to the serotyping. Furthermore by adding the Pst‐DRβ and TaqI‐DQα RFLPs, most of the MLC defined Dw specificities could also be identified. An overview of the specific fragments described here has been summarized in matrices which can be used as references for

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb00486.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Human B‐cell lines secreting antibodies which react preferentially with the HLA Class II antigen DR5 have been produced. Supernatants from these cell lines reacted with lymphocytes from all DR5 positive donors and a minority of DR6 positive donors but were negative on lymphocytes of other phenotypes. The cytotoxic activities of the supernatants apparently depend on IgM antibody. These results demonstrate the potential of B‐cell lines for thein vitroproduction of antibodies to HLA‐DR ant

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb00487.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

A monoclonal antibody, 1B7, which was established by immunizing C57BL/10 mice with splenocytes from B10.BR, was investigated by serological, immunochemical and functional analyses in mouse, rat, guinea pig and human systems. 1B7 recognized a polymorphic determinant on class II antigens in the mouse system. In the rat system, however, this antibody appeared to recognize a monomorphic epitope shared by all RT‐1 haplotypes. 1B7 showed no reactivity in the human and guinea pig strains teste

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb00488.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

A total of 317 unrelated Danish patients with insulin‐dependent diabetes mellitus (IDDM) have been HLA‐DR typed and the antigen and phenotype frequencies compared with those in 1177 unrelated Danish controls. The strong positive associations with DR3 and 4 and the strong negative one with DR2 were confirmed, and the remaining antigens showed a hierarchy from weakly positive to strongly negative associations: DRw9, w8, 1, 5, w6, 7. The study population included various special groups of patients selected in order to study heterogeneity: very early (40 years) survivors without complications. When comparing these groups, the following minor differences were seen: the DR3,4 phenotype is significantly (p = .02) more frequent in IDDM with onset before age 20 (35%) than in other cases (24%), and in familial IDDM (48%) than in other cases (28%); the frequency of the DR4 antigen was significantly (p = .008) more frequent in long‐term survivors (86%) than in other patients (69%), while it was significantly (p = .02) less frequent in IDDM nephropathy (63%) than in long‐term survivors. However, apart from the age‐at‐onset heterogeneity, which was suspected a priori, these differences may be due to chance, and the main conclusion of this study is that the HLA‐DR associations in IDDM are indeed extraordinarily homogeneous irrespective of the clinical characteristics at onset and course

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb00489.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Thirty‐six unrelated Danish patients with pauciarticular Juvenile Chronic Arthritis (PJCA) and 120 controls were typed for HLA‐DPw1‐w6 and the local specificity CDPHEI with bulk‐expanded Primed Lymphocyte Typing (PLT) cells. The frequency of HLA‐DPw2 was 52.8% in PJCA patients and 16.7% in controls (relative risk, RR = 4.5; P<0.001). The antigens HLA‐Dw5 and/or Dw8 were present in 50% of the patients and in 21.3% of the controls (RR = 4.2; p<10‐3). DPw2 was not associated (in linkage disequilibrium) with Dw5/w8 in patients or in controls, and the DP and D associations with PJAC were independent of each other. However, the combined presence of DPw2 and Dw5 and/or Dw8 gave a significantly higher risk of PJCA than each antigen alone indicating interaction of DP and DR gene products. PJCA is the first disease definitely found to be associated with

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb00490.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Drug induced toxic epidermal necrolysis and Stevens‐Johnson syndrome are weakly associated with HLA‐B12. Among patients reacting to a given drug we observed stronger links, especially for HLA‐A29; B12; DR7 and sulphona

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1986.tb00491.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY