ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01917.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:Sixty‐four patients with Takayasu arteritis and 317 healthy individuals in the Japanese population were examined for HLA‐A, ‐B and ‐C alleles by serological typing and for HLA‐DRY DQ and DP alleles by DNA typing using PCR/SSOP analysis. The frequencies of HLA‐Bw52, DRB1*1502, DRB5*0102, DQA1*0103. DQB1*0601 and DPB 1*0901 alleles were significantly increased and the frequencies of HLA‐Bw54, DRB1*0405, DRB4*0101, DQA1*0301, DQB1*0401 alleles were significantly decreased. Strong linkage disequilibria among the increased alleles and among the decreased alleles were evident in the Japanese population. Therefore, the combination or haplotype of HLA‐Bw52‐DRB1 *1502‐DRB5*0102‐DQA 1*0103‐DQB 1*0601‐DPA 1*02‐DPB 1*0901 may confer susceptibility to Takayasu arteritis while another combination or haplotype of HLA‐Bw54‐DRB1*0405‐DRB4*0101‐DQA1*0301‐DQB1 *0401 may confer resistance to the disease. Because this is the first evidence for the association between an HLA‐DP allele and Takayasu arteritis, we examined the nucleotide sequences of the DPB 1*0901 allele from a patient and her healthy relatives and found no difference. The disease is ther

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01918.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:Czechs (CZ) and Gypsies (GY) have lived in the same country for several centuries but seem to have different migratory origins. We have studied 99 CZ and compared them with 34 GY from an isolated group living in Czechoslovakia. DRBI, DRB3, DRB4, DRB5, DQAI, DQB1 and DPB3 alleles were determined by PCR followed by oligonucleotide hybridization. DRB 1*0701 was the most frequent DR allele in CZ (af=36.3%). In GY, after DRB1*0701, DRB1*1404 was the second most frequent allele (af=29.4%), while in CZ this allele was not found. Both groups differed also in the distribution of DR4 subtypes. In CZ, DRB 1*0401 was the most frequent subtype (50.0%) of DR4. In GY, DRB 1*0408 was the predominant type (44.4%). A new DQB1 pattern was found in GY, which we have called DQB1*3.4. This allele was similar to DQB 1*0301 in codon 26, but in codon 57 it resembled DQB 1*0302. All cells having this new allele were also positive for DRB1*0408. Four‐locus associations in CZ showed haplotypes similar to those previously found in other Caucasoid populations. In GY we observed more unusual haplotypes, some previously seen in the Orient, possibly reflecting GY ancestr

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01919.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:The HLA‐B locus is extremely polymorphic. We have sequenced a region, CL, telomeric of HLA‐B that also shows a high degree of allelic variation which we have shown previously by RFLP analysis. The polymorphism can be accounted for by sequence variation in duplicated, reiterated sequence elements called geometric elements. Comparison of the CL1 and CL2 sequences from the 57.1, 8.1, 18.2 and 7.1 ancestral haplotypes revealed that the lengths of the elements vary, both between the duplicated loci within a haplotype and between haplotypes, apparently because certain sequences are inserted or deleted. It is possible, using the polymerase chain reaction, to amplify these elements in genomic DNA from ancestral haplotypes for which sequence data of the CL region are not available and to obtain gel patterns which are characteristic of different ancestral haplotypes. The most striking feature of the data is the fact that the majority of the CL patterns are haplospecific;i.e.have a particular pattern that is unique for a particular ancestral haplotype and can be used to type these ancestral haplotypes. At least 12 different allelic patterns have been identified within a panel of 29 cell lines representing 16 ancestral haplotypes. For these 16 ancestral haplotypes, all examples of each haplotype have the same CL pattern. The haplotypic nature of the patterns confirms that ancestral haplotypes are conserved chromosomal segments and that coding and non‐coding sequences are identical by descent from a remote anc

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01920.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:Previous studies have associated skin test sensitivity and specific IgE response toAmbrosia artemisiifoliaV(Amb aV) with HLA‐DR2, and toAmbrosia trifidaV(Amb tV) with HLA‐DRw52 haplotypes in atopic individuals. Using HLA class II typing by restriction fragment length polymorphism (RFLP) analysis with DRB, DQB and DQA DNA probes to define the HLA‐D alleles, we have demonstrated the association of the DQw6 in 16 out of 16 (100%)Amb aV‐responsive individuals, compared to 3 out of 18 (17%) ragweed‐sensitive butAmb aV‐nonresponsive individuals (p = 5.7 × 10‐6, RR>75). We suggest that the DQw6 association withAmb aV sensitivity may be a reflection of an association with the DQA*0102 allele. This suggests an association of a particular HLA class II allele with an immune response to a well characterized antigen (Amb aV). The HLA‐DRw52 haplotypes in theAmb tV‐sensitive individuals are not of one particular subtype. The HLA‐DRw52 association withAmb tV sensitivity may reside in homologous DRB1 alleles linked on HLA‐DR

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01921.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:It is known from historical records that the population of Cartagena (Colombia) is a mixture of caucasoids (Spaniards) and negroids (Africans) that is named mulattos. The present study was carried out to determine the distribution of HLA antigens in this population. A sample of 370 individuals was HLA serologically typed; 91 of them were typed for Complement alleles and 47 were DNA typed using PCR amplification and oligonucleotide probes for the HLA‐DQAI locus. The analysis of the allele and haplotype frequencies obtained, together with the genetic distances calculated with other populations, showed that most of the HLA genes of mulattos are from negroids and Caucasoids. The Asian and Amerindian contribution is small, consistent with the historical and socio‐anthropological documentation. For example, A23, Aw34, Aw36 and Bw45, as well as DQA1*0101, DQA1*0301 and BfF have frequencies similar to that observed in negroids. In contrast, many significant differences were observed when comparing the antigen and allele frequencies of mulattos with that of Asians and Amerindians. Our results add evidence to the notion that the arrival of Spaniards in America 500 years ago established several racial mixtures. The HLA characterization of one of these, mulattos, will allow a better application of the HLA technology in the fields of basic research, organ transplantation and forensic medicine among this populat

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01922.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01923.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01924.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01925.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb01926.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY