摘要:

The HLA class I and II phenotypes of the three popuation groups in the Cape Province of South Africa were determined. The HLA‐A, B, and C antigens were tested in 1027 South African Negroes (Xhosa), 3716 Cape Coloureds and 1059 South African Caucasoids. This is the first study which has also included the class II antigens in the Southern African Negroes (Xhosa). The numbers tested for the DR and DQ antigens were smaller, as only typings done after the 8th Histocompatibility Workshop were included. A comparison was made between the frequencies in the Xhosa, the Cape Coloureds and the South African Caucasoids as well as the Nigerians, another group who also belong to the Bantu‐speaking division of African Negroes and who were recently studied. The antigen, gene and haplotype frequencies were estimated in all three groups, and the genetic distances calculated. Striking differences in gene and haplotype frequencies between the various populations were seen. For example, Bw42 had a phenotype frequency of 0.062 in the Cape Coloureds, 0.213 in the Xhosa and 0.004 in the South African Caucasoids. The Xhosa showed marked differences in HLA distribution compared to the other Negro group (Nigerians), which can be attributed to a Khoisan admixture, e.g. HLA‐DR4 had a phenotype frequency of 0.134 in the Xhosa and only 0.010 in the Nigerians. The haplotype B8, DR3, seen in association with many autoimmune diseases, had a significant delta value in all three popula

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb02072.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

An LD defined split of HLA‐DR2, different from HLA‐Dw2, Dw12 and DB9, is observed in the Austrian population. The narrow HLA‐D specifity, defined by a new Austrian Homozygous Typing Cell (HTC), a primed lymphocyte (PLT) cell line and a PLT clone, is tentatively termed HLA‐D “WH”. Investigation of three informative families shows that HLA‐D “WH” segregated together with HLA‐DR2. In one family, even an individual heterozygous for HLA‐DR2/Dw2 and HLA‐DR2/D “WH” can be identiñed. A panel study including 90 non‐related Austrians shows that HLA‐D “WH” occuring in 6.7% of the whole panel, that is in 20% of the HLA‐DR2 positive panel members, is completely included in HLA‐DR2. HLA‐D “WH” might be comparable to other LD specificities re

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb02073.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Blood samples from 240 unrelated healthy Tamil‐speaking South Indian Hindus residing in Madras (capital city of Tamil Nadu, India) were screened for HLA‐A and ‐B antigen profiles. Antigen, gene and haplotype frequencies were calculated and compared with the literature. Tamil Hindus lack A31, A32, Aw33, B16, B21 and Bw41. However, except for minor differences (low occurrence of Awl9 antigen), the South Indians show similarity to North Indian and other Indian groups. The data confirm once more that the haplotype A1‐B17 is characteristic of

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb02074.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

A panel of 43 homozygous and 36 heterozygous highly selected cells, representing the most common DR‐specificities, were investigated with the DNA hybridization technique. By using a single restriction enzyme, TaqI, and two probes, DRβ and DQα, it was possible to construct assignment criteria giving a reasonable definition of DR1, 3, 4, 5, 7 + w9, w8, w10 and w11. The criteria sometimes require that certain bands must not be present. Therefore, in certain genotypic combinations, the presence or absence of the particular specificity on one haplotype cannot be decided. This is a problem only for DR2 and DRw6, which for this reason cannot be assigned in about 1/3 to 1/4 of the cases. The association between RFLP assignment and serological assignment is not absolute, the correlation coefficients ranking from 0.62 to 1.0. In the case of false negative RFLP assignment, this may be due to genetic heterogeneity, as in the case of a DR2 individual who proved to be Dw12 and not Dw2 associated. It is often stated that interpretation of the RFLP pattern is particularly difficult in random or heterozygous individuals compared to proven homozygotes. This is not the case in the present study, where in fact correlation coefficients between RFLP and serologically determined DR specificities were higher in the heterozygotes (range 0.79–

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb02075.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Monoclonal antibodies to monomorphic determinants of the MHC class II region products, HLA‐DR, DP and DQ were used to investigate their expression on cells of the normal and coeliac (both treated and untreated) small bowel. HLA‐DR antigens showed a characteristic distribution in the normal small bowel: epithelial cells in the apical portions of the villi stained heavily, and this staining decreased in intensity towards the villous base. The crypt epithelial cells were clearly unstained. Stellate cells within the lamina propria were also HLA‐DR positive. HLA‐DP antigens showed a similar distribution, although the intensity of staining was less than that seen with HLA‐DR. HLA‐DQ antigens were absent from epithelial cells and were confined solely to cells within the lamina propria. In untreated coeliac disease, the intensities of both HLA‐DR and HLA‐DP were increased, and in addition a more extensive distribution of these antigens was observed. In addition to occurring on the surface epithelial cells, DR and DP antigens were now present on crypt epithelial cells. Despite this change in expression of DR and DP antigens, the distribution of HLA‐DQ was essentially unaltered from that of the normal small bowel. The findings in treated coeliac disease were intermediate between those in the normal and untreated coeliac small bowel. The differential expression of class II antigens by normal and diseased small bowel epithelium which has been demonstrated may have implications for interactions of these cells with cells of

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb02076.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Southern blotting with a DPβ cDNA probe of MspI digested DNA from 83 healthy unrelated individuals revealed a 1.8 kb fragment present in all four individuals (and no others) possessing the newly determined DP specificity, CDP‐H

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb02077.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY