摘要:

The purpose of this study was the RFLP characterization of the DRw10 specificity. Twenty‐two DRw10 cells were tested: the DNAs were digested by seven restriction enzymes and hybridized with DRβ, DQβ and DUα probes. Hybridizaton with DRβ revealed a pattern particular to the DRw10 specificity. with a specific Taql 12.5Kb fragment. Hybridization with both DQ‐specific probes showed that DRw10 is always associated with a special DOw1 subtype: DQw5. Furthermore. at DR and DQ levels, the 22 DRw10 cells behaved homoge

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01646.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Thematchablityof a patient awaiting kidney transplantation is his propensity for being ‘well‐matched’. We present two methods for estimating matchability. It is commonly supposed that phenotype frequency is a good predictor of matchability. We show a correlation of only 0.3 between matchability and phenotype frequency using the definition of ‘well‐matched’ used by the UK Transplant Service. We also show that ABO type and HLA homozygocity can affect m

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01647.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Total of 356 women with various types of pregnancy disorders as well as their husbands were classified in four groups regarding the type of the disorder as follows: 1. Recurrent spontaneous abortions (RSA) of unknown etiology (N = 105) and RSA ‐ primary aborters only (N = 84); 2. Blighted ovum (N = 80); 3. Rh immunization in pregnancy (N = 90); 4. ABO immunization in pregnancy (N = 47). Two groups of couples were used as controls: 1. Couples randomly taken from forensic medicine cases of paternity evaluation (N = 104); 2. Couples having two or more children with HLA immunization in pregnancy (N = 78). The couples from all groups were typed for red blood group antigens of ABO, Rhesus, MNSs, Kell, Duffy, Lewis, Kidd and P systems and also for HLA antigens. Significantly higher frequency of antigen HLA‐A9 was found in women with RSA (corr. p = 0.0003) and in women with pregnancy disorders caused by Rh immunization (corr. p = 0.0136). In couples with RSA the degree of HLA compatibility was significant (p = 0.0048) and the reactivity of spouses in MLR was significantly decreased (p = 0.0001). Significantly, more low responders in MLR were also found among the women with RSA as compared to the controls (p = 0.0217). Two possible pathologic mechanisms may explain the association between HLA antigens and RSA: 1. immunological defects which are linked to HLA‐D/DR region causing malfunction of immunosuppressive mechanisms during pregnancy; 2. endocrinological defect which is linked to HLA region as 21‐OH hydroxylase deficien

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01648.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Haplotypes including HLA, Bf and C4 loci were analyzed in a material comprising 55 families with diabetic children. One hundred and ten haplotypes found in IDDM patients were compared with 101 haplotypes present only in healthy family members. Two complotypes,BfSC4A3B3andSC4A0B1. were significantly more common (P<0.05) in the diabetic haplotypes, and these were in most cases found in haplotypic combinations withHLA‐B15,Dw4,DR4andHLA‐B8.Dw3,DR3genes, respectively. TheB8/DR3haplotype was better conserved, as 72% included theBfSC4A0B1complotype as compared with only 35% of theB15/DR4haplotypes with “high risk”C4A3B3complement alleles (p<0.05).DR3was found in 26% of the diabetic haplotypes andDR4in 43%.DR4associated with theDw4in 69% of cases and with Dw14in 26% of the diabetic haplotypes. Our results confirm that the two phenotypes found earlier to be associated with IDDM in Northern Finland, e.g. “B15, BfS, C4A3B3, Dw4, DR4” and “B8, BfS, C4A0B1, Dw3, DR3” are inherite

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01649.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

To study the stimulatory capacity of HLA‐DRβIII in the mixed lymphocyte culture (MLC) assay, an MLC matrix between 13 Dw 18 homozygous typing cells (HTCs) was analysed. Six of these HTCs were positive for the HLA‐DRβIII allele LB‐Ql as defined by T cell clones. Seven HTCs were positive for LB‐Q4. The MLC responses between Dw18 HTCs, matched for LB‐Q, were significantly lower than the responses between the mismatched combinations. Considering the fact that HLA‐DP can induce proliferation in MLC, we then analysed the DP matched and mismatched combinations separately. The influence of HLA‐DRβIII mismatches was in our experiments comparable to the influence of mismatches for HLA‐DP. Surprisingly, 15 out of 22 DRβIII and DP matched combinations still showed pos

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01650.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

A possible genetic contribution to the high incidence of gastric carcinoma in the Cape Coloured population of the Western Cape region of South Africa was investigated. The HLA‐A, B, C, DR and DQ antigens were determined with a microcytotoxicity assay, and their frequencies compared in 124 individuals with gastric carcinoma and 4 560 controls. No significant difference was found, thus supporting the view that a genetic component has a minor, if any, role in gastric carcinogenesi

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01651.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

In 52 conceptuses with known genomic origin, the HLA types expressed on whole villi and/or stromal cell cultures were compared with the HLA types of the patients. No preference for either HLA compatibility or incompatibility was found for androgenetic and paternally derived triploid conceptuses as a whole. Heterozygous, androgenetic, conceptuses, however, showed a trend towards preferential incompatibility, which may be of importance for their apparent greater risk for tropho‐blastic tumour. HLA antibodies were found in 19.2% of the women, i.e. in the range reported for molar as well as non‐molar pregnancies. Non‐HLA antibodies reacting with lymphocytes of the spouse and/or cells from the conceptus were obs

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01652.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

In 52 conceptuses with known genomic origin, HLA determination was performed on whole villi and/or stromal cell cultures. In 26 androgenetic, diploid conceptuses, one or two paternalHLA‐A, Bhaplotypes were found; twenty‐four triploid conceptuses with two paternal and one maternal chromosome sets showed either one maternal and one paternal haplotype or one/two paternal HLA‐A, B haplotypes. Consequently, androgenesis could not be demonstrated by lack of maternal antigens alone. Concomitant expression ofthreeHLA haplotypes was not seen. HLA‐A2 was passed on to the conceptus, both in the genetic subgroups and in the entire series, more frequently than e

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01653.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Erythema multiforme is an acute eruption of the skin and mucous membranes of various aetiologies. Forty‐one unrelated patients were HLA typed for 53 specificities of the HLA‐A, B, C, DR and DQ series. Frequencies of Aw33 and DRw53 were significantly increased: Aw33. 17.0% in patients vs 2.8% in controls (corrected p = 0.01, relative risk = 7.2); DRw.53. 70.7% in patients vs 30.5% in controls (corrected p = 0.0005, relative risk = 5

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01654.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY