摘要:

We have utilized H—2 specific antisera from congenic resistant donor recipient combinations to localize H—2 antigens in tissue sections using the indirect immunofluorescent technique. H—2 specific immunofluorescence is observed in spleen, kidney, and liver sections: however, especially with liver sections, there are cases in which the expected specific stain is weak or absent. In addition to microscopic evaluation by eye, we have employed a microscope attached photometer; recordings from this instrument differentiate positive from control stained spleen sec

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00826.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

An increased frequency of the S allele of Properdin factor B (BfS) was found amongst 162 patients with multiple sclerosis (MS) compared with 470 normal controls. This increase was shown to be due to a strong linkage disequilibrium (LD) between BfS and HLA—Dw2 in 77 patients typed for both systems (Δ= 3.84%,P= .0002). The same LD was demonstrated amongst 100 normal controls (Δ= 2.24%,P= .0049) and 31 patients with idiopathic demyelination of the peripheral nervous system (IDPN). A total of 70 haplotypes with HLA‐Dw2 were encountered (40 MS, seven IDPN and 23 normal controls) and all contained BfS. In the MS patient group, a much weaker association was noted between BfS and HLA—B7 suggesting either that the Bf locus is much closer to the HLA—D than the HLA—B locus or (and) that HLA‐D and Bf products selectively interact (perhaps on the surface of B lymphocytes) with evolutionary advantage or disadvantage resulting from certain allelic combinations. Strong associations between BfS1and HLA—Bw21 (P= .0000) and BfF1and HLA—B18 (P= .0001), both previously reported, were confirmed in the current study. No increase in the frequency of a glyoxalase (GLO) allele was found amongst the MS patients and no LD was encountered between HLA—Dw2 and a GLO allele. The possibility that the HLA—Dw2, BfS disequilibrium has resulted from a selective advantage conferred on the general community but at the expense of increasing susceptibility to MS

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00827.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Seventy‐nine Caucasians with acute myelogenous leukemia (AML) were genotyped to determine whether AML, the induction of remission or patient survival were associated with particular HLA phenotypes or haplotypes. HLA—B17 and B27 were increased in AML patients over 40 years of age. Combined analysis of four independent studies indicates that HLA‐B17 is significantly but weakly associated with AML, relative risk = 1.48 (.01

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00828.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

There is uncertainty about the role of blood transfusion of potential kidney graft recipients. In this study baboons were transfused under conditions mimicking the clinical situation and then challenged with kidney allografts. Eleven baboons were transfused 10 times with blood from different donors. Lymphocytotoxic antibodies induced against the separated B and T cells of a panel of 12 normal baboons were as follows: B−T−30%; B+T−3%; B−T+1% and B+T+66%. Kidney transplantations were done from donors against which the recipients had negativé B−T−cross‐matches. Median allograft survival time (MST) of 12 days in this group, which was immuno‐suppressed with azathioprine and prednisolone, was not prolonged when compared with non‐transfused, immunosuppressed animals (MST 12 days). A second group of 14 animals was screened for cytotoxins after each transfusion against a panel of five to six normal baboons. The antibody profile of 80 combinations tested after three transfusions was as follows: B−T−65%; B+T−16%, B−T+0% and B+T+19%. Transplantation was with donors against which the transfused recipient had formed B+T−antibodies. Kidney allograft survival was again not prolonged (MST 13 days) but the mean serum creatinine level 8 days after transplantation was significantly lower than that

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00829.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

We examined 188 men who had undergone vasectomy up to 6 years previously and, for comparative purposes, 100 men who were about to undergo the operation. Blood specimens were available from a total of 283 men.Sperm antibody assays using immunofluorescence, microagglutination, and microimmobilization confirmed that the prevalance of several types of antibody is higher after vasectomy. Immobilizing and agglutinating antibodies appeared to be the most important. Trends in antibody prevalence according to the time after the operation were analyzed. Screening against lymphocytes and lymphoid lines showed that the anti‐sperm activity of sera was not related to anti‐HLA or anti‐Ia activity. Associations were examined between different types of sperm antibody, and between these antibodies and autoantibodies to other antigens.When antibody prevalence was studied in relation to HLA types, the HLA antigen A28 was found to be strongly associated with production of head agglutinating antibody (and immobilizing and immunofluorescent equatorial antibodies) after vasectomy. This is one of the first clear‐cut examples of antibody production associated with the HLA

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00830.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

In response to multiple injections of liver extract from Balb/c mice, DBA/1 mice produce antibodies against a component of the liver preparation which is not F‐antigen, but which is able to precipitate in gel diffusion tests. This antigen is also found in extracts of Balb/c lung, but not in a variety of other organs tested. Liver extracts from all of the inbred strains studied contain the antigen, except extracts from DBA/1 mice. Furthermore, this antigen was not found in the liver or lungs of other vertebrates tested. The antigen has been designated antigen

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00831.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

A random population of 108 Black North‐Americans, 79 of whom had also been typed for HLA—D in Mixed Leukocyte Culture (MLC), were serologically tested for DRw with Seventh International Histocompatibility Workshop trays. Clusters of B cell alloantisera were identified which defined several DRw antigens. Although the clusters for DRw1, DRw2, DRw3 and DRw7 showed similarities with the clusters derived during the Seventh International Histocompatibility Workshop, distinct differences were observed which should be taken into account for the assignment of the DRw antigens in Blacks. DRw6 was defined by a cluster of sera which also reacted with DRw1 and DRw2 cells. The tails of these sera were used to assign DRw6, but this was only possible with DRw1 and DRw2 negative cells. It was not possible to identify serum clusters specific for DRw4 orWIA8.The associations between DRw and the corresponding Dw specificities were reasonably good for DRw1, DRw2 and DRw7, but weaker for DRw3 and DRw5. The cumulative gene frequencies for DRw1, DRw2, DRw3, DRw5, DRw6 and DRw7 was 0.86. The frequencies of these six DRw antigens fitted into a Hardy‐Weinberg distribution, suggesting that DRw in Blacks is controlled by a single gene with multiple al

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00832.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

Sixty‐one patients in the Dundee area suffering from psoriasis were typed for HLA—A and HLA—B antigens. On the basis of the typing results, the patients were divided into three groups, and studied with respect to sex, age of onset and familial incidence of the disease. The frequency of HLA—A1 appeared to be increased and HLA—B7 decreased but HLA—B13 and HLA—B17 were highly significantly increased (P<10−6andP<10−10respectively) in the psoriatic group compared to 204 controls. Of particular interest was a highly significant association of HLA—A1 with HLA—B17 in psoriatic patients. Family studies showed HLA—B17 to be a useful genetic marker for psoriasis in the families of B17 positive patients. Considerations of age of onset, familial incidence and typing data suggest that there is heterogeneity of genetic susceptibility to psoriasis and that one probable mechanism is the dominant inheritance of a “disease allele” in linkage disequilibrium with th

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00833.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

HLA typing was performed on 267 Welsh coalworkers with pneumoconiosis (96 cases of simple pneumoconiosis, 115 cases of progressive massive fibrosis and 56 cases of Caplan's Syndrome) and 134 coalworkers with no abnormality. The presence or absence of rheumatoid factor was also determined. The results fail to confirm a previously reported increase in HLA—A1 and B18 in coalworkers with no pneumoconiosis. When correction was made for the number of antigens typed (i) HLA—Bw21 was significantly increased from 1.1% in the total group with pneumoconiosis to 8.2% in coalworkers with no abnormality (Pcorrected<0.032); (ii) HLA—Bw45 was increased in Caplan's Syndrome (10.7%) and Caplan's Syndrome patients with rheumatoid factor (16.1%) when compared to a non‐occupationally exposed control group (0.8%) (Pcorrected = 0.019 and 0.0064 respectively). These results were not significant when comparisons were made with the coalworker group with no abnormality. The apparent higher frequency of Bw45 in Welsh coalworkers is di

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00834.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY

摘要:

The incidence of Thromboangiitis Obliterans in brothers and the high prevalence in some ethnic groups have led us to investigate the histocompatibility HLA—A, B and DR antigens of 46 Buerger's disease patients. The main result indicates a marked decreased frequency of the B12 antigen: 2.2% vs 28% in controls. Our study suggests that Buerger's disease is, on the basis of HLA antigens, a distinct immunogenetic entity and not a particular form of arteriosclerosis. Moreover, this disorder may represent a clue to the existence of resistance genes linked to HL

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1979.tb00835.x

出版商:Blackwell Publishing Ltd    

年代:1979

数据来源: WILEY