摘要:

The genomic DNA of 25 unrelated individuals with HLA haplotype A30 Cw5 B18 B/F1 DR3 DRw52 DQw2 was digested with the restiction enzyme Taq I and hybridized with cDNA probes for C4 and 21·OH genes. Eighteen individuals and a non‐Sardinian reference cell (DUCAf) were homozygous for the entire extended haplotype, six individuals were heterozygous for the HLA‐A locus and homozygous for the rest of the same haplotype. All of the 18 homozygous individuals, four of the six heterozygous subjects and the DUCAF cell revealed the absence of two fragments at 3.2 and 2.4 kb corresponding to the 21·OH A gene, and the absence of fragments corresponding to the C4 B “long” or “short” gene. The contemporary absence of the 21·OH A and C4 B genes seems to be a constant characteristic of this extended haplotype in the Sardinian population and suggests that this and other extended haplotypes bearing C4 A or C4 B null alleles could be ancestral haplotypes which never duplicated

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1989.tb01720.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Vasectomized men with sperm auto‐antibodies (S.A.A.) and high frequency of HLA‐A20 antigen (Ag) were observed by Law et al. (1979); Hancock et al. (1983) observed the prevalence of HLA‐A28 in infertile men with S.A.A. HLA‐A, B and DR Ag were determined in a population of 80 Caucasian infertile men with (n = 22) or without (n = 58) agglutinating and/or cytotoxic S.A.A. in serum (S) and/or seminal plasma (SP), and the association between HLA‐determinants and infertility with S.A.A. was investigated. The frequence of HLA‐A28 Ag was found higher in the group with S.A.A. than without (18.2% vs 0.0%) but not statistically significant (X2= 8.87; pc<0.15). The same result was obtained with HLA‐B13 Ag (X2= 8.87; pc<0.32). Lastly, there was no prevalence of HLA‐DR Ag in men with S.A.A. In conclusion, no association was found between HLA‐A, B and DR Ag and infertility with S.A.A. Nevertheless, two HLA‐Ag were more frequently observed in t

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1989.tb01721.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

During the 10thInternational Histocompatibility Workshop (10thWS), 181 HLA MoAbs were studied using lymphocytotoxicity micro‐technique (LCT) and/or enzyme immuno‐assay (EIA), and their capacity to serve as typing reagents was evaluated. 129 MoAbs were tested by both techniques. Results obtained with 92 class I and 86 class II polymorphic MoAbs (10thWS) were compared to published data concerning 180 class I and 176 class II polymorphic MoAbs, listed in an HLA‐MoAbs Register maintained in our laboratory.The following conclusions can be proposed:1/ HLA‐A, B typing by LCT with MoAbs is possible for about 14 specificities. Some specificities are clearly recognized (HLA‐A3, B8, B13, Bw4, Bw6), others are recognized as cross‐reacting groups (B7+27+w22+40), others are not currently recognized by any MoAb with restricted specifity (B5, B15). Several MoAbs confirmed the existence of shared epitopes between products from a single locus (A2‐A28, A25‐A32), or from A and B loci (A2‐B17, Bw4‐A9‐A32). A single HLA‐Cw MoAb has been described.2/ HLA class II typing by LCT with MoAbs is more difficult than class I typing. DR2, DR3, DR4, DR5 and DR7 as well as DRw52 and DRw53 are well defined; other DR specificities are poorly or not at all defined. Particular associations (DR1+DR4, DR3+DRw6, all DR except DR7) are recognized by several MoAbs. All DQw specificities are well recognized, including new specificities defined only by MoAbs: WA (DQw4), TA10 (DQw7), 2B3 (DQw6+w8+w9). Only two HLA‐DP MoAbs have been described.3/ Satisfactory results, similar to those of LCT, were obtained with EIA using lymphoid cell lines as targets.4/ Human MoAbs (12 in the Register) are satisfactory typing reagents. They could represent in the future a significant contribution

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1989.tb01722.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Six mouse and 13 rat monoclonal antibodies (mAb) recognizing HLA‐DR, DQ and DP antigens were used for the detection of cell surface class II MHC antigens of equine lymphocytes. The monoclonal antibodies were tested against peripheral blood lymphocytes (PBL) from a panel of thoroughbred horses, using two‐color fluorescence flow cytometry. Seven of these mAbs reacted with both surface immunoglobulin positive (sIg+) and surface immunoglobulin negative (sIg‐) lymphocytes. sIg+cells stained consistently brighter than sIg−cells. The fluorescence pattern did not vary from donor to donor for each of the mAbs tested, except for SFR1‐MI.2, which reacted with a variable intensity with cells from 47 of 53 horses tested. Immunoprecipitation with mAb SFR1‐MI.2 and analysis by two‐dimensional electrophoresis demonstrated the presence of light and heavy chains equivalent to HLA class II α and β chains. Antibody N297 (DQ specific), previously shown to react with an epitope expressed on human B cells but not on mitogen‐induced T cells, reacted only with sIg+cells in 42 of 53 horses tested. The lack of staining of horse sIg−cells with N297 may be due to a low or lack of expression of this determinant on these cells or to a weak cross‐reactivity of this antibody

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1989.tb01723.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

AITP, a disease with well‐defined autoimmune character, has been previously studied for a possible HLA association. However, no such association with antigens of the A, B, C or DR loci was found. As this does not exclude a possible association with HLA‐DP antigens, we have investigated 36 patients with AITP for DP w1, w2, w3, w4 and w5 by primed lymphocyte typing using locally derived as well as the original DP reagents as used in the 9th International Histocompatibility Workshop. Comparison of antigen frequencies with those of 254 healthy controls revealed an increased frequency of DPw5 in the patient group which, however, was not statistically significant. Other discrepancies were not obser

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1989.tb01724.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

HLA supratypes as well as serum IgG, IgA and IgM levels were determined in 44 children and adolescents with severe IgA deficiency (serum IgA<5 rog/dl) and in first degree relatives. Frequencies of the HLA alleles B14, DR1, DQW1, C4A2 and C4B2 were significantly higher in the IgA‐deficjent patients than in the controls. The most recurrent haplorype among patients was B14, DR1 (p<10−4) preferentially associated with A33, A28 or A blank. The supratype B14, BfS, C4A2, C4B2, DR1, DQW1 was present in a 14‐fold higher frequency than in the controls, and strongly suggests the presence of a gene in the HLA region involved in the deficiency of IgA. The fact that this supratype was not always associated with IgA deficiency in the parents and that not all IgA‐deficient subjects had this supratype is discussed. Severe IgA deficiency was found in four mothers (two of the four mother‐child pairs shared the B14, DR1 haplorype); three other relatives (one father and two brothers) had partial IgA deficiency and did not have the B14, DR1, DQW1

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1989.tb01725.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Eighty‐three south Indian patients with psoriasis vulgaris were studied for HLA antigen frequencies and compared with 77 controls studied simultaneously. HLA Bw57, a split of B17 was found elevated in the patients. The two sexes differed in their age‐at‐onset curves: females had a preponderance to early onset of the disease, while the males had late onset. Among these patients, major group 3, a Western Brachycephal Armenoid group, revealed the highest risk for B17&Bw57 but not major group 2, a Mediterranea

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1989.tb01726.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The frequencies of HLA‐DP alleles in 50 acute lymphocytic, 43 acute non‐lymphocytic, 50 chronic myelogenous and 51 chronic lymphocytic leukaemia patients were compared with 254 controls using primed lymphocyte typing. In CLL and ANLL there were significantly decreased frequencies of DPwl. Decreased DPwl and DPw3 was observed in ALL, but after correction for the number of comparisons made this was no longer significant. However, in ALL, even after correction, there were significantly increased frequencies of DPw2 and DPw5, whereas in ANLL and CLL the only significant increases were of DP‐blank, and in CML there were no positive or negative associations at all. These results suggest an influence of DP alleles in disease susceptibility and resistance in three of the four major types of leuk

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1989.tb01727.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

We have HLA typed 46 unrelated IgA deficient blood donors in our region. A weak association to DR3 (RR = 2.07) was observed. However, owing to the heterogeneous nature of this group, we found DR7 may be associated with those who developed class‐specific anti‐IgA antibodies (RR = 2.94), whereas DR1 may be associated with those who did not (RR = 2.

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1989.tb01728.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Thirty‐one patients with endometriosis were typed for HLA‐A, B and DR antigens. No association was found between the disease and any DR specificity, which may be taken as evidence against an autoimmune aetiol

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1989.tb01729.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY