ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb01175.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The monoclonal antibodies B1.23.2 and B9.12 were developed to characterize human class I major histocompatibility gene products. They detect two different epitopes and define on a given lymphoblastoid B cell line two subsets of β2micro‐globulin‐complexed HLA class I molecules. One subset reacts with B9.12 and B1.23.2 and the other one expresses only the B9.12 epitope. Binding assays were performed on C3H mouse L cells which had been transformed with various single HLA‐class I genes and the two detected molecular subsets were shown to be encoded by different genetic loci.Unlike B1.23.2, B9.12 detects all the β2m‐complexed molecules expressed in human B cell lines and recognizes an epitope different from the one defined by W6/32. In contrast to the vast majority of the other reported anti‐class I monoclonal antibodies (including B9.12), B1.23.2 recognizes an epitope expressed on both β2m‐associated and ‐free HLA clas

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb01176.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

HLA antigens A and B were determined in a group of 41 patients with congenital hypothyroidism and 36 of their mothers. Twenty‐nine patients had thyroid dysgenesis of whom 23 were functionally athyreotic, four had ectopic and two had hypoplastic thyroid glands. Twelve patients had thyroid dyshormonogenesis, seven of whom had iodide organification defect. A total of 48 antigens were typed in the A and B loci. The results were analyzed in comparison to 388 normal adult subjects from the general population. The only statistically significant difference was an increase in the frequency of HLA‐Bw44(12) (48.8% vs 19.3%,P<0.02). The incidence of HLA‐Bw44(12) was 44.2% in the mothers. There was a decreased frequency of HLA‐B7in the patients which was not sign

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb01177.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The chromosome 6 markers, HLA‐ABC, D, DR, MT, properdin factor Bf, and complement factors 2 (C2) and 4 (C4), were studied in three families, each of which included two HLA identical siblings, one or both of whom were known to be HLA‐B: GLO recombinants. The families were also typed with primed lymphocyte typing (PLT) for HLA‐D/DR region associated DP antigens. None of these studies gave evidence that the recombinations had occurred within the HLA region. Mixed leucocyte culture (MLC) tests within the families showed no detectable stimulation between the HLA identical siblings in two of the families, but a very weak stimulation between the HLA identical siblings (H and G) in the third family (GG). No reactive PLT reagents were generated when cells from the HLA identical siblings of the first two families were primed against each other. In contrast, priming between cells of H and G gave rise to reactive reagents. One of these (GHx), reacted with a determinant which segregated within the GG family as if child G was a paternal recombinant between the HLA‐D, DR, DP, and C4 loci, on the one hand, and on the other hand one or more loci governing other HLA‐D/DR region controlled lymphocyte activating determinants. This reagent was only restimulated by cells from two of 47 unrelated individuals. The other PLT reagent (HGx) did not give a clearcut pattern within the family because it was weakly positive with all family members (most of whom were D/DR2‐positive) except the specific responder; in the panel it reacted with a determinant significantly associated with D/DR/DP2. Other PLT reagents could be generated within the family against lymphocyte activating determinants controlled by genes in the two paternal haplotypes telomeric to the assumed recombinational site. These reagents gave stronger reactions than the HGxand GHxreagents and reacted with two determinants in the unrelated panel strongly associated with D/DR/DP2 and D/DR/DP6, respectively.It seems likely that the GG family represents a third example of a recombination between the HLA‐DR and SB loci. Our findings further support the assumption that the DR determinants may be immunodominant in lymphocy

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb01178.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

A murine allo‐immune A.TH anti‐A.TL (anti‐Iak) serum pool and sera from individual immunized mice were tested on a large panel of human DR typed B cells, obtained from peripheral blood (PBL—B cells), and human lymphoid tumour cells. The anti‐Iakserum pool appeared to be a useful reagent both in cytotoxicity and immunofluorescence for the examination of the presence of class‐2 HLA molecules on tumour cells. Only one discrepancy was observed when the pool was tested in parallel with a xeno‐anti‐human B‐cell serum on the cells of 56 lymphoid tumours. Nine sera from individual mice were tested on a panel of 93 PBL—B cells. Some of these sera reacted significantly more weakly with human B cells positive for the DR5

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb01179.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

This report describes the influence of HLA‐D/‐DR antigen disparity upon the level of cytotoxicity in allogeneic in vitro cultures.Allogeneic cultures, between unrelated HLA‐D/‐DR full house donors, tested in CML gave three different levels of cytotoxicity, termed weak, intermediate and strong cytotoxicity. HLA‐D/‐DR compatibility predictsweakcytotoxicity and two HLA‐B antigen incompatibility predictsstrongcytotoxicity. On the contrary, HLA‐A antigens have no major influence upon the strength of cytotoxicity.Accepting that the MLC/CML reaction is an in vitro parallel to the in vivo transplantation of allogeneic tissue, the observations are in accordance with the results of HLA‐D/‐DR matching for graft survival in human re

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb01180.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

HLA typing was performed in two groups of individuals with low serum IgA concentrations. These consisted of 44 individuals identified from a blood donor clinic and 37 individuals attending an Immunology clinic with disorders associated with IgA deficiency. Both groups showed an increase in the frequency of HLA B14 (P<0.0001), HLA‐A28 (P= 0.0007) and the combinations HLA‐A1, B14 and HLA‐A28, B14. The previously reported increase in HLA‐A1, B8 was not apparent in either group. These data suggest that there is a gene within the human major histocompatibility complex which is relevant in IgA de

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb01181.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Thirty‐two patients with malignant hypertension and terminal uremia and a group of 1263 healthy blood donors were studied regarding the incidence of different HLA antigens. HLA‐Bw35; Cw4 antigens were found to be significantly more frequent in the group of patients with malignant hypertension than in the group of healthy individuals. The frequency of HLA—Bw35 in a group of 60 non‐uremic patients with biopsy‐proven glomerulonephritis and without malignant hypertension was also studied and found equal to the group of healthy blood donors. There was no overrepresentation of IgA nephritis among the HLA—Bw35 positive patients with glomerulonephritis, but advanced vascular lesions were more common among these than among patients with other H

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb01182.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

When a disease is rare in the general population, genotype relative risks can be estimated from case‐control data by adjusting the genotype frequencies in controls to the expected Hardy‐Weinberg proportions. The resulting risk estimates are shown to have a smaller variance than those obtained from the observed genotype frequencies, and test statistics are given for adjusted estimates. The tests are shown to have a larger power for detecting genotype association, and heterogeneity in the genotypic relative risks, than those based on the observed frequencies. An application is given to data on rheumatoid arthritis and HLA‐DR ant

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb01183.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

LIF release by Con A stimulated mononuclear cells was evaluated in 67 randomly selected healthy Sicilians typed for HLA antigens. The results show that B8 and/or DR3 positive subjects release less LIF than negative ones, suggesting that this immunological response might be controlled by HLA‐linked immune response (Ir) gene(s

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb01184.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY