摘要:

Abstract:The molecular reaction patterns of the DRw52‐specific mouse monoclonal antibodies UL‐52 and 7.3.19.1 were investigated. Upon immunoprecipitation and two‐dimensional IEF‐SDS polyacrylamide gel electrophoresis analysis (2D‐PAGE) mAb UL‐52 selectively isolated DRβ1 molecules from DRw52‐positive cell lines, whereas mAb 7.3.19.1 predominantly precipitated DRβ3 molecules. Reduced mAb UL‐52 binding affinity was observed to DRw8‐ and DRwl2‐positive cells, potentially resulting from structural modifications within the antibody binding site. Comparison of mAb UL‐52 reactivity with published DRβ chain amino acid sequences demonstrates that the amino acid residues ‐S‐ in positions 11 and 13 on DRβ1 molecules essentially contribute to the formation of the antibody binding site. mAb 7.3.19.1 reactivity, on the other hand, correlates with the expression of DRβ3 chain amino acid residues K, G and N, in positions 71, 73 and 77, respectively. In contrast to other DRw52 monoclonal antibodies described so far, mAb UL‐52 demonstrates a similar reactivity to DRw52 allosera, suggesting that mAb UL‐52 and DRw52 allosera possibly recognize the same or a sim

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01828.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:The major part of the proliferative response in primary mixed lymphocyte cultures (MLC) is caused byHLA‐DRB1incompatibilities. InDRB1‐matched pairs the proliferation induced byHLA‐DRB3, ‐DQ and ‐DP mismatches may be unmasked. In most previous studies the influence of HLA‐DP incompatibilities in primary MLC has been investigated in homozygous typing cells representing only a few Dw specifities. We were interested in determining the stimulatory capacity of isolated HLA‐DP mismatches, ascertained by RFLP analysis, in primary MLC in HLA‐A, ‐B, ‐DR and ‐DQ compatible, unrelated heterozygous individuals of many different Dw specificities. Thirty‐eight MLCs performed with cells from related pairs and 67 with cells from unrelated pairs were evaluated. All but nine of the MLCs were analyzed in both directions, giving a total of 201 investigated reactions. The relative responses (RR) in the three MLCs performed between DP incompatible, related pairs were all positive (RR ≥ 8%). Eighty of 82 MLCs performed with cells from DP incompatible, unrelated individuals were positive, whereas 37 of 46 MLCs between DP compatible, unrelated pairs were negative (RR<8%) (p<10−10). The magnitude of the RR was influenced by the number of DP mismatches. Thus, the mean RR was approximately twice as high in MLCs in which responder and stimulator cells differed by two DP antigens (mean RR 60.5%) compared with reactions with only one DP mismatch (mean RR 35.4%) (p<10−3). RFLP‐defined HLA‐DP incompatibilities predict a positive primary MLC in HLA‐A, ‐B, ‐DR and ‐DQ matched individuals with a high degree of accuracy (98%). We suggest that in the search for matched unrelated bone marrow donors HLA‐DP typing might preferably precede and in many instances even replace MLC. This may well facilitate the search for compatible unre

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01829.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:The CDw50 differentiation antigen is defined by 101–1D2 and 140–11 monoclonal antibodies (mAb), both produced and characterized in our laboratory. This molecule is broadly expressed on hematopoetic cells but not on other cells. In this report we show that these 2 mAb recognize different epitopes of the same molecule, which are resistant to neuraminidase and proteases. We also demonstrate that the CDw50 antigen is expressed on thymocytes and T lymphocytes as an N‐glycosylated glycoprotein monomer with a relative molecular weight (Mr) of 130000 daltons with intrachain disulfide bonds, and that this molecule is resistant to treatment with phosphatidylinositol (PI) phospholipase C and therefore probably not PI‐anchored to the membrane. CDw50 is a poorly or non‐constitutively phosphorylated molecule that becomes phosphorylated by treatment with phorbol 12‐myristate 13‐acetate (PMA) of peripheral blood mononuclear cells (PBMC). The addition of affinity‐purified CDw50 mAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. The inhibition of alloreactivity is mediated at the level of both responding and s

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01830.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:The major characteristics of the canine CD18 leukocyte antigen family, defined by a murine monoclonal antibody CA1.4E9, are described. The overall molecular organization of this family of leukocyte integrins was similar to the human counterpart, and consisted of a common beta subunit of 95 kD (CD18) and non‐covalently linked alpha subunits of 150 kD (CD11c), 165 kD (CD11b) and 180 kD (Cd11a). Conservation of CD18 epitopes between dog, human, feline, equine and bovine was observed. CD18 expression in lymphoid tissue had a characteristic pattern which was based on differences in the intensity of expression observed in different cell types. The expression of LeuCAMs in canine epidermotropic lymphosarcoma (mycosis fungoides) was also explored since LeuCAMs might be expected to play a major role in the tissue tropism of this tumor. Intense CD18 expression was observed in the malignant T cells which infiltrated the epidermis; these cells differed phenotypically from dermal T cells in that they often lacked Thy‐1 expression. The potential pathogenetic significance of LeuCAM expression in canine mycosis fungoides is discus

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01831.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:A new monoclonal antibody raised against gradient‐purified feline immunodeficiency virus was found to recognize a bimolecular complex, comprising 27–29 kD and 32–35 kD subunits, on feline peripheral blood lymphocytes. Immunoperoxidase staining of feline tissues with this antibody, designated 43.2H2, demonstrated a reactivity pattern similar to that described for MHC II antigens of the dog, horse, and pig, but differed from human and mouse in having staining of T‐cell zones in spleen and lymph nodes. Flow cytometric analysis revealed that 42.3H2 reacted with 88.97%± 16.00% of feline peripheral blood lymphocytes (n = 20). This high level of reactivity was found to be consistent by repeated sampling over a 4‐month period. Two‐color flow cytometric analysis was used to determined the reactivity pattern on lymphocyte subsets: 88.92%± 7.30% of CD4+ lymphocytes were 42.3H2‐positive, while 85.99%± 11.46% of CD8+ cells were positive (n = 11 for both). B lymphocytes had the highest reactivity (99.47%± 0.45; n=9) and also had the highest fluorescence intensity. By gating based on light scatter properties, 95.06%± 7.35% of monocytes were 42.3H2‐reactive (n= 18), while granul

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01832.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:This report describes the organization of the mouse pore‐forming protein (perforin) gene(Pfp), which is highly analogous to that of the corresponding human gene.Pfpcomprises three exons, the first of which consists entirely of 5′ non‐coding sequence, separated by an intron of 1.94 kb from the two polypeptide‐coding exons. The promoter region of the gene shows strong similarity to that in humans, with six stretches of high homology noted within 0.7 kb of the mRNA cap site. However, many of the sequences of the human gene with similarity to previously described promoter/enhancer elements are poorly conserved in the mouse, suggesting that these motifs may be of no functional significance, and that control mechanisms for expression ofPfpmay be highly specific to kille

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01833.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:Genomic typing ofin vitroamplified DNA with sequence‐specific oligonucleotide (SSO) probes was performed for DRB1, DQA1, DQB1, DPA1 and DPB1 alleles in 54 random Norwegian rheumatoid arthritis (RA) patients and 181 healthy controls. DRB1 alleles encoding the serological specificity DR4 were found in 80% of the patients, compared to 34% of the controls (relative risk = 7.9, p<0.0001). All DR4‐positive RA patients carried either DRB1*0401 (Dw4), 0404 (Dw14), or 0405 (Dw15), while no patients were found to carry DRB1*0402 (Dw10) or 0403 (Dw13). The frequency of the DRB1*0101 allele encoding DR1 was not increased, even among DR4‐negative RA patients, and we were unable to detect any sharing of other class II alleles among DR4‐negative patients. No contribution of any DQA1, DQB1, DPA1 or DPB1 alleles to RA susceptibility could be detected. The results suggest that in the Norwegian population RA is primarily associated with a shared sequence at residues 67‐74 of the DRβ1 chain, but only when this sequence is expressed on DR4

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01834.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01835.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY