ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01861.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract:To study the genetic heterogeneity of theC4andCYP21genes in selected primate species we used the technique of restriction fragment length polymorphism (RFLP). Genomic DNA was digested using several restriction endonucleases and filters were hybridized with a 500 bp BamHI/ Kpnl fragment derived from the 5’section of a humanC4‐cDNA and with a 1700 bp BamHI obtained from a humanCYP21gene. Abundant RFLP heterogeneity was observed for theC4genes within a rhesus monkey population but not for the chimpanzee colony analyzed. DuplicatedC4‐CYP21clusters can be traced back in humans, but also in chimpanzees, orang‐utans and rhesus monkeys. Thus, duplication of the basicC4‐CYP21cluster in primates may have happened more than 30 million years ago. Non‐duplicatedC4‐CYP21regions were found for the gorilla and orangutan. Apart from this, shortenedC4AandC4Bgenes were observed in chimpanzees, orangutans and rhesus monkeys, whereas the so‐called long variety of theC4Agene appears to be present in humans and orang‐utans. This ancestral modification, resulting from an insertion of a 6.5 kb intron in theC4Agene, therefore predates at least speciation of human and orang‐utan which is estimated to have taken place more than 1

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01862.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract:We have developed a microcytotoxicity assay allowing sera to be screened for anti‐epithelial cell cytotoxic antibodies. Cells from the epithelial cell line A549 were cultured overnight in Terasaki trays prior to the addition of the sera to be screened. Using this assay, 63 pediatric recipients of 78 renal transplants have been studied retrospectively. Seventeen transplants carried out in 13 patients were found to be associated with the production of antibodies reactive only against epithelial cells (AEC). Eleven of these transplants failed as compared with 19 failures out of 52 transplants not associated with AEC production (Fisher's p = 0.04). We conclude that transplantation in the face of pre‐existing AEC should be approached with caut

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01863.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract:HLA‐DPBl typing was performed using polymerase chain reaction DNA amplification and sequence‐specific oligonucleotide probing (PCR‐SSOP) which permitted identification of 17 distinct DPB alleles using 15 oligonucleotide probes. The accuracy of this approach was confirmed in an initial study of 26 human B‐lymphoblastoid cell lines which demonstrated close agreement between PCR‐SSOP and PLT assigned types. A cohort of 47 adult French Canadians was then studied to provide an estimate of DPB 1 allelic frequencies in an ethnically homogeneous population. DPB1*0401 was the most frequent phenotype (61.5%) and only DPB1*0101, 0301 and 0402 were also present at frequencies greater than 10%. HLA‐DPw4 has been reported to be associated with multiple sclerosis (MS) but our PCR‐SSOP analysis of 52 French Canadian MS patients showed no association with either the DPB 1*0401 or DPB 1*0402 splits of DPw4 or with any othe

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01864.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01865.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract:In the Northern European population, all DR2 haplotypes encoded by DRB1*1501 have previously been found to carry the DQA1*0102 and DQB1*0602 alleles, and DR3 haplotypes have been found to carry the DQA 1*0501 and DQB 1*0201 alleles. Here we report a novel recombinant DR2 haplotype carrying the DRB1*1501, DQA1*0102 and DQB1*0603 alleles as well as a novel recombinant DR3 haplotype carrying the DRB1*0301, DRB3*0101, DQA1*0102 and DQB 1*0602 alleles.

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01866.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01867.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01868.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

The HLA Class I sequences included in this compilation are taken from publications listed in the papers: Nomenclature for factors of the HLA system, 1990 (1) and Nomenclature for factors of the HLA system, 1989 (2). Where discrepancies have arisen between reported sequences, the original authors have been contacted where possible, and necessary amendments to published sequences have been incorporated into this alignment. Future sequencing may identify errors in this list and we would welcome any evidence that helps to maintain the accuracy of this compilation. In the sequence alignments, identity between residues is indicated by a hyphen (‐). An unavailable sequence is indicated by a period (‐). Gaps in the sequence are inserted to maintain the alignment between different alleles showing variation in amino acid num

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01869.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract: The HLA Class II sequences included in this compilation are taken from publications listed in the papers: Nomenclature for factors of the HLA system, 1990 (1) and Nomenclature for factors of the HLA system, 1989 (2). Where discrepancies have arisen between reported sequences, the original authors have been contacted where possible, and necessary amendments to published sequences have been incorporated into this alignment. Future sequencing may identify errors in this list and we would welcome any evidence that helps to maintain the accuracy of this compilation. In the sequence alignments, identity between residues is indicated by a hyphen (‐). An unavailable sequence is indicated by an asterisk (*). Gaps in the sequence are inserted to maintain the alignment between different alleles showing variation in amino acid numbe

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01870.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY