ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb02038.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb02039.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The phenomenon of antigenic modulation was studied in the histiocytic lymphoma line U937. A redistribution of cell surface HLA antigen after incubation of U937 cells with the monomorphic anti‐HLA class I monoclonal antibody W6/32 was demonstrated by immunofluorescence analysis. As assessed by hybridization of RNA obtained from W6/32‐treated U937 cells with a probe corresponding to the α3 domain of HLA Cw3, prolonged W6/32 incubation (24 to 72 hours) induced a decrease in HLA class I transcript abundance. This decrease was about 25% as compared with untreated control cells. These data indicate that W6/32 incubation can induce changes in HLA class I gene expression not only at the antigenic but also at the transcriptional level. Possible implications for the molecular basis of antigenic modulation are discu

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb02040.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

A study on the HLA structure of the Sardinian population was carried out on 551 healthy unrelated,families representing all of the island districts. Altogether 2202 HLA‐A, B, Cw, DR individual haplotypes and 853 different haplotypes were determined. Cavalli‐Sforza and Edwards’genetic distance index for the total of 62 tested alleles showed a modest heterogeneity between one district and another (0.09–0.16). The genetic distance between Sardinians and the rest of the Italian population was 0.23 (0.22–0.26) and progressively increased in comparisons with cauca‐soids (0.26), negroids (0.34) and mongoloids (0.41). Sixty‐three two‐locus haplotypes with a high positive linkage disequilibrium were observed in our family sample. The percentages of two‐locus haplotypes in LD shared with other populations turned out to be 45% with caucasoids, 20% with negroids and 10% with mongoloids. The distribution of the A, B, Cw, DR haplotypes is shown with 673 of them represented only once or twice, and 10 (1.2%) 14–322 times each. Of the latter, 8 are extended haplotypes. 6 of which characterize the Sardinian population. The analysis of our data suggests that the present‐day Sardinian population is the result of a superposition of different populations, mainly consisting of caucasoids on a pre‐caucasoid paleo

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb02041.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Twenty‐four alleles have been defined for HLA‐DPB based on their second exon sequences. This paper describes a novel method, co‐digested amplified fragment length polymorphisms (CAFLP), for assigning these alleles to heterozygous patients, as well as to homozygous cell lines. The method depends on co‐digestion of amplified DNA by restriction endonucleases and separation of the resultant fragments with polyacrylamide gel electrophoresis. Co‐digestion by selected restriction enzymes produces a set of readily discernible fragments that are unique for a given haplotype because the selected restriction sites occur in cis. Consequently, this method provides haplotype information not available from independent digests and allows all known heterozygous genotypes to be identified. Analysis of 103 trios of mother, father, and child, plus 120 normal caucasians, demonstrates the reliability and simplicity of this procedure. This simple typing method results in unambiguous assignment of all current HLA‐DPB genotypes in random samples with a high proportion of heterozygous

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb02042.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The contribution of HLA‐DP genes to celiac disease susceptibility has been investigated in 95 Italian patients, 41 with childhood and 54 with adult disease onset. Polymerase chain reaction amplification, sequence‐specific oligonucleotide probe hybridization and restriction fragment length polymorphism analyses have been carried out. All celiac patients and 56 out of 128 random healthy controls were DQw2‐positive. The frequency of the DPB1*0101 allele was significantly increased (pc= 0.002, relative risk 5.21) in patients with celiac disease (23.2%) compared to the whole panel of controls (5.5%), but not to the 56 controls bearing DQw2 (10.7%). No significant difference in the frequency of DPB1*0101 was found between celiac patients with pediatric (24.4%) or adult (22.2%) onset. The DPB1*0101 allele was associated with both the DR3‐DQw2 and DR7‐DQw2 haplotypes. Moreover, our study has not confirmed the association with DPB1*0402 and DPB1*0301 previously reported in celiac children from southern Italy. The linkage of the DPB1*0101 allele with the DQ locus and the observation that the DP but not the DQ association appears to be ethnically dependent strongly support a secondary role of DP molecules in celiac diseae susce

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb02043.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

We have studied the HLA‐class I and class II antigen distribution in a sample of 75 Spanish Gypsies and 74 Spanish non‐Gypsies by serology, restriction fragment length polymorphism, and protein chain reaction and hybridization with allele‐specific oligonucleotide probes. When both population samples are compared, we find that Gypsies have a statistically significantly higher frequency of A1, A11, B61, Cw6, DQ5 and haplotypes DR16 DQ5 Dw21 and DR14 DQ5 Dw9 DR52b. Frequency of A3, A29, B44, DR4, DQ2, DQ8 and haplotypes DR1 DQ5 and DR7 DQ2 DB17 DR53 are significantly lower in this ethnic group. The analysis of the serological data in the two populations demonstrates that Cw6 can be split into long Cw6 (Cw6.1) and short Cw6 (Cw6.2). Haplo‐type A1‐Cw6‐B61‐DR14‐DQ5 is the most characteristic in Gypsies, with a frequency of 13%. Estimation of the genetic distances shows that Spanish Gypsies are closer to Indian Caucasoid populations than to the Spanish non‐Gypsy population. HLA data support the proposed historical origin of

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb02044.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Production of monoclonal antibodies directed against polymorphic epitopes of HLA class II molecules using whole human cells as immunogen has often proved ineffective, because most of the antibodies produced are directed against non‐MHC human cell surface molecules. One approach to overcome this problem is the use of transfected mouse L cells expressing a single HLA class II allele as immunogen. By immunizing C3H mice with DR103‐transfected L cells, we obtained 3 mAb, OHA TM901, OHA TM902, and OHA TM903, that recognize different polymorphic epitopes of the HLA‐DR molecule. The molecular specificities of the 3 mAb were determined on a large panel of B‐lymphoblastoid cell lines (B‐LCL), peripheral blood cells and HLA class II transfectants from the XIth International Histocompatibility Workshop. Interestingly, the 3 polymorphic mAb detect new HLA‐DR epitopes shared by several specificities: OHA TM901 reacts with DR1 (DR101, DR103), DR9 (DR901) and DR10 (DR1001) molecules; OHA TM902 recognizes the same molecules but also DR8 (DR801, 802, 803); OHA TM903 reacts with all DR types except DR3 (DR301, 302), DR7 (DR701, 702) and DR52. Surprisingly, OHA TM901 reacts with DR9 transfectants and B‐LCL but not with DR9 peripheral blood lymphocytes. Biochemical analyses indicate that the 3 mAb immunoprecipitate HLA‐DR products and react in western blots with DR α/β‐dimer but not with free α‐ or β‐chains. This study shows that transfected L cells are very useful tools for the production and the fine characterization of mAb recognizing polymorphic epitopes

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb02045.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

To examine whether unexplained recurrent spontaneous abortion (URSA), defined as 2 or more consecutive spontaneous abortions, is correlated with a particular DR type in the Japanese population, we determined the HLA‐DR types of 82 primary aborters and 21 secondary aborters by DNA typing utilizing the polymerase chain reaction (PCR) and hybridization with sequence‐specific oligonucleotides (SSOs). The DR gene frequencies of the patient group were compared with those of a normal group at three different levels of DR‐definition (27, 13 and 11 DR types). At none of the three levels of comparison was any particular DR type with a frequency differing significantly between the patient and normal groups detected in Japanese URSA patients. Furthermore, we examined whether URSA was correlated with the degree of compatibility of HLA‐DR antigen within patients and their husbands. Comparison of the DR compatibility between patients and normal couples was made in two different ways, i.e., comparison of the numbers of couples with mismatches and comparison of the average number of mismatches. For either of these two comparisons, we observed no difference in DR compatibility between patients and normal couples. Our results suggest that URSA is not correlated with any particular DR type and that the condition cannot be explained simply by DR compatibility between husband a

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb02046.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1992.tb02047.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY