摘要:

Antibodies purified from sera of patients with Burkitt's lymphoma by absorption onto and elution from cultured lymphoblastoid cells, were labeled with125I and repurified by repeating the absorption‐elution procedure. The doubly purified antibodies bound with a high degree of efficiency to immunoadsorbents prepared by entrapping in polyacrylamide gel normal human serum or exudate fluids from patients with ovarian or breast cancer. Binding was specific, as it could be inhibited by high dilutions of human sera but not by animal sera. Globulin fractions of human sera were strongly inhibitory. Their capacity to inhibit was lost after treatment with rabbit anti‐human IgG serum, indicating that at least part of the inhibition could be ascribed to antibodies. Some inhibitory activity was also found, however, in the “albumin” fractions of sera, suggesting that an antigen was also involved. It appears, therefore, that practically all human sera contain antibodies reacting with an antigen(s) which is present on human cells and is also circulating freely in serum and other body fluids. Sera of patients with Burkitt's lymphoma contained, on the average, higher concentrations of these antibodies, as compared to sera of individuals not suffering from this disease. The antigen is thought to be associated with the cell membrane and has been identified in cells of widely differing

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1976.tb01043.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Sixty‐two patients with Crohn's disease and 51 with ulcerative colitis were HL‐A typed. Phenotype frequencies were calculated and compared with those of a control group of 335 individuals. A higher frequency of HL‐A17 was found among the Crohn's disease patients but this was not significant. However, HL‐A17 has been included in two studies on Crohn's disease and the combined relative risk is 3.24 which significantly deviates from 1 unity.No statistically significant differences in the HL‐A antigen distribution were found among the patients with ulcerativ

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1976.tb01044.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

The cytotoxic activity of an anti‐HL‐A2 antiserum cross‐reactive with W28 appeared in three distinct peaks after the serum was subjected to isoelectric focusing. The pH at which the peaks formed was reproducible using pH 5–8 or pH 3.5–10 gradients. Anti‐HL‐A2 activity occurred in each peak, but the cross‐reactive anti‐W28 cytotoxicity appeared to be largely confined to the second and third peaks. The first peak was exclusively IgG, the second contained IgG and IgM, and the third contained

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1976.tb01045.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

This report describes the purification of TL from papain digests of a tumor line (ASL1) and of an established cell line (L251A). Through the use of gel filtration and ion‐exchange chromato‐graphy, the TL was purified approximately 100‐fold with respect to the original digest. It was noted that the TL isolated from ASL1 had a specific activity 1.4 times higher than that isolated from L251A. The reason for this anomaly is unexplained. However, this work indicates that classical methods of protein chemistry can be used in the purification of these membrane components which are present in only small amounts on the cell su

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1976.tb01046.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Permanent human lymphoid cell lines were established successfully from peripheral blood lymphocytes which had been separated for HL‐A typing and stored in liquid nitrogen for two years. Frozen lymphocytes were chosen from two siblings who were homozygous at the LA and FOUR HL‐A loci. Thawed lymphocytes were transformed with EB virus produced by the marmoset lymphoid line B95–8. No chromosome abnormalities were seen on karyotypes prepared on cells from the established human lymphoid lines using G and Q banding techniques. HL‐A typing showed the expected HL‐A antigens plus a considerable number of additional reactions.Separation of lymphocytes and freezing them for possible future use requires a relatively small investment. This method of preserving cells can be applied to patients with interesting genetic disorders or other biochemical markers to provide cells which can be transformed and propagated ye

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1976.tb01047.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

In vitrosensitized cytotoxic T lymphocytes (CTLs) primarily detect antigens controlled within the major histocompatibility complex (MHC) that are associated with the serologically detected (SD) loci LA and FOUR. Frequently CTLs sentitized to cells from one individual kill target cells of a third party that shares no SD cross‐reactivities by serological criteria with the initial stimulating cells. We have studied the possibility that this cross‐killing is due to the recognition of non‐MHC controlled antigens. Allogeneic tissue from MHC identical siblings is rejected in the absence of immunosuppression, demonstrating the importance of non‐MHC antigens. Nevertheless, CTLs sensitized simultaneously with mitomycin‐C treated cells from an MHC identical sibling and from an unrelated individual were only able to mediate cytotoxicity on the unrelated's target cells. No significant cytotoxicity was observed in any combination on target cells differing only for non‐M

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1976.tb01048.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1976.tb01049.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1976.tb01050.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

The incidence of histocompatibility (HLA) antigens in 100 men with myocardial infarction was compared with the incidence in 500 healthy controls. No significant difference was found, providing no support for either a genetic or an immunological basis for myocardial infarction.

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1976.tb01051.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1976.tb01052.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY