摘要:

Flow cytometric analysis and cellular assays were used to determine constitutive and induced expression of class I HLA antigens and class II antigens encoded by the HLA‐DR, ‐DQ and ‐DP subregions on cultured human arterial endothelial cells (HAEC) derived from transplant donors. Class I but no or minimal quantities of class II HLA antigens were found on HAEC. Prior incubation of HAEC with γ‐IFN increased class I HLA antigen expression and induced class II HLA antigen expression on HAEC. The induced expression of HLA‐DQ was lower than that of HLA‐DR, but both were significantly reduced in comparison to the frequency of these antigens on EBV transformed lymphoblastoid cell lines derived from the same donor. In addition, supernatants from class I and class II alloreactive clones were shown to induce class II antigen expression on HAEC. By PLT analysis, it was shown that these antigens are functionally capable of generating a lymphocyte response. In this regard, HAEC have proved to be a helpful tool in designingin vitrolymphocyte‐endothelia

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01663.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

We have previously reported a significant increase of HLA‐DR4 antigen frequency in giant cell arteritis (GCA). This finding suggested an important role of immunogenetic factors in this syndrome. Recent data suggest that inherited susceptibility to several autoimmune diseases was associated with specific DR4 associated DQ β alleles. DNAs from 27 DR4 positive patients with GCA were digested with Taq I and Barn HI, analysed on 0.7% agarose gel and hybridized with DR β, DQ α and DQ β probes. DR β hybridization produced no variant detectable within DR4. DQ β probe confirmed two clusters among DR4 associated DQW3 alleles: DQW 3.1 (Bam HI 360 Kb) and DQw 3.2 (Taq I 1.9 Kb and Bam HI 11 Kb). Among our 27 DR4 positive patients, 34% were DQW 3.1 and 66% were DQW 3.2. These frequencies are the same as those observed in healthy c

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01664.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Clone F‐7 was generated by limiting dilution of lymphocytes stimulated by allogeneic PBL on MLC. Priming against the A23, Cw6, B45, DR7, DRw53, DQw2 haplotype was performed between two HLA haploidentical first degree relatives. The clone was tested for its ability to proliferate in response to a panel of 38 homozygous B lymphoblastoid cell lines plus three local T cell lines. It showed a pattern of reactivity corresponding to HLA‐A9 specificity (r= 1) and presented a concomitant cytotoxic activity. Phenotypically, this clone consisted entirely of CD4 cells, as determined by indirect immunofluorescence. Its reactivity was completely blocked by anti‐DR (GSP4.1, PL8, L243) and anti‐DP (B7/21, PL15) Mo‐Abs, whereas anti‐DQ (1A3, TU22) and anti‐class I (w6/32, BB7.7) Mo‐Abs and anti‐A9 antibodies did not inhibit its reactivity. These results may suggest that clone F‐7 could recognize a DP specificity sharing common determinants with DR, which occurs in linkage disequi

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01665.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

PBMC were isolated from a multiparous woman with HLA‐B27 specific Abs in her serum. The HLA type of the donor was A2,9;B7. The PBMC were EBV transformed, and four cell lines making cytotoxic Abs to HLA‐B27+cells prepared. Hybridomas were constructed by fusing the EBV lines with the human fusion partner KR4. All four mAbs were of IgM isotype. One mAb (TrBH12) reacted specifically with B27+, B37+and Bw47+lymphoblastoid cell lines and with all B27+PBMC except for a rare variant so far found only in one Norwegian family. Another mAb (Tr3B6) was cytotoxic for all B27+cells tested, including the TrBH12−‐ variant; in addition, it showed weaker cross‐reactions to Bw42, B49 and a cell line with the probable phenotype B7,38. Supernatant from the Tr3B6 hybridoma was tested in lymphocytotoxicity against a panel of 658 individuals, 141 of whom were B27+. With this panel, Tr3B6 showed perfect correlation with HLA‐B27. The two last mAbs (TrCG10 and TrBFl) reacted with all B27+cells tested, but in addition showed quite extensive cros

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01666.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The HLA‐DR antigens DRw8, DRw11, DRw12, DRw13, DRw14 and DRw17 are strongly associated with the supertypic specificity DRw52. This association has been used to assist in assignment of serological specificity. However, difficulties in the identification of these antigens arise since they are serologically crossreactive. This report describes the application of restriction fragment length polymorphism (RFLP) allogenotyping to assist in the positive identification of DRw8. DRw11, DRw12, DRw13, DRw14, DRw17, DRw52a and DRw52b, and in addition describes further subtypes defined by RFLP which correlate with DQ or Dw associations. We also describe a novelTuqI/DRβ RFLP in a Caucasian family which types serologically as DRw

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01667.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Two separate studies have shown a distortion in the sex ratio of first born children from HLA‐DR compatible parents (Ober et al. 1985, Radvany et al. 1987). Kil‐patrick (1987) was unable to confirm this distortion on an independent family data set. The question of the HLA‐DR related sex ratio distortion still remained open. In the data set, “Provinces Françaises” families from 15 French provinces and Quebec were tested for a number of genetic markers including HLA‐DR. In 1304 of these families, the HLA‐DR results and family structure information were sufficient to allow the testing of this hypothesis. There were 2265 male and 2156 female children (overall sex ratio: 1.05); 1307 males and 1237 females were HLA‐DK typed. In this group, the sex ratios are little different from those in the overall set, except for the firstborns which exhibit an apparent increase in the sex ratio. When dividing the sample between children fully HLA‐DR compatible with their mother and those incompatible (i.e. having one antigen not present in the mother), the sex ratios in the two groups are little different whatever the birth order. This analysis has failed to observe any significant distortion in the sex ratio related to feto‐maternal compatibility in agreement with the study of Kilpatrick. We conclude that, if such a distortion exis

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01668.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Investigating HLA‐A, ‐B, ‐C, ‐DR and ‐Dw antigens and MLR, CML, and PLT reactivity in two unrelated persons, it was found that, despite their HLA‐D/DR identity, cytotoxic T lymphocytes (CTL) could be induced in the CML assay. The HLA‐DP antigens proved to provide the proliferative impetus for the gene

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1988.tb01669.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY