摘要:

Abstract:Mixed lymphocyte culture (MLC) assays from 763 patients and prospective unrelated marrow transplant donors identified through the National Marrow Donor Program (NMDP) were analyzed for their overall utility in measuring HLA‐D region Compatibility. The assays were performed at 31 different transplant centers (range, 1 to 197; median, 9 assays per center). A total of 325/763 (42.6%) of the tests were judged to be uninterpretable. either due to lack of sufficient control cells included in the assay (89 tests) or to insufficient reactivity by patient and/or donor cells (236 tests). Among the 438 tests that could be interpreted, HLA‐Dw phenotyping with HLA‐D homozygous cells was performed for a subset of 190. The relative response (RR) values from these 190 tests, however, were not clearly separable into distinct populations; i.e., RR values corresponding to Dw identity versus nonidentity between patient and donor could not be reliably discriminated. The predictive value of a nonreactive MLC for Dw identity was calculated to be 0.91 for RRs of ≤ 20%, while the predictive value of a reactive MLC for Dw nonidentity was 0.35 for RRs of>20%. These results, based on an analysis of data submitted from multiple transplant centers testing patients who had a variety of hematologic disorders, suggest that the MLC assay is a relatively imprecise method for determining HLA‐D region compatibility between patient and prospective unrelated mar

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02190.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:DNA typing of HLA class I1 alleles of the DRB1/3/4 and DQBI loci using sequence‐specific oligonucleotide probes and polymerase chain reaction‐amplified DNA was used for the large‐scale typing of donors for the National Marrow Donor Program unrelated donor registry. The results of quality control analysis for the first 7 months of the project show the typing to be highly accurate, specific, and reliable. The percent of correctly classified HLA oligotypes based on 1652 DRB1 and 1652 DQBI assignments was greater than 99% for DRBI/DRB3/DRM and greater than 98% for DQBI. This level of accuracy is particularly remarkable because the quality control samples could not be distinguished from 9011 donor samples tested at the same time by the laborat

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02191.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:A molecular approach to type a new HLA‐B5 antigen, HLA‐BSNA, characterized by its unusual association with the public determinant BW6, referred to as B*7801, has been designed. Antigens disclosing serological identity with SNA, BXI and Te76 were also investigated. Based upon HLA‐B exon 2 group‐specific PCR, the following procedure was established: 5′ and 3′ primers were designed by targetting the codons 11‐12 (AM) and 81‐83 (LRG), respectively, in exon 2 (α1 domain). The 5′ primer discriminates with HLA‐A, ‐C genes and pseudogenes, while the 3′ primer detects the sequence encoding the BW6 epitope (NLRG) and discriminates it from the BW4 epitope. The combination of this pair of primers specifically amplifies 26 HLA‐B alleles. Oligotyping for B*7801 was performed using a combination of two non‐radioactively labeled SSO probes identifying positions T45 and D74 in exon 2. To resolve ambiguous hybridization patterns, an additional set of probes was used. The specificity of this BW6‐group‐specific amplification procedure was investigated on 150 genomic DNA samples. Among them, we obtained 94 amplified DNA products which were tested with eight SSOs. Beside B*7801, the following B alleles could be defined: B*0801, B*35, B*5401, B*5501‐2, B*5601‐2. B*1501‐7 (including 862. B75 and B72) and B*4601. SNA, BXI and Te76 DNA samples gave similar hybridization pattern providing a clue to the identity of these antigens. This “one PCR and two probes” procedure represents a simple oligotyping strategy which can also be appl

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02192.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:Early B‐lineage cells in mice express a cell surface glycoprotein, recognized by the BP‐I and 6C3 monoclonal antibodies, that has been identified as aminopeptidase A (APA E.C.3.4.11.7). In the present studies we obtained evidence by DNA ”zoo‐blot” analysis that the APA gene is highly conserved. This ectoenzyme catalyzes the removal of N‐terminal Glu‐ and Asp‐residues to convert angiotensin II to angiotensin III. a degradation step important in local regulation of blood pressure in mammals. To gain further insight into the physiology of this molecule, which is shared between immune and vascular systems, we examined the tissue distribution of BP‐l mRNA using a cDNA probe and of the protein antigen using the BP‐I antibody for immunohistology. APA transcripts were present in all tissues examined. Abundant BP‐I IAPA was found in the intestinal brush border of the small intestine, renal glomeruli, proximal renal tubules, pulmonary alveolar walls and vascular endothelium in many organs. Other tissues containing the BP‐I antigen included stromal cells in the thymus cortex, bile canaliculi in liver, gall bladder epithelium, interlobular ducts in pancreas, the ovarian theca interna, basement membrane of the epididymis and the splanchnopleure in placenta. APA enzyme activities have been identified in most of these locations in keeping with identification of the BP‐1/6C3 antigen as APA. The data suggest this ectopeptidase may serve diverse physiologic roles in a

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02193.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:HLA‐DRB1 *04 gene variants were determined in apparently healthy Japanese with PCR‐SSCP and PCR‐RFLP, and HLA‐B‐DRB1 *04 haplotype frequencies were estimated statistically. DRB l *0405, which consisted of 60% of the Japanese DRB1 *04 gene pool, was associated strongly with HLA‐B54. This association was observed only for DRB1 *0405, and not for any other DR4 variants. DRB1 *0403 and DRB1 *0406, which share the same peptide sequence except for amino acid 37. were both associated with B35 and B62. DRB1 *0407 was associated exclusively with B35. DRB1 *0410 was associated with B60 and B61. 860 and B61, on the other hand, were associated only with DRB1 *0410 and DRB1 *0405. B35 was associated with all DR4 microvariants. With this possible exception, our calculation suggests that unique B‐DR associations were present even for DR4 microvariants. The knowledge of HLA‐B and DRB1 *04 associations would be useful in cl

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02194.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:We have examined the Taq I restriction fragment length polymorphism (RFLP) of the MHC class II regions of 96 normal Southern Chinese with respect to the HLA‐DRB. ‐DQA, and ‐DQB genes. The data were compared with the DR and DQ serotyping. RFLP correlates well with the serological typing of the DR types of Chinese. Variants are seen in DR2 and DR12. The variant in DR12 with a 10 kb at the upper band (DR12b) is not found in Caucasoids and is more frequent in Chinese (19.4% of the alleles). With the help of DQA and DQB RFLP the assignment of genotyping of DRB types is facilitated. Some of the linkage disequilibrium patterns among DRB, DQA and DQB are different from Caucasoids. This is particularly obvious in the DR2 and DR5 haplotypes. In the 96 Southern Chinese, the 3 commonest haplotype frequencies are: DR12b. DQα1b, DQβ3b (18.3%); DR9, DQα3. DQβ3α (15.7%); DR4. DQα3. DQβ3a (14.2%). The various DRβ. DQα. DQβ genes can be accurately defined in Chinese by RFLP. Polymerase chain reactions using sequence‐specific primers were performed to confirm the various HLA‐DRB and ‐DQB alleles. The use of RFLP is important in the study of HLA matching and HLA‐disease associat

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02195.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:Although the B70 antigen exhibits allele frequencies of 8‐23% in African and American black populations, it remains poorly defined. Cloning and sequencing of cDNA encoding B70 antigens from six cell lines has identified a group of three closely related alleles: B*1503, B*1509 and B*1510, that form a subgroup of the B15 family. The sequences of these alleles and, in particular, B*1503, are close to that of the HLA‐B consensus consistent with the difficulty in their serological definition. The products of the three alleles correspond to three electrophoretically detected variants of the B70 antigen and some correlation with the B71 and B72 subspecificities of the B70 antigen can be made. A fourth allele, B*7901. previously described by Choo et al. (J. Immunol. 147: 174‐180, 1991) that was not serologically typed as B70, differs by a single nucleotide substitution from B* 1510. The sensitivity of alloantibodies to single differences in peptide binding residues suggest a role for bound peptides in the HLA‐970 alloantigenic specificities. The heavy chains encoded by the four alleles differ at four peptide binding residues of the antigen recognition site, the evolutionary modification of which can be explained in terms of interallelic recombination

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02196.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:A new hybrid haplotype, a DRB1 *0103 allele associated with a DRB5 *0101 allele, was found in a French Caucasoid family and has been described here. When these cells were typed by serology, contrarily to cells with the DR1 + 2s haplotype, they did not seem to be triplets. The reactivity of these cells with the DR2 allosera led to a False serological HLA‐DR typing. RFLP analysis and DNA oligotyping after DR1‐DRB1, DR1‐DRB1 and DRB5 group‐specific amplifications showed that there was no DR2‐DRB1 product in these cells and demonstrated the segregation of a DR103 DR51 haplotype in t

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02197.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract:A member of a newHLA‐Alocus family,HLA‐A *8001, has been characterized from 3 African‐American individuals expressing a uniqueHLA‐Aserologic specificity. TheHLA‐A *8001sequence is most closely related to alleles of theHLA‐A1/3/11family, although it also contains residues characteristic of theHLA‐A2/28, ‐A9, ‐A10, and‐A19families. More importantly, theHLA‐A*8001sequence contains four unique nonsynonymous (amino acid replacing) nucleotide substitutions absent from all other primateAlocus alleles. In addition, five other nucleotide substitutions, four nonsynonymous and one synonymous (silent). observed only in non‐human primateAlocus alleles were found. Neighbor‐joining tree analysis ofHLA‐A *8001supports the notion that theHLA‐A *8001allele is a member of a newHLA‐Alocus family which was derived from the ancestral A3 lineage but diverged early in

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02198.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1993.tb02199.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY