摘要:

The HLA‐B14 antigen exists in two serologically distinct forms termed Bw64 and Bw65. These two B14 subgroups were studied using the ability of F(ab')2 fragments from HLA‐B14 and Bw6 antisera to block the binding of cytotoxic HLA antibodies directed towards the same or an adjacent antigenic site. The findings suggest that the B14 antigen consists of several antigenic determinants some of which are spatially distinct and others which are closely associated. A “common” determinant possessed by both B14 subtypes is proposed which is separate from a Bw64 (or Bw65) site. Additional determinants that B14 shares with B8, B18 and B38/B39 appear to be distinct from the Bw64/Bw65 site, variably associated with the B14 “common” determinant but closely adjoining the

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1985.tb00435.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Association between HLA and multiple sclerosis (MS) was investigated at the population level on 100 MS patients genotyped for HLA‐A, B, C, DR and Bf, Glo, and on 155 patients phenotyped for the same HLA antigens. Association between MS and DR2 was clearly confirmed, although its strength is rather weak. No other genetic marker could be related to the disease, no haplotype nor any allelic combination could be recognized as MS specific, and antigen genotype frequencies among the diseased could not ascertain the mode of inheritance, although dominance is very likely.Computer analysis between HLA, Bf, Glo and age of the patient, sex, age of onset and evolution of MS, impairment indexes, titres of anti‐DNA and anti‐measles antibodies in CSF did not show any interaction.Twenty sib pairs and two trios of MS were also studied; they showed no significant distortion with the random distribution of haplotypes. DR2 gene frequency, however, was significantly higher in sib pairs showing one or two haplotypes than in HLA different affected siblings. Three crossing‐overs were identified which suggest where the HLA‐linked MS susceptibility (MSS) gene could be located within the HLA segment, while other epistatic MSS genes or environmental factors are likely to be

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1985.tb00436.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Three‐factor associations in the HLA system are frequently tested statistically by well‐known procedures using 2x2x2 contingency table analysis. The coupling index DABCgiven in many publications is suitable for none of these procedures. A more meaningful statistical test for the index DABCis descri

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1985.tb00437.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

An improved method for screening human hybridoma antibodies to cell surface antigens is described. The following modifications have been developed: (1) rapid expansion of desired screening cell types by EBV transformation; (2) use of only 5 × 104cells/well; (3) elimination of the need for glutaraldehyde fixation; (4) elimination of the requirement for PLL to attach cells to microplates; (5) preparation of a large number of plates which can be stored at 4° for 3 months; (6) Protein A‐peroxidase ELISA assay yielding excellent replicates, low background “noise”, and high OD readings for positive wells. The techniques we have developed should greatly simplify and shorten the assay procedures for detecting human antibodies to a variety of cell surface a

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1985.tb00438.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Immunoglobulin allotypes have been studied in the families of 17 pauciarticular‐onset juvenile chronic arthritis patients included in the 9th Histocompatibility Workshop. There is no evidence of an association of these genetic factors with the disease, nor with the HLA and complement C4 disease marker

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1985.tb00439.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Twenty‐eight unrelated hemophilia A patients, seven of them with anti‐factor VIII antibodies were typed for HLA‐A, B, C antigens and 25 of them for HLA‐DR. The results show a significant difference in HLA‐DR4 frequency between hemophiliacs with antibodies who lack this antigen and hemophiliacs without antibodies, in whom HLA‐DR4 is increased as compared to a healthy control series. This data suggests that DR4 may be associated with a factor preventing anti‐factor VIII

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1985.tb00440.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1985.tb00441.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1985.tb00442.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1985.tb00443.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1985.tb00444.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY