摘要:

The Heterophile Transplantation Antigen (HT‐A) is a clinically important antigen which is found in some human kidneys and on the erythrocytes and in the serum of rats and some other mammals. Recent reports suggest the possibility that the anti‐HT‐A antibodies responsible for graft rejection may cross react with one or more HL‐A specificities. The ideal way to investigate this possibility would be to perform careful serological and biochemical comparisons of purified HT‐A and HL‐A. We report here the first stage of these investigations, the examination of the biochemical properties of HT‐A. The HT‐A molecule of rat plasma is apparently a glycoprotein with a molecular weight>1,000,000. The HT‐A antigenic determinant site seems to reside in the carbohydrate portion of the glycoprotein. The native HT‐A molecule is apparently susceptible to proteolytic enzymes of rat plasma and serum and is cleaved by these enzymes to produce a fragment with a lower apparent molecular weight and a larger fragment which coelutes with lipoproteins during Sepharose CL‐4B chromatography. The data presented here should allow development of a rational purification scheme for the HT‐A glycoprotein of rat plasma to be used in further studies in the relationshi

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb00033.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

The effect of blood transfusions, given in low number (<5), on the immune response of renal dialysis patients was studied. A significantly lowered response of lymphocytes to mitogen stimulation was observed in patients after as few as one, two or three transfusions, depending on the patient. This led to an increased ▵48/▵0 ratio reflected by the enhanced response of the cells following delayed addition of sub‐optimal dose of mitogen. There was no modification of the ratio of helper/inducer to suppressor/cytotoxic T cells subsets (OKT4/OKT8). The use of such simple in vitro tests in a strict protocol of transfusions could allow an adequate follow‐up thereby limiting the risks of sensitization. These results demonstrate that important phenomena affecting patients' immune response are turned on following even a low number of transfusions in the majority of uremic patients. This could probably be related to the beneficial effects of blood transfusions on the kidney allograft survival already de

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb00034.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

The frequency of HLA‐A and B antigens were studied in 32 Japanese patients with congenital hypthyroidism due to thyroid dysgenesis, and in their parents. The incidence of the Aw24 antigen was significantly higher in 27 mothers of patients with ectopic thyroid (91.3%, corrected P<0.037) and it seemed to be slightly higher in patients (77.7%) than in controls (56.8%). The Aw24 antigen was also found in 4 patients with thyroid hypoplasia and their mothers. No difference was found in the incidences of the antigen in the fathers of patients and in controls. The haplotype frequencies were not significantly different in controls, patients and their parents. These findings suggest that the gene for susceptibility to congenital hypothyroidism due to thyroid dysgenesis is closely linked to the gene for the HLA‐A locus of the patients' moth

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb00035.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

The HLA‐A, B, and C antigens of 243 individuals from two Polynesian islands, Maiao and Hiva Oa, have been characterized. 328 haplotypes were defined by family analysis. Most frequent antigens were A2, A11, Aw24, A26, Aw34; Bw38, Bw39, Bw48, Bw55, Bw56, Bw60; Cw1, Cw3, and Cw4. Several other antigens were present at low frequency or were present on only one island. New variants of Bw22 and B40 were found. Despite an overall similarity in antigens of the two islands, the combination of HLA‐A, B and C antigens on the haplotype were distinctive. Of 328 total haplotypes identified, 65 were unique. Thirteen of these (20%) were present on both islands. Twenty‐eight (43%) were unique to Hiva Oa and 24 (37%) were unique to Maiao. Significant, positive linkage disequilibrium values (▵) for HLA‐A, B and HLA‐B, C antigens were also different for the two

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb00036.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Typing for genetic variation in the second complement component C2 was performed on sera from HLA haplotyped Canadian families. Part of the data has been studied and analysed as a population; in addition there is a further random collection of haplotypes bearing the C2*2 allele. In the population data there were 444 separate haplotypes from unrelated parents or other founders: 4.7% of the haplotypes carried the uncommon allele C2*2; one haplotype carried the rare C2*3. Study of C2 2–1 heterozygotes in the population data revealed 59 haplotypes which carried the common C2*1 allele and one which carried a deficiency allele C2*0. The remaining haplotypes carried either C2* 1 or else an undetectable C2*0 allele. In the entire data there were 281 meioses informative for C2. The only recombinant between HLA‐B and C2 showed the C2 locus to be on the DR side of the B locus. Strong allelic association between C2 *2 and Bw22 and less strong association between C2 *2 and B15 suggested the possibility of two ancestral C2 mutants. Examination of other markers on these and subsequently collected haplotypes do not conflict with this idea since the B15 haplotypes mostly carry C4A *4, C4B*2 whilst the Bw22 haplotypes mostly carry C4A*4, C4B*4. The alternative idea, that there was one original mutant which crossed over from a B15 to a Bw22 haplotype or vice versa is not excluded, however. Since approximate equilibrium has been reached between Bw22 and the HLA‐A locus alleles on these C2*2 bearing haplotypes, we conclude that this mutation is at least 5000 years old.Other haplotypes carrying C2*2 are assumed to be ancestral recombinants; if this is true, the C2 locus map position between HLA‐B and HLA‐DR is confirmed. Study of C2 mutation may provide a model for understanding the genetics of some disease susceptibility genes in the H

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb00037.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Twelve Sardinian patients affected by histologically defined classic Kaposi's sarcoma (KS) were HLA‐A, B, C and DR typed.Compared to 220 age and ethnically matched healthy controls, KS patients showed a significant increase in HLA‐DR5 (66.6 vs 23.1%, P<0.001) and a considerable decrease in HLA‐DR3 (8.3 vs 53.6%, P = 0.0055).No definite association was observed for other HLA antigens. These results confirm the existence of an HLA associated genetic control of KS susceptibility and support the hypothesis that HLA‐DR5 plays the role of a predisposition marker while HLA‐DR3 bears a genetic resistance to th

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb00038.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

One hundred unrelated patients with type IIa hyperlipoproteinemia have been investigated for their HLA‐A and B antigens and compared to 171 normal controls. This study does not support the significant increase of HLA‐B17 and Bw35 previously reported by oth

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb00039.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Thirteen Danish women with postpartum thyroiditis were HLA‐A,B,C and ‐DR typed. Nine of ten unrelated probands were DR4‐positive which is significantly (corrected p = .01) different from the frequency (34.7%) of this antigen in unrelated con

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb00040.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb00041.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY