ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01755.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:: α HLA‐DR4 extended haplotypes have been established by family studies in children and adults with chronic arthritis, the children having polyarticular juvenile rheumatoid arthritis and the adults rheumatoid arthritis. These diseases are probably the same disorder presenting at different ages. The results demonstrate age‐related differences in the frequency of HLA‐DR4 extended haplotypes between the two groups of patients. The childhood onset was associated with the presence of the HLA‐B44, SC30, DR4 extended haplotype, whereas the adult form was more strongly associated with the HLA‐Bw62, SC33, DR4 haplotype. These results suggest that differences in the age at onset are influenced by the MHC in addition to the presence of the HLA DR4 g

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01756.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:: HLA‐A,B,DR antigens of two groups, one of normal individuals (N−= 31) and another of CRF (Chronic Renal Failure) patients (K−= 37), who did not develop anti‐HBs protective antibodies after Hepatitis B (HB) vaccination, were compared, respectively, to the HLA antigens of two corresponding control groups (N+=52, K+=49), who responded to the vaccine. A statistically significant difference (Pc<0.02) in the frequency of HLA‐DR3 was observed between responders and non‐responders. An increased frequency of HLA‐A1 and HLA‐B8 in N−as well as of HLA‐A1 and HLA‐B35 in K−was also noticed, but this was not of statistical significance. As these antigens have been associated to both HBs antigenemia as well as chronic active hepatitis, we suggest that these genes or other genes in linkage to those may suppress the response to HBV vaccination while, in parallel, they may predispose to an autoimm

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01757.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:: HLA‐DR,DQ specificities are determined by serological methods in 2,586 North American Caucasians. Antigen frequency, gene frequency and haplotype frequency are computed for each phenotype observed. The DR. and DQ loci antigen distributions are well‐fitted to a Hardy‐Weinberg equilibrium (p>0.25 for DR locus, p>0.10 for DQ locus). All World Health Organization (WHO) recognized HLA‐DR,DQ specificities were found except HLA‐DRwl8, which has been identified only in the black population. DR and DQ linkage disequilibria among recently defined splits is observed. The following DR and DQ associations are found: DR1 and DQw5; DR4 and DQw7, DQw8; DR7 and DQw2, DQw9; DR8 and DQw4, DQw6, DQw7; DR9 and DQw2, DQw9; DRw10 and DQw5; DRw11 and DQw6, DQw7; DRw12 and DQw5, DQw7; DRw13 and DQw6, DQw7; DRw14 and DQw5, DQW7; DRw15 and DQw6, DQw7; DRw16 and DQw5; DRw17 and DQw2. In this large study population, the following unusual DR and DQ associations are found: DR4, DQw2; DR4, DQw1; DR1, DQw7; DR7, DQw5; DRwl7, DQw6; and other unusual haplotype phenotypes containing

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01758.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:: The frequencies of HLA‐DP, DQ and DR RFLP types are compared between insulin‐dependent diabetes mellitus (IDDM) patients and healthy controls in the South Indian population. There are no significant differences in the frequencies of any of the DPA.DPB haplotypes, when allowance is made for multiple comparisons. The individual frequencies of two novel alleles, designated “DPA*B” and “DPB*B”, however, are significantly higher in controls than in patients, suggesting that these alleles are protective against IDDM. A negative association with DRwl5 (DR2).Dwl2 is also observed. The positive association with DPw3/6 RFLPs previously observed in White Australians is not present in South Indians. This difference may be due either to undetected heterogeneity within allelic classes or to different linkage disequilibrium patterns between the

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01759.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:: Informative pedigree analysis of two rare C4 allotypes is reported. One proband was C4A deficient as a consequence of having one haplotype with a deletedC4Agene, and the second haplotype with twoC4Bgenes ‐ one encoding the commonC4B*1and one encoding a unique hybrid gene product C4B*3. C4B*3 had approximately normal C4B hemolytic activity, a single α‐chain of MR94,000 by SDS‐PAGE but was positive forRg:1,2by hemagglutination inhibition (HAI) and forRg.1by Western blotting. The hybrid nature was confirmed by RFLP analysis with aRg:1‐associated fragment byEco0 109digestion but noC4A‐associated fragments byNlaIVdigestion were identified. A gene conversion at Locus I which included just the C4 isotype region could explain the structure ofC4B*3.The second pedigree had a Rodgers negative C4A*12 allotype. ThisC4Agene, which segregated with a single 7.0 kbTaqlfragment, encoded a C4A α‐chain, which was negative forRg.1epitope. The affected haplotype lacked theRg:1‐associated fragment byEco0109digestion yet had theC4AspecificNlaIVdigestion fragment. These studies successfully employed RFLP analyses to confirm serologic and electrophoret

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01760.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T‐cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, α and β. The majority of monoclonal antibodies (mAb) reactive with the human CD8 antigen bind the CD8α chain, while a single mAb, T8/2T8–5H7, has been identified which binds to the CD8 α/β heterodimer. While the two chains of CD8 have been presumed to be coordinately expressed in normal T cells, this is not always the case. Northern blot analysis of a panel of T‐cell leukemias and normal cells demonstrate that CD8α and CD8β are not invariably co‐transcribed and phenotypic analysis of fresh and interleukin 2 (IL‐2) expanded peripheral blood mononuclear cells (PBMC) confirm that the CD8α and CD8β chains are differentially expressed at the cell surface. Four distinct subpopulations of CD8 + cells have been identified based on the expression of CD8 α/α or CD8 α/β complexes: (1) T‐cell receptor (TcR) αβ+ T cells which are CD8 α+/β+ (2) TcR αβ+ T cells which are CD8 α+/β‐; (3) TcR γδ+ T cells which are CD8 α+/β‐ and (4) natural killer (NK) cells which are CD8 α+/β‐. We also demonstrate the down‐regulation of the CD8 α/β heterodimers from the surface of a CD8 + T‐cell clone following treatment with phorbol myristate acetate (PMA) while CD8 α/α homodimers remain on the cell surface. This observation demonstrates that a) a CD8+ T‐cell clone can express both CD8 α/α homodimers and CD8 α/β heterodimers and b) these two complexes do not have identical biological properties. Together, these data suggest that CD8 α/α and CD8 α/β dimers may not subserve identical functions. The differential contribution of these two CD8 complexes should be considered i

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01761.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:: Monoclonal antibody, Fel 7, produced against cat T cells, was found to react with a single‐chain glycoprotein of Mr 65,000 present on a majority of the thymocytes, 40% of lymph node cells, 15% of splenocytes and 25% of blood mononuclear cells. Using a previously reported antibody that recognizes the feline CD8 antigen, approximately 65% of cat thymocytes were shown to express both the Fel 7 and fCD8 antigens, while 14% and 6% expressed either the Fel 7 or the fCD8 determinant respectively. The Fel 7 and fCD8 antigens were expressed by mutually‐exclusive subpopulations of peripheral T cells, and not by B cells, macrophages or other types of blood cells. Expression of the Fel 7 antigen was down‐regulated and the molecule was phosphorylated when T cells were stimulated with phorbol ester, while the expression of the fCD8 antigen was unaffected by this treatment. The addition of soluble Fel 7 antibodies efficiently blocked Con A‐induced proliferation of T cells in a dose‐dependent manner. The data suggest that the mAb Fel 7 identifies a feline CD4 homologue, providing an important reagent for the study of normal and abnormal T cell developmen

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01762.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01763.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1990.tb01764.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY