摘要:

A two‐stage procedure is described for cytotoxicity tests in which three different sera from a prospective kidney recipient are pooled in each well of a standard microcytotoxicity test plate. The results are reproducible and as sensitive as those obtained with single sera tests. The technique permits a reduction in time required for a test and the number of wells that must be evaluated to screen a series of sera. It enables a laboratory to run all available sera on patients for cadaver kidney crossmatching and to eliminate hyperacute rejections. Serum pooling may also be used for screening of many sera against random cell panel

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01120.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

HLA‐typing was performed in 149 patients with essential hypertension, 86 males and 63 females. In 66 patients with significantly elevated serum levels of immunoglobulins, HLA‐B27 was increased to 18%, from 8% in the controls (P<0.007). This was not significant when correcting theP‐value for the number of antigens analyzed, but confirms reports of an association of this antigen with serum levels of immunoglobulins. HLA‐Bw15 was found to be increased two‐fold in patients with a family history of hypertension (Pcorrected<0.05) and in patients with autoantibodies (not significant). This is discussed in relation to the increase of Bw15 in juvenile diabetes and in Systemic Lupus Erythematosus, diseases in which vascular damage al

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01121.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

HLA antigens have been detected in normal human serum by inhibition of monospecific HLA antisera in the complement‐dependent cytotoxicity test. The antigens present in serum were the same as those found on the donor's lymphocytes. HLA‐A9 was unique in that it was present in serum at inhibitory titers of 1:8 to 1:32 whereas the majority of A‐ and B‐locus antigens were present at titers of 1:4 or less. Serum HLA‐A9 antigens were found in the high density lipoprotein fraction with a molecular weight greater than 200,000. In the presence of detergent they have an apparent molecular weight of 86,000 which is reduced to 46,000 when treated with papain. These values are similar to those obtained for detergent‐solubilized cellular H

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01122.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

Several normalization procedures have been applied tosingleMLC‐typing experiments in order to neutralize experimental variation and hence to secure assignment of HLA‐D phenotypes. The present paper concerns a statistical description and isolation of experimental and biological factors inrepeatedMLC‐typing experiments. The statistical model used gives a meaningful description of the variables in MLC as judged by its application to MLC‐typing data of HLA‐identical siblings. Subsequent testing of the model in an HLA‐A, ‐B, ‐C and ‐D‐genotyped family material provides evidence that the level of typing response to selected homozygous typing cells may be influenced by factors coded for by the homologous HLA‐D region. It is, however, concluded that such evidence must be accepted with caution due to the (for the present statistical purpose) suboptimal design for the HLA‐D typing experiments and hence the relative insensitivity of the method. A general method for locating erroneous

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01123.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

The relationship between the major histocompatibility complex (MHC) and functional receptors on the surface of human lymphocytes was studied. HLA antisera were tested for their effect on the formation of E‐, EA‐, and EAC'‐rosettes by human peripheral blood lymphocytes (PBL). Antisera to various HLA specificities inhibited the formation of EAC'‐rosettes, but had no effect on the formation of E‐rosettes. The formation of EA‐rosettes was inhibited by HLA antisera only in part among the individuals tested. Anti β2‐microglobulin serum resembled HLA antisera in its effect on the formation of the various rosettes.HLA determinants and complement receptors are different entities on the cell surface since elimination of complement receptors by trypsin treatment does not seem to affect the expression of HLA antigens on the cell surface. It is suggested that EAC’receptors are located close to

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01124.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

B lymphocytes were isolated from fresh blood and frozen‐thawed lymphocyte preparations from the same donors. A panel of 55 human antisera, previously found to react with human peripheral blood B lymphocytes in cytotoxicity assays, was screened against these B‐cell preparations. There was essentially no difference in the reactions of B‐cell antisera with fresh B lymphocytes when compared with frozen‐thawed cells. The percentage recovery of B cells from the fresh and frozen preparations was not significantly di

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01125.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01126.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

In 22 cases of myxedema 22 HLA‐A and B antigens were typed. In six out of eight patients with a history of subacute thyroiditis Bw35 antigen was demonstrated, while in none of 14 patients free from this disease in anamnesis could this antigen be found. This difference is statistically significant, and suggests classifying of myxedema patients into two groups according to pathogenesis of the diseas

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01127.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1977.tb01128.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY