摘要:

Iodination with lactoperoxidaae ‐125I‐ ‐ H2O2was used to label surface components of rat epidermal cells. Lysis of the cells in non‐idet P40resulted in the solubilization of tissue‐specific antigens and of concanavalin A receptors. These specificities were demonstrated using a radio‐immunoprecipitation method. The tissue‐specific antigens were recognized using absorbed rabbit anti‐rat epidermal cell sera (Lloyd&Darnule 1974); they reacted with two low molecular weight components in the lysate (9,000 and 12,000 daltons). Conca‐navalin A reacted with three major components. Two had high molecular weights (75,000 and 95,000 daltons). The possibility that one of these components was radioiodinated lacto‐operoxidase, which would have reacted with concanavalin A, was disproved. Another component (which occasionally appeared as two peaks) was similar in size to the species detected by the tissue‐specific antisera. Their non‐identity was, however, demonstrated by the finding that thr two specificities could be precipitated inde

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1975.tb00631.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

The finding that the SD antigen HL‐A8 is associated with myasthenia gravis has raised the question as to whether or not there is also an association between some specific LD antigen and myasthenia gravis andor HL‐A8.MLC technique was used to study LD antigens in 33 myasthenic patients. The cells of a myasthenic patient who was homozygous for both HL‐A and LD products were used in MLC tests as stimulators with HL‐A8 bearing cells from 24 myasthenics, their 17 relatives and 16 controls and to non‐HL‐A8 cells from nine myasthenics and 16 controls.At least three different LD genes were found to be associated with HL‐A8. One of them, called LDm, was present in 17 (63 %) of the 27 HL‐A8‐bearing haplotypes of myasthenics, and in nine (47 %) of the 19 HL‐A8‐bearing haplotypes of controls. In our study LDm, was not found in the 84 haplotypes devoid of HL‐A8.LDm, is strongly associated with HL‐A8 and through this also with myasthenia. Calculated from phenotype frequencies for HL‐A8 and its association to LDm, the LDm, frequencies are 9 % in the control population, 30 % in myasthenics and 48% in young females with onset of myasthenia below35years.LDmhad no independent correlation with any of the clinical parameters of myasthenia gravis, even though is was found more often in HL‐A8+ females with the onset of my‐asthenia before 35 years than in the whole myasthenic group. LDmappear

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1975.tb00632.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Human epidermal cells dissociated by repeated trypsinization of skin explants were stimulatory to allogeneic lymphocytes in mixed lymphocyte skin‐cell cultures. After elimination of the lymphocytes proliferating in response to a particular allogeneic lymphocyte donor by a “hot” pulse of3H‐thymidine of high specific activity, the viable lymphocytes remaining in culture were still capable of responding to stimulation by epidermal cells from the same donor. The response towards allogeneic epidermal cells could only partially be eliminated by3H‐thymidine treatment. This treatment, however, also partially eliminated the secondary stimulation response towards lymphocytes from the epidermal cell donor used initially, but did not alter the response to lymphoid or epidermal cells from an unrelated third party donor. In addition, HL‐A antisera, which specifically inhibited the stimulatory capacity of mitomycin‐treated lymphocytes that carried the relevant HL‐A antigen also inhibited the stimulatory properties of epidermal cells from

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1975.tb00633.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Rhesus monkey lymphocytotoxic alloantisera were tested in437random unrelated monkeys and in membersof19 pedigreed families. Groups of sera or individual sera identified 11 specificities. Genetic analysis of the associations of these antigens revealed behavior consistent with the current concept of the major histocompatibility complex of several mammalian species including man in that the antigens could be grouped into two series of closely linked co‐dominantly expressed allele

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1975.tb00634.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

In general it can be said that the HL‐A (LAand FOUR) antigens seem to be of major qualitative and partly quantitative importance for the degree of destruction in Cell Mediated Lymphoiysis (CML).This has been established by analyses of 1299 effector/target combinations involving 97 different healthy individuals.The main observation is that the cytolytic capacity of MLC effectors is positively ranked with the number of HL‐A (LAand FOUR) antigens introduced by the incompatibility between stimulator, effector and target lymphocytes. However, the fact that not all cytolytic situations can be explained by the HL‐A system stresses that other specific antigens (Target Determinants) may interfere with the Cell Mediated Lympho

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1975.tb00635.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

The gene products of the LA and FOUR loci of the human Major Histocompatibility Complex (MHC) are generally considered to be a major target in the Indirect Cell Mediated Lympholysis (ICML) test. Within most experiments, a positive correlation exists between the number of HL‐A antigens challenged and lympholysis. When different experiments are collated this correlation is less obvious. This discrepancy might be caused by differences between the individual HL‐A antigens involved in the afferent phase (MLC) andor the efferent phase (CML).In28experiments involving97unrelated individuals we have compared statistically 12 different antigens governed by the LA and FOUR loci.When only one of these antigens is challenged in ICML, target lymphocytes are lysed to different degrees allowing a significant classification of the antigens into different groups, which do not coincide with the classification in the LA and FOUR series antigens.It is concluded that the antigens of the HL‐A system are not of equal importance when challenged separately in ICML. The existence of a separate CML locus and a corresponding linkage disequilibrium to the SD loci of the MHC region is sugg

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1975.tb00636.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

The Indirect Cell Mediated Lympholysis (ICML) test has become a reliablein uitrotool in investigations of the immunogenetic background of the cellular immune response. It is well established that the antigens of the LA and FOUR loci of the human Major Histocompati bility Complex (MHC) or antigens governed by closely linked loci exhibiting a marked linkage disequilibrium are of major qualitative and quantitative importance in ICML.This communication is concerned with the importance of the HL‐A third series antigen AJ(W20)on lympholysis in ICML. From investigations of47combinations where only the AJ (W20) antigen may be attacked, it is concluded that this antigen is, in itself, a poor ICML target determinant, but thatAJ(W20) may function as a stronger ICML target determinant in concert with a FOUR antigen, although the actual FOUR antigen cannot be attacke

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1975.tb00637.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

One hundred % of 66 patients with pelvospondylitis were found to haveHL‐A27, whereas the frequency of the antigen in 60 patients with sacroiliitis was 70 %. The difference in frequency ofHL‐A27between the two patient groups is statistically highly significant. This implies that tissue typing could be of clinical importance for a prognostic evaluation of patients with sacroiliitis.Studies in families with severalHL‐NMLCidentical sibs indicated a relatively low penetrance of disease in those carrying the gene for increased susceptibility to development of AS. No assocation ofASto particular MLC alleles was found either in families or in severalSD‐2identicalASp

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1975.tb00638.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

In a prospective study, sixty‐five histologically verified Swedish cases of Hodgkin's disease were HL‐A typed. The antigen frequencies in the patient group did not deviate from a group of normal controls. The frequency of HL‐A8 was slightly increased in patients over 40 years (P<0.05). HL‐A12 was markedly increased in patients below 40 years with a favourable histopathology (P<0.001). No association between sex or clinical stage and distribution of HL‐A phenotypes was observed.The number of T‐lymphocytes in blood and the spontaneous DNA synthesis and DNA synthesis induced by concanavalin A, pokeweed mitogen, phytohemagglutinin and PPD antigen in blood lymphocytesin vitrowere determined in 60 patients. A significant increase of HL‐A28 was noted in patients with advanced disease (P<0.01) and in patients responding poorly to PPD (P<0.001) and PWM (P<0.01). No correlation between HL‐A antigen distribution and T‐lymphocyte co

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1975.tb00639.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

We have shown in retrospective and prospective studies that there is an increased frequency of HL‐A1 in subjects with recrudescent circum‐oral herpes when compared with the frequency in the background population. This is the first association of a human disease of known viral aetiology with a specific transplantation antigen, and it is possible that this may represent a marker for an immune response g

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1975.tb00640.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY