ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb00376.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Statistical features of the method of antigen genotype frequencies among the diseased, for single and multiple disease associations at a locus, will be presented. A methodology to determine when a true intermediate mode of inheritance can be distinguished from strict recessive or additive inheritance will be developed. The effect of sporadics and ascertainment bias on the observed antigen genotype frequencies will be investigated. Data on ankylosing spondylitis, multiple sclerosis and dermatitis herpetiformis are very close to expectations for an additive (or dominant) mode of inheritance for the HLA‐linked disease‐predisposing gene, and data on hemochromatosis, insulin dependent diabetes mellitus and celiac disease are close to recessive expectations. If an intermediate model does apply in any of these cases, it must be an intermediate model that is fairly close to a strict recessive or dominant model, as appropriate. DR data for insulin dependent diabetes mellitus (IDDM) strongly indicate that there are two separate “disease” alleles, which exhibit negative complementation, predisposing individuals to IDDM, where the mode of inheritance of the “disease” alleles considered separately is close to recessive. In general, this method cannot rule out the existence of sporadics or a second disease‐predisposing gene, when the penetrance values over the two disease‐predisposing genes are strictly additive, for diseases showing agreement with additive (or dominant) modes

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb00377.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

In an attempt to study the gene products of theHLAcomplex, a monoclonal antibody, named HU‐30, was produced by immunizing BALB/c mice with a cultured human B lymphoblastoid cell line, Shi‐C3 (Aw24, Aw31, Bw51, Bw52, DR2, DR blank, MT1, MT2, MB3). HU‐30 belonged to the IgG2 subclass and was active in complement dependent cytotoxicity. When the serological specificity was evaluated with a panel of 15 cultured human lymphoblastoid cell lines, it was found that HU‐30 detected a polymorphic determinant, common to HLA‐DR1 and 2, with much stronger cytotoxic activity against HLA‐DR2 positive B cell lines. When HU‐30 was tested against a panel of B cells from 84 healthy donors at a dilution of 2‐13, it gave positive reactions only with cells typed as HLA‐DR2. Furthermore, sequential coprecipitation studies indicated that the HU‐30 determinant was borne on the molecules carrying the HLA‐DR determinants. Thus, HU‐30 appears to be of great value as a tissue typing reagent m

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb00378.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

A method is described which permits culture of both arterial and venous canine endothelial cells. Cell‐mediated cytotoxicity against cultured endothelial cells has been studied. A51Cr‐release assay was used to detect CTL generated in MLC. Both arterial and venous endothelial cells are lysed by CTL specifically. Cold target inhibition experiments have been performed to analyse the CTL‐recognized antigens on arterial and venous endothelial cells. Different antigens are recognized by CTL on venous endothelial cells and PHA‐blasts; it is possible that CTL recognize venous endothelial cells through class II antigens or E‐M antigens. Arterial endothelial cells and PHA‐blasts share CTL‐recogn

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb00379.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

In a search for genetic recombinations within the major histocompatibility complex (MHC) of the chicken, theB‐complex, the offspring from matings between heterozygousB15/B21andB4/B6animals were analysed by red cell agglutination. Among the progeny, 8,912 informative typings were performed. Four recombinants were found, all separating theB‐complex lociB‐FandB‐G(B‐Fcodes for Class I antigens,B‐Gcodes for an antigen of which there is no known homologue in mammals).B‐L(Class II antigen) always followedB‐F. Stimulation in graft versus host reactions and in mixed lymphocyte cultures followedB‐F/B‐L.The mapping distance between the two lociB‐FandB‐Gis in the range of 0.04 centimorgan. The lack of recombinants separating individualB‐Floci in this study and in the studies of others might indicate that chickenMHCis less complex than those of mammalian species, but alternative explanations are also possible. So far no serologically defined recombinant separating Class I (B‐F) and Class I

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb00380.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Human amniotic fluid cells, known to express HLA–A, –B, and –C antigens, were tested for the presence of lymphocyte‐stimulating antigens (LD or HLA–D) using modifications of the mixed lymphocyte culture (MLC) and primed lymphocyte typing (PLT) tests. Peripheral blood lymphocytes were co‐cultured with various concentrations of allogeneic amniotic fluid cells, either growing as a monolayer culture in microtiter plates or suspended in medium following treatment with trypsin. The kinetics of such mixed lymphocyte amniotic fluid cell culture (MLAC) reactions were followed during days 3 to 8. Under none of these conditions did amniotic fluid cells significantly stimulate allogeneic lymphocytes, even after lymphocytes were specifically primed in the PLT assay to the HLA–D antigens segregating in the family of the amniotic fluid cell donor. Furthermore, in three‐cell experiments, amniotic fluid cells failed to inhibit an ongoing MLC reaction, indicating that the absence of proliferative response to amniotic fluid cells is not due to active suppression. Taken together, these data strongly suggest that amniotic fluid cells either do not express HLA–D antigens or do not express them in a form that is detectable in either primary

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb00381.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The specificity of the inhibitory activity of anti HLA‐DR antisera on the proliferative response of human T cells to soluble antigens has been recently challenged. Furthermore, there are conflicting reports about the effect of antisera to the stimulatory antigen. Therefore, we investigated the inhibitory activity of different antisera on the proliferative response of T lymphocytes from sensitized donors to the antigens HSV and PPD. Alloantisera to DR specificities shared between antigen presenting cells (APC) and T cells displayed a strong inhibitory activity; alloantisera to HLA‐DR specificities expressed only by APC or the T cell donor displayed lower or no inhibitory activity. Monoclonal antibodies to monomorphic determinants of HLA‐DR molecules were inhibitory, but only when used at a high concentration. Antisera to HLA‐ABC molecules and to HSV displayed little if any inhibition. These findings provide further evidence that the HLA‐DR molecules as such in the APC membrane, which were also present during initial sensitization, are restriction elements fo

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb00382.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb00383.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb00384.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1983.tb00385.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY