摘要:

Cytotoxic T lymphocytes were activated in primary one‐way mixed lymphocyte cultures of cells matched for serologically defined HLA‐A, ‐B and ‐C antigens. In 16 out of the 29 combinations mismatched for the HLA‐D/DR antigens, cell‐mediated lympholysis of the stimulator cells occurred. The specificity of 5 selected cytotoxic T lymphocytes was studied in detail. Three of these cytotoxic T lymphocytes recognize antigenic determinants associated with HLA‐Bw35 (Breuning et al. 1984, II). The 2 other cytotoxic T lymphocytes failed to lyse T‐target cells enriched by resetting with sheep red blood cells, whereas target cells from the ‘non‐T’fraction were strongly lysed, indicating that antigenic determinants associated with Class‐II HLA molecules were the targets recognized by these cytotoxic T lymphocytes. This notion was supported by a study of a panel of HLA‐typed third‐party target cells. One cytotoxic T‐lymphocyte population preferentially lysed HLA‐DR2‐positive target cells. Family studies, including a family with a recombination between HLA‐B and ‐D, showed that the target antigen recognized by the latter cytotoxic T lymphocyte segregated with DR2. The second cytotoxic T‐lymphocyte population recognized a determinant associated with DRw8. However, in 13 of the 29 HLA‐A‐, ‐B‐ and ‐C‐identical, D/DR‐different combinations, cell‐mediated lympholysis of stimulator target cells could not be detected, not even on enriched ‘non‐T’target cells. Thus, after primary mixed lymphocyte culture of HLA‐A‐, ‐B‐and C‐identical, HLA‐D/DR‐non‐identical cells, cytotoxic T lymphocytes directed against sensitizi

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb02110.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

We compared five cytotoxic T lymphocytes raised by primary mixed lymphocyte cultures of HLA‐A, ‐B and ‐C serologically identical Bw35‐positive responder‐stimulator combinations. When tested on a panel of third‐party target cells, the reactivity pattern of these cytotoxic T lymphocytes allowed the distinction of three subtypes of HLA‐Bw35. Cold‐target inhibition experiments and analysis of CTL activity at the clonal level showed the existence of subsets of CTLs directed against distinct antigenic determinants associate

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb02111.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

A study was performed to evaluate the association of HLA‐DR antigens with the proliferative response of T cells in autologous mixed lymphocyte cultures. Peripheral blood mononuclear cells from 100 normal healthy individuals were typed for HLA‐DR antigens and autologous mixed lymphocyte cultures were established. A low proliferative response from autologous cultures was found with individuals bearing HLA‐DR3 antigens and in individuals with only one identifiable HLA‐DR antigen. In contrast, a strong proliferative response was associated with HLA‐DR6 and two identifiable HLA‐DR antigens. These data are consistent with the hypothesis that HLA‐DR3 antigens are associated with a weak immune response gene and HLA‐DR6 antigens are associated with a strong immun

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb02112.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

XI 20.4 monoclonal antibody belongs to the IgM class. It precipitates two polypeptide chains characteristic of HLA Class I antigens. At the highest dilutions it is cytotoxic against lymphocytes carrying antigens of the HLA‐A3 crossreacting group. Lysostrip experiments show that, at the lowest dilutions, the antibody reacts either with HLA‐A, or B antig

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb02113.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

In the present study we have investigated the HLA‐SB antigen distribution in 65 unrelated Israeli Ashkenazim and non‐Ashkenazim and in a panel of 18 local homozygous typing cells (HTC). Two locally derived SB reagents, anti‐SB2 and SB3, were also studied. The HLA‐SB allele frequencies in the Israeli sample ranged from 0.02 for SB1 to 0.47 for SB4 while SB5 was absent. SB3 had a higher frequency in non‐Ashkenazim (0.11) as compared to Ashkenazim (0.06) but this difference was not significant. On the whole, the allele frequencies for the 5 HLA‐SB antigens studied were in the range observed in non‐Jewish Caucasoid populations with some minor variations. Two of eighteen local HTC's were found heterozygous for SB, demonstrating that homozygosity for HLA‐A, B, C, D and DR does not indicate homozygosity for HLA‐SB. The local anti‐SB2 and SB3 typing reagents gave concordant results when compared with the NIH reference typing cells in

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb02114.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb02115.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1984.tb02116.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY