摘要:

The distribution of transferrin receptor (TfR) positive cells and their staining intensity were examined in the liver, duodenum, pancreas, spleen, kidney and brain of iron deficient, iron‐overloaded and normal Wistar rats to elucidate the regulatory effects of iron on TfR expressionin vivo.Iron deficiency was produced by an iron deficient food and water regimen, and iron overload by repeated intraperitoneal injections of ferric nitrilotriacetate (Fe3‐NTA) for 12 weeks. In iron deficient rats, levels of hemoglobin (Hb= 5.9 ± 0.7) and serum iron (Sl = 29.9 ± 4.4) were lower, and total iron binding capacity (TIBC = 624.4 ± 72.7) was higher than in normal rats (Hb = 15.6 ± 0.9, Sl = 206.5 # 20.5, TIBC = 416.0 ± 56.0), and Weeversafor SI (217.7 ± 15.5) and TIBC (307.1 ± M45.4) in iron‐overloaded rats. In normal rats, TfR positive granules were observed in hepatocytes and Kupffer cells of the liver, absorptive epithelium of the duodenum, acinar and Langerhans islet cells of the pancreas, macrophages and red pulp erythro‐blasts of the spleen, and distal convoluted tubular epithelium of the kidney. Although the tissue distribution pattern of TfR positive cells was similar in normal, iron deficient and iron overloaded rats, the staining intensity and number of TfR positive cells were obviously higher in iron deficient, and lower in iron overloaded rats. We conclude that TfR expression is negatively regulated by the tissue concentration of iron. Acta Pathol Jpn 39:

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02427.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Localization of pepsinogens I and II mRNA in the human gastric mucosa was investigated by anin situhybridization method using digoxigenin labeled cDNA probes. Gastric fundic mucosa from healthy volunteers, which was stained with digoxigenin labeled pepsinogens I and II cDNA probes, showed positive staining in the cytoplasm of both chief cells and mucous neck cells. In contrast, gastric antral mucosa stained with the pepsinogen I cDNA probe showed no positive reaction in the surface mucous cells or pyloric glands. On the other hand, the pyloric glands were stained positively with the pepsinogen II cDNA probe and the staining appeared to be identical to that obtained with the anti pepsinogens I and II monoclonal antibodies using the avidin biotin peroxidase complex technique. These results are consistent with those of previous studies that have employed immunochemical and immunohistochemical techniques. Acta Pathol Jpn 39: 765 771, 1989.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02428.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Nonspecific cross reacting antigen (NCA), a constituent of the carcinoembryonic antigen family, was localized ultra‐structurally in a human lung adenocarcinoma cell line, PC 9. NCA was distributed predominantly on the plasma membrane in the early phases of cell culture. Deletion of fetal bovine serum (FBS) from the culture medium suppressed cell division without significantly altering cell viability, and induced a dramatic but reversible change in NCA localization. Under these conditions, NCA was localized to membrane degradation products within cytoplasmic vesicles and vacuoles. Acid phosphatase activity was also present in some of these intracellular structures. Similar changes in NCA localization were seen in cells cultured with FBS at day 6 when the cells reached a plateau stage of growth. These findings strongly suggest that plasma membrane degradation is accelerated by the cessation of cell growth. Cytoplasmic reactivity for NCA in cancer cells may therefore reflect degradation of plasma membrane‐associated NCA and may not necessarily be correlated with increased systhesis of this glycoprotein. Acta Pathol Jpn 39: 772 778, 1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02429.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Muscle fiber disarray which developed in the papillary muscle released from its chordal attachment after mitral valve replacement (MVR) was analysed histometrically in 23 hearts. The degree of disarray, or how far the fiber arrangement deviated from an ideal condition in which the fibers are considered to be aligned in parallel, was evaluated in terms of the “disarray index”. This index corresponds to the ratio of the minor to major axes of a regression ellipse which can be obtained histometrically by plotting the reciprocal of the number of muscle fibers intersected by a test line of equal length in every direction on a polar coordinate system. The index can vary continuously in a range from 0.00 for a completely parallel fiber alignment to 1.00 for a completely random distribution. The estimated index showed a gradual increase from 0.05 to 0.68 according to the postoperative survival period after MVR with a significant positive correlation (r=0.793, p<0.01). The index was inversely correlated with the mean muscle fiber diameter (r=‐0.433, p<0.05). These results indicate that the disarray which develops in the papillary muscle after MVR is a deformation of the normally parallel arrangement of fibers toward an isotropic texture under focal disruption of the normally longitudinally oriented tensile force. Acta Pathol Jpn 39: 779‐78

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02430.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

To clarify the relationship between the extent of the intraductal component and the invasive component in cases of invasive ductal carcinoma (IDC) of the breast, we divided 87 such cases into two groups, Group I in which the intraductal component extended for less than 10 mm (36 cases) and Group II in which the intraductal component extended for 10 mm or more (51 cases). On histological slides, there was an association between the extent of the intraductal component and the pattern of invasion. The majority (80.6%) of Group I IDC cases showed an invasive component composed of one invasive nodule, whereas 62.7% of Group II IDC cases showed an invasive component composed of two or more such nodules. On gross and microscopical examination, Group II IDC showed a significantly larger mean tumor size than Group I IDC (23.4 ± 8.9 mm vs. 18.3 ± 6.6 mm, P<0.002&24.1 ± 14.1 mm vs. 17.1 ± 6.5 mm, P<0.002). A similar result was obtained by clinical examination (41.8+17.0 mm vs. 29.5 ± 11.6 mm, P 0.0001). These results suggest that Group II ductal carcinomas may frequently develop multiple stromal invasion, resulting suddenly in a sizeable breast mass perceived by the patient. Acta Pathol Jpn 39: 786‐794

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02431.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

A small round cell tumor of soft tissue arising in the retroperitoneum of an 18‐year old woman is reported. The light and electron microscopic features of the tumor were mostly indistinguishable from Ewing's sarcoma of the bone and in some parts from differentiating neuroblastoma with mature neurons and Schwann cells. An immunohisto‐chemical study using anti NSE antibody showed many widespread NSE‐positive cells lying singly and, more often, in clusters including the undifferentiated areas. The tumor was not anatomically related to the paravertebral sympathetic ganglia or adrenal glands. The present findings strongly suggest that certain extraskeletal Ewing's sarcomas are very primitive neuroectodermal tumors. Acta Pathol Jpn 39: 795‐80

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02432.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

A unique case of embryonal rhabdomyosarcoma arising at the left pleura of a 7‐year‐old Japanese girl is reported. The present case was characterized by persistent pleural effusion, and the malignant cells incidentally found in it were the first diagnostic clue. The tumor showed a rare growth pattern involving diffuse thickening of the parietal pleura. Biopsy of the thickened parietal pleura upon thoracotomy revealed embryonal rhabdomyosarcoma largely composed of immature mesenchymal cells. Immunohistochemical demonstration of creatinine phos‐phokinase MM was most helpful among several types of immunostain for the histopathological diagnosis. Ultra‐structurally, thin filaments with primitive Z bands could be seen in some tumor cells. Intensive clinical examination revealed only diffuse thickening of the parietal pleura, which was reduced by chemotherapy. This is the first documented case of rhabdomyosarcoma arising at the pleura. Previous reports of rhabdomyosarcoma arising at unusual sites are reviewed and the histogenesis of this tumor is briefly discussed. Acta Pathol Jpn 39: 803 80

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02433.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

A fibrous pseudotumor of the testis from a 46 year old man was examined histologically and immunohisto‐chemically. The tumor, situated in the tunica vaginalis, appeared as multiple round nodules up to 2 cm in diameter, and was composed largely of fibrous tissue with scanty inflammatory cell infiltration. Besides mesothelial cells covering part of the tumor, foci of piled up cells were found among the fibrous tumor tissue. The cells in the foci were spindle and polygonal in shape, and contained oval nuclei. Immunohistochemically, these cells were positive for vimentin and keratin, but negative for CEA. Similar features have been found in pleural mesothelioma, supporting the concept that testicular fibrous pseudotumor could represent neoplastic growth of the mesothelium of the tunica vaginalis. Acta Pathol Jpn 39: 810813, 198

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02434.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

An autopsy case of primary fibrosarcoma occurring in the liver of a 75‐year old female is presented. The tumor, arising from the lateral upper portion of the right lobe, measured 14 cm in greatest dimension. Light microscopy of the tumor revealed homogeneous, spindle‐shaped cells with abundant collagen fibers showing a classic herringbone pattern. Immunoreactive vimentin was found in the tumor cells. Electron microscopically, the tumor cells were rich in rough endoplasmic reticulum without a basement membrane, and were surrounded by large amounts of collagen fibers. The fibroblastic character of the tumor cells was suggested by light and electron microscopy. This may be the first reported case of primary hepatic fibrosarcoma examined by immunohistochemistry and electron microscopy. Acta Pathol Jpn 39: 814 820, 1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02435.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Two autopsy cases of giant cell arteritis with aortic dissection are reported. One patient was an 87 year old man, and the other was an 80 year old woman. In both cases the patient died suddenly, and fatal pericardial bleeding due to the aortic dissection was revealed at autopsy. Histologically, marked inflammation with giant cells was observed in the wall of the aorta and in other arteries including the cranial arteries. The authors therefore consider that giant cell arteritis was one of the preceding lesions that culminated in aortic dissection. Acta Pathol Jpn 39: 821 826, 1989.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02436.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY