摘要:

Actomyosin‐containing cells in both non‐neoplastic and neoplastic tissues of the salivary gland, lung, breast and some other organs were studied by immunofluorescent microscopy using antiactomyosin rabbit serum. In the breast, myoepithelial‐like cells with positive immunofluorescence in the cytoplasm were observed not only in sclerosing adenosis and fibroadenoma but also in scirrhousgand medullary‐tubular duct carcinomas. No positive cells were observed in medullary carcinomas with lymphoid infiltration. The actomyosin positive cells were also seen at the outer layer of tubules of “mixed tumors” and of cell nests in adenoid cystic carcinoma and in myoepithelioma of the salivary gland, but not in the metaplastic squamous cells or in the cells of myxomatous and chondroid areas of “mixed tumor”. In carcinoma of the lung, actomyosin‐positive cells were observed in adenoid cystic carcinomas and adenocarcinoma of the bronchial gland type, but they were not seen in squamous cell carcinomas or papillary adenocarcinomas.It was concluded that the actomysoin‐containing cells with structural appearances of myoepithelial cells in a variety of tumors were neoplastic

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1978.tb01260.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Bone marrow cells were transferred into 700 RX‐irradiated syngeneic mice, and reganeration of the thymus was demonstrated by determining the total amount of DNA in the thymus. The amount of DNA in the regenerated thymus 9 days after X‐irradiation paralleled the number of cells injected, the range being 0 to 106cells. These results indicate that regeneration during this period reflects the proliferation of thymocyte precursors existing in bone marrow cells, therefore such can be used as an indicator to determine the number of thymocyte precursors.Thymocyte precursors were separated from spleen colony forming cells and soft agar colony forming cells by one G velocity sedimentation. Thymocyte precursors (more than 4.3 mm/hour sedimentation rate) sedimented faster than did spleen colony forming cells (peak at about 3.0 mm/hour) and Thy‐1 antieen was already evidenced on the cell surface as demonstrated by complement dependent cytotoxicity. Soft agar colony forming cells also sedimented faster, suggesting that cells destined to become one type of cell are larger than multi‐potential ste

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1978.tb01261.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Domestic rabbits were used to study the changes that occur during ischaemic conditions in the kidney. With a scanning electron microscope, the microcellular changes at the surface of glomerular epithelial cells were observed regularly from the onset of ischaemia until five hours later. After one hour of ischaemla, the surface of the glomerular epithelial cells showed mild swelling and some change in its smooth appearance. One part had taken on a sponge‐like appearance. After 2.5 hours of ischaemia, the epithelial cells had atrophied and the entire cell surface was clearly sponge‐like. It was no longer possible to distinguish the small pore‐like structures that had been seen on the cell surface in the normal. Moreover, 2.5 hours of ischaemia was the time when, biochemically, cell metabolism had completely ceased and the permeability of the cell membrane had altered. The cell was considered to have undergone irreversible change by this stage. After 5 hours of ischaemia, cells were markedly atrophied and the appearance of the surface had become even more sponge

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1978.tb01262.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

A freeze‐fracture replication study was undertaken to clarify the relationship between intracellular lamellar bodies of type II alveolar epithelial cells and intra‐alveolar contents in late gestation rabbit fetuses. The interior of the inclusion bodies was composed of multiple stacks and/or whorls of membranes devoid of membrane‐associated particles, while the limiting membrane of intracellular lamellar inclusion bodies was studded with membrane‐associated particles of 150Å diameter.The intra‐alveolar contents were comprised of two components; spherical bodies, and tubular elements. Spherical bodies were identical to the internal contents of the lamellar bodies and also were devoid of membrane‐associated particles. Tubular elements mostly appeared rectangular on cross‐fractured faces, but triangular and hexagonal forms could also be seen. These tubules rested on the surfaces of the spherical bodies and appeared to be formed of the outer lipid monolayer of spherical bodies. The present observations suggest that the tubular element of the alveolar contents is formed through the interaction between the discharged lamellar body content and the

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1978.tb01263.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

The cutaneous nodules obtained from seven patients with von Reckling‐hausen's neurofibromatosis were investigated by electron microscopy, and ultrastructural localization of acetylcholinesterase activity was demonstrated in the nerve fibers of this tumor for the first time using Karnovsky's thiocholine method. The enzymatic activity was mainly found in unmyelinated fibers, exactly associated with their axonal membranes, the interspace between the apposing axonal and Schwann cell membrane, and some different mesaxons, which indicated their cholinergic nature. Almost all myelinated fibers and some unmyelinated fibers did not possess the activity. The relationship between axon and Schwann cell was quite similar to that of normal peripheral nervous system, but two striking alterations of the nerves existed: One is the dissociation of unmyelinated fibers, and the other is the degenerative changes of the axon and the myelin sheath. As the evidence of schwannian proliferation, onion bulb formations and collagen pockets were observed. Some signs of fibroblastic proliferation were also found.From the present study and the review of the literature, the probable histogenesis of this disease was discusse

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1978.tb01264.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Focusing on hematopoiesis, especially granulocytopoiesis, livers from ten human fetuses between 7 to 13 weeks of gestation were observed with light and electron microscpe. In the hepatic mesenchymal tissue variable number of granulocytes in varying stage of maturation were found from 10 to 13 weeks of gestation. The granules of neutrophils in human fetal livers were classified into three distinct types. The first granules appeared in myeloblasts. The second granules, possibly formed in Golgi complex, were more larger and observed after the promyelocytic stage. The third granules, predominant in mature neutrophils, were pleomorphic. Other than granulo‐cytic, erythrocytic and stromal cells, unclassifiable cells were occasionally encountered. The role of mesenchymal tissues in the granulocytopoiesis, the development of fetal neutrophfls and the problem of hematopoietic stem cells were discusse

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1978.tb01265.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Ten autopsy cases of congenital cystic kidneys were studied by histopathological examination. Eight cases with dysplastic type revealed immature mesoderm, cartilage, localized tight fibrous tissue, hemangiomatous lesion consisting of arterlo‐capillary‐venous elements, extramedullary hematopoiesis, primitive lymph nodes, adipose tissue, and abundant neural tissue in the kidney as well as cystic lesions. These findings strongly suggest that congenital cystic kidneys of dysplastic type are derived from hamartoma or hamartomatous changes chiefly consisting of mesodermal tissue, most of which corresponds to tissue anomaly and is very close to the aberrant form of Wilms’tumor. The presence of focal fibrosis and lymphoid cell infiltration forming focal renal tissue destruction suggest local disturbance of tissue dmerentiation due to some causal elements. As common feature of this type, it is revealed that the babies show lower weights for their fetal ages and die shortly after birth or are delivered as still birth due to hypoplasia and atelectasis of the pulmonary t

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1978.tb01266.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Rhabdomyosarcomas induced by single intramuscular injections of nickel sulphide (Ni3S2) in Fischer and Hooded rats were culturedin vivoand andin vitroto study their growth characteristics and chromosomal constitution. The tumor cell suspensions culturedin vitroexhibited more myogenic differentiation on the coverslips than those cells grownin vivoin diffusion chambers. A characteristic feature ofin vivocultures was the appearance of microclusters which resembled the primary tumors.Chromosome analyses of primary tumors revealed that a majority of these had a modal number in the diploid or near diploid range. Fischer rat primary tumor cells exhibited abnormal configurations including rings, dicentrics and triradials. A comparison of the chromosome make‐up of the primary tumors and their metastases was performed on four sets of tumors. Three out of four metastases examined showed the diploid chromosome make‐up characteristic of the primary tumors suggesting that the tumors with the diploid or near diploid chromosome constitution are more likely to produce metasta

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1978.tb01267.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Brain tumors were induced in Syrian hamsters by intracerebral inoculation of brain cells which were obtained from 12‐day old syngeneic hamster and infected with Schmidt‐Ruppin strain of Rous sarcoma virus (SR‐RSV) in vitro. A total of 212 tumors were developed in all 25 recipients within 19 to 125 days alter transplantation. On the basis of light and electron microscopic study, they were classified into four main groups: astrocytoma (45.8%). pleomorphic glioma (50.0%), sarcoma (3.8%). unclassified (0.4%). The morphological features of these tumors were described, and the advantages of this brain tumor model were disc

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1978.tb01268.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

Fetal autopsy case of xeroderma pigmentosum was reported. This male fetus of 24 gestational weeks was prenatally diagnosed as xeroderma pigmentosum by detecting the DNA‐repair defect of the amniotic fluid cells. Autopsy revealed not only maldevelopment of the fetus (stillbirth) in general for the standard, but also showed slight developmental retardation of various organs including kidneys and lungs, which could be examined by microscopic analysis. It was suggested that abnormality of the somatic organs began to appear at the fetal stage in this case of xeroderma pigmentosu

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1978.tb01269.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY