摘要:

Histopathological relationship between orcein positive hepatocellular material (ophm) reported by SIPPONEN and human lipofuscin (hip) was investigated. Ophm was found to be very similar to hip of the liver as well as of heart muscle cell morphologically and histochemically, and it seems to be quite rational to consider that ophm may be a substance which belongs to the lysosome series comprising hip and prelipofuscin. Ophm has been discussed often from a view point of copper contents, which was considered to be one of its specific characters, but it has become clear that hip can also possess copper according to our Investigation and to some reports of other researchers. This fact throws a doubt on the significance of the specific relation between ophm and copper, as well as the usability of orcein stain for detecting copper in histological specimens.Victoria blue stain is quite useful for the detection of hip including ophm, since it gives a blue color for it with a good contrast against red colored cytoplasm and nucleus. However, this can not be considered as a method for specific stain of copper either.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb02153.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The author respectively examined the localization of hepatitis B surface antigen (HBsAg) in 150 autopsy cases of hepatocellular carcinoma (HCG) with particular attention to growth pattern of HCG. Varying numbers of orcein‐positive cells were observed In 42 cases (28.0%) with various distribution patterns, and all the 42 cases were confirmed with immunoperoxidase technique for HBsAg. HBsAg‐positive cells were detected in HCG tissue In 10 cases (6.6%) among the 150 cases. Regarding the growth pattern of HCG in the 10 cases, many HBsAg‐positive cells were seen In HGGs of the sinusoidal and replacing types, in which the hepatocytes were frequently retained in the cancerous tissue, particularly around the tumor‐nontumor boundary. On the other hand, there was no HBsAg‐positive cell in the encapsulated type HGC which was the most common (approximately 50%) in the present study and in which the retained hepatocytes were hardly seen. Meticulous histological observation after decoloration of the positive reaction to HBsAg in the sections treated with immunoperoxidase technique and subsequent eosin stain disclosed that HBsAg‐positive cells in HGC tissue were retained hepatocytes in 9 cases, and the possibility of HCC cell was not denied in one case. Moreover, HBsAg‐positive cells were never detected in tumor thrombi of the portal vein branches and pulmona

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb02154.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Intrahepatic biliary tree with either florid duct lesions or a moderate to severe degree of the duct loss in four livers with chronic hepatic diseases other than primary biliary cirrhosis were studied with histometric and serial section observations. Florid duct lesions, distributed segmentally in the liver, were found in one case with incomplete septal cirrhosis and one case with idiopathic portal hypertension. The florid duct lesions including marked plasma cell infiltration and occasional periductal granulomas, were not associated with any bile duct loss in the two cases. The duct lesions were reversible in one case during a long clinical course. On the other hand, a moderate to severe bile duct loss with biliary epithelial degeneration and necrosis was associated with no or little periductal inflammatory cell infiltration in one other case with chronic intrahepatic cholestasis, probably drug‐induced, and in one case with idiopathic portal hypertension. Although florid duct lesions and bile duct loss were important diagnostic features of primary biliary cirrhosis, one of them was observed to develop independently in severely diseased livers, not consistent with a diagnosis of primary biliary cirrhosis, sclerosing cholangitis or intrahepatic bile duct paucity syndrom

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb02155.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

In order to clarify the roles of hypertension and hypercholesterolemia in atherogenesis, the cerebral arterial endothelium of experimentally hypertensive female rats fed an atherogenic diet were investigated by scanning and transmission electron microscopy. The hypertension was induced by constriction of the bilateral renal arteries. In both hypertensive groups, group I (hypertension+hypercholesterolemia) and group III (hypertension), many endothelial cells covered with numerous microvillous projections were observed. Pinocytotic vesicles and caveolae were also increased in these endothelial cells. On the other hand, in group II loaded with only hypercholesterolemia, microvillous projections on the endothelial cells tended to be increased, but its grade seemed to be lower than those of the hypertensive groups. These findings suggest that hypertension appears to be more effective than hypercholesterolemia in the cause of the increase of microvilli on the endothelial cell surfaces and the increase may be related to increased permeability in the cerebral arteries.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb02156.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

In mice fed on an atherogenic diet for 4 to 8 months, the aortas were examined by transmission and scanning electron microscopy. After 8 months of being fed a high cholesterol diet, the animals developed aortic intimal lesions composed mainly of proliferated modified smooth muscle cells with an increase of connective tissues. Scanning electron microscopy showed changes in aortic luminal surface consisting primarily of altered distribition of microvilli. Quantitative analysis of these changes showed a statistically significant (P<0.001) increase in cholesterol‐fed animals compared with controls and a significant (P<0.001) difference between at 4 months and at 8 months in cholesterol‐fed mice. Ultrastructural study on the uptake of protein tracer, horseradish peroxidase (HRP), by aortic endothelial cellsin vitrowas performed. The uptake of HRP was essentially the same for controls and cholesterol‐fed mice at 4 months, but a statistically significant (P<0.005) increased uptake of HRP was observed at 8 months. Additional mice were subjected to nephrectomy with DOCA administration for 4 months along with a cholesterol‐feeding. These animals showed a 2‐fold increase in HRP‐uptake compared with nephrectomized mice without a cholesterol‐feeding. These results suggest that the enhanced pinocytotic activity of aortic endothelial cellsin vitro, especially in hypertensive condition, and altered distribution of microvilli might be correlated to the arterioscle

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb02157.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

When a normal rabbit is injected Intravenously with Evans blue, focal blue areas showing strong permeability appear in a region of the aortic arch and in a region distal to the orifice of the aortic branches. These blue areas consist of the dot stained area but are not homogeneous blue area.The morphological changes in the intima of the dot stained area and the relationship between the appearance of dot stained area and lipid deposition in the aorta of cholesterol fed rabbit were investigated. Evans blue showed focal acceleration of the permeability at the region of the aortic arch as early as 10 minutes. Light and electron microscopically such dot stained areas showed the widening of subendothelial space, lifting of some endothelial cells, intracytoplasmic vacuoles and membranous structure, junctional dilatation, and enhanced permeability of peroxidase. In 2% cholesterol fed rabbits, lipid deposition showed a punctuate pattern similar to the dot stained area of normal rabbit.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb02158.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

An electron microscopic immunohistochemical localization of thyroglobulin (TG) using PAP methods has been made in 15 cases of cold follicular adenoma. All cases of follicular adenoma showed organ specific functions such as synthesis, storage, reabsorption, and hydrolysis of thyroglobulin except for an area composed of follicular cells with trabecular arrangement. Immuno‐reaction product for TG was precisely demonstrated in follicular lumina, subapical vesicles and reabsorbed colloid droplets. The reaction product observed in the follicular lumen was clearly demarcated from the cytoplasm of the follicular cells by the apical plasma membrane. The subapical vesicles ranging approximately from 50 mμ to 300 mμ in diameter were rarely observed in follicular adenoma and some of them fused with the reabsorbed colloid droplets. The reabsorbed colloid droplets usually had the intense reaction product and hydrolyzed colloid droplets had a vacuole containing floccular low electron dense materials. There is no reaction product in rough endoplasmic reticulum and Golgi comple

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb02159.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Focal hyperplasia of intracytoplasmic filaments of 100Å in diameter was observed within chief cells of the parathyroid gland in dogs treated with nalidixic acid. The structure, as a rule, was located in the neighborhood of the nucleus and no other cell organelles were detected within it. Its size had a wide spectrum from a small part of the cytoplasm to as large as half a cell. Golgi apparatus and rough endoplasmic reticulum in the involved cells had a tendency to arophy. These cells occasionally contained less secretory granules. Lipid droplets are diffusely increased. The pathogenesis and significance of the present intracytoplasmic filaments remain to be determined. However, as lipid deposition, atrophy of Golgi apparatus, poorly developed rough endoplasmic reticulum occur in a hypofunctional or degenerative state, it might be possible that filamentous hyperplasia is closely associated with that state

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb02160.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Renal biopsies from 10 patients who had residual symptoms of toxemia of pregnancy were examined by light, electron and immunofluorescent microscopy. The biopsies were performed 1 month to 3 months postpartum. The glomeruli showed a similar lesion, even though to a lesser degree,to that found in toxemia of pregnancy. Subepithelial swellings and endothelial cytopasmic microtubular structures were also observed. Mesangial and monocyte‐like cells contained lysosomes,cholesterol clefts and lipid‐containing vacuoles with or without myelin figures, suggesting reparative process in the glomeruli. The present study indicates that the glomerular injury persists for a few months after delivery, together with the concurrent reparative proc

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb02161.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Following light microscopic survey of the incidence of elastic tissue in 80 salivary gland tumors, tissue samples from 14 pleomorphic adenomas, three myoepitheliomas, and eight adenoid cystic carcinomas were processed for cytochemical demonstration of elastin with the tannic add stain for ultrathin sections. For comparative study, some other tumor types devoid of elastic tissue at the light microscopic level and non‐neoplastic submandibular glands were also investigated. Elastic deposits of varying amounts were clearly revealed on the basal‐lamina‐like material and/or masses of microfibrils in the matrix close to the neoplastic myoepithelium and, to a lesser degree, immediately beneath the non‐neoplastic myoepithelium. None of the other tumor types without myoepithelial differentiation contained elastic deposits closely associated with the neoplastic cells. Intimate topographical relationship of such an immature elastic fiber or developing elastic tissue to the neoplastic myoepithelial cells strongly indicated the primary origin of elastic components from these cells. It is postulated that the potential of salivarytumorcells to produce elastin is regarded as an indicator of their myoepithelial nature or differen

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb02162.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY