摘要:

We demonstrated the distribution of asbestos bodies (ABs) in the lungs of 656 consecutive autopsy cases by KOH digestion method. Nine patients including one case with asbestosis had more than a thousand ABs. These patients were all blue‐collar workers except the two cases without occupational history. The tissue sections for these patients revealed the constant presence of more than one or two ABs. In addition, five of these subjects had parenchymal and/or peribronchiolar fibrosis. On the basis of the result of AB counts, we selected 17 subjects for enumeration and typing of asbestos fibers (AFs). The number of AFs roughly correlated with that of ABs. However, the ratio of AFs to ABs ranged from 13 to 3,700. The fiber types in our selected subjects were almost always amphibole (crocidolite, amosite). These findings indicate that modest or secondary exposure to asbestos causes virtual asbestosis to several subjects among the general populatio

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb02242.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

A specific bile duct injury is frequently observed in the liver following bone marrow transplantation. The materials used for study consisted of liver specimens obtained from 7 autopsy cases and 2 biopsies. The pathological lesions were analyzed by reconstruction of serial sections and by immunohistochemical examination. Injury following bone marrow transplantation occurred most frequently in bile ducts right after the canalicular‐ductular junction to ducts having an outer diameter of 50–60 μ, being especially prominent in those less than 30 μ in diameter. They preserved their continuity and there was neither complete destruction nor loss of bile ducts as seen in primary biliary cirrhosis. Immunohistochemical examination showed predominancy of suppressor T lymphocytes around ducts and they were frequently found adjacent to epithelial cells of bile ducts. C3components and HLA‐DR antigens were confirmed to be localized in ductular epithelia in the area where injury took place. Although their localization and site of injury did not necessarily coincide, the participation of an immunologic mechanism against injury cannot be denied. Evidence of a relation between ductular injury and cytomegalovirus infection could not be o

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb02243.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

A study of double immunofluorescence staining of immunoglobulins and sialic acids in the glomeruli from patients with membranous nephropathy was described. Renal biopsy specimens from patients with membranous nephropathy were stained with rhodamine‐labeled limulus polyphemus (LPA) and tricum vulgaris (WGA). The binding of LPA or WGA was hardly observed in the glomerular capillary walls from patients with membranous nephropathy although the deposition of immunoglobulin was marked. Both binding of LPA and deposition of IgG in the glomerular capillary walls were observed in only one out of nine patients with membranous nephropathy (11%). In that case, binding of LPA was observed in the inside of glomerular capillary walls although the deposition of IgG was observed on the outside of the walls. It was suggested that decrease of sialic acids in the glomerular capillary walls might be due to deposition of IgG in some patients with membranous nephropath

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb02244.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

In close inspection of 88 total cystectomy specimens, 20 were found to have rheumatoid nodule‐like lesions known as “focal palisading granulomas”. Nineteen of these nodules were from patients with a history of previous transurethral resection (TUR). Such granulomas were identified in the prostate in 3, in the bladder in 13, and in both prostate and bladder in another 3. The remaining one patient had undergone a cold cup biopsy followed by electric coagulation. The granulomas were characterized microscopically by the presence of central necrosis surrounded by palisading histiocytes and fibroblasts, and were situated in areas of the previous TUR. There were no granulomas in 20 other patients with no history of previous TUR and other surgical procedures. The lesions were commonly seen in the superficial zone of the bladder wall and in the prostate and, as proved by serial sections, opened onto the mucosal surface of the bladder and urethra, respectively. The observation confirmed that the focal palisading granulomas occurred not only in the prostate but also in the bladder, and seemed to be closely related to surgical procedures, especially to the electrocauterizing process, in both the bladder and pro

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb02245.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

We report the ultrastructural findings in rectal and skin biopsies in four cases with adult GM1‐gangliosidosis. The rectal specimens taken under endoscopic control contained submucosal plexuses, which were easily identified on light‐microscopic and electron‐microscopic examination. In all cases the ganglion cells in submucosal plexus contained characteristic osmiophilic lamellar Inclusions, some of which were typical membranous cytoplasmic bodies, but others showed pleomorphism in shape and diameter. Lipofuscin bodies were commonly observed in the ganglion cells, Schwann's cells, vascular endothelial cells, and perithelial cells in rectal biopsy, and membrane‐bound clear vacuoles were occasionally seen in the cytoplasm of rectal and cutaneous fibroblasts. However, the axons of unmyelinated nerves in both biopsies showed a normal appearance, and there was no significant change in the eccrine glands in skin biopsy. Comparing rectal and skin biopsies, the former was found to be more valuable for morphological diagnosis of this disease, and our ultrastructural examination of rectal biopsy revealed that enteric nerve cells were involved even in adult GM1‐gangliosidosis with a benign course and restricted cerebra

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb02246.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

To estimate microfilament bundles in the endothelial cells of flow loaded arteries quantitatively, blood flow change was produced in the common carotid arteries of eleven female beagle dogs (1–2 years of age, 8–10 kg) employing the arteriovenous shunt method between the common carotid artery and external jugular vein. After 1 week, the amount of microfilament bundles was measured with a point counting method on transmission electron microscopical photographs. In the arteries loaded by highly elevated blood flow, the average thickness of microfilament bundles increased over 0.1 μm (non‐operative control: 0.01–0.02 μ) and the average longitudinal cut area of a microfilament bundle grew over 0.7 μm2(non‐operative control: 0.2 μm2). The number of bundles increased over 1.0 piece (non‐operative control: 0.2 pieces) per unit length (μm) in transverse section and increased over 0.5 pieces (non‐operative control: 0.2 pieces) in longitudinal section. The average thickness of the bundles significantly correlated to the blood flow increase ratio (the ratio of the blood flow rate of the final measurement to that before anastomosis). We consider that the microfilament bundles are the structure developed to combat the wall shear stress induced by the e

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb02247.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Hepatic vascular changes are generally associated to fibrotic lesions of the liver but their role in the development and extension of fibrosis is not well known. By performing a portacaval anastomosis in rats, a proliferation of hepatic vessels was attained progressively, comparable to proliferations seen in cirrhosis and schistosomiasis. Such a modification of the hepatic vasculature was accompanied by changes in the connective matrix of the liver, mainly related to the vascular walls. Moreover, sinusoidal capillarization was detected at the electron microscopical level whereas no alterations could be seen neither in the distribution nor in the quality of the connective matrix proteins with Immunofluorescence technique. The only modification detected by light microscopy, at this zone, was the shrinked aspect of reticulin fibers which could be related to the important liver atrophy provoked by the anastomosis. It can be assumed that in liver pathology, selective changes occur in the connective matrix components depending on the type of injury. Necrotic and inflammatory processes lead mainly to the deposition of interstitial collagens and associated proteins, related to a diffuse stroma reaction resultant from activation of specific cell populations whereas in portacaval anastomosis, a selective increase of vascular connective matrix (mainly basement membrane components) occurs as a direct consequence of vessel proliferation.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb02248.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Serous adenocarcinoma of the ovary with estrogen and alpha‐fetoprotein (AFP) production was serially heterotransplanted into nude mice. The original tumor presented marked luteinization of stromal cells with elevated urine levels of estrogen before the operation. Estrogen levels reduced to the normal level shortly after the operation. Histologic features of the original tumor were basically retaind in the transplanted tumor, but no luteinization of stromal cells was observed and serum estrogen levels in nude mice were elevated. Immunohlstochemically, AFP was found to be positive in some tumor cells of transplanted tumor, but serum AFP levels were not elevate

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb02249.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Variable numbers of foamy cells (macrophages with foamy cytoplasm) were noted in generalized organs from four patients who received repeated platelet transfusions. The underlying disease in three cases was aplastic anemia, and the remaining case was chronic myelocytic leukemia. In two patients (aplastic anemia and chronic myelocytic leukemia) bone marrow transplantation (BMT) was done. Opportunistic infection was noted in three out of four cases. The foamy cells were stained black with Sudan black B. Variable amounts of materials immunoreactive with antihuman platelet antibody were demonstrated in most of the foamy cells. Ultrastructurally, the foamy cells contained myelin‐like materials. The foamy cells described here resembled those demonstrable in the spleen from patients with idiopathic thrombocytopenic purpura. We suggest that the foamy appearance of the macrophage results from incomplete intracellular degradation of phagocytosed platelet

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb02250.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The distribution of thorium dioxide (Thorotrast) in the liver from a Thorotrast‐administered autopsy case was examined stereographically by analytical scanning electron microscopy. Thorium particles were detected in the macrophages of the portal triad and hepatic sinusoid. These macrophages were irregularly shaped and tended to be aggregated. In the sinusoid, accumulation of the macrophages formed a thrombus‐like structure. Furthermore, observed in the sinusoid were free Thorotrast particles that appeared to have been released into the sinusoid as a result of breakdown of the macrophages (Kupffer cel

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb02251.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY