摘要:

Quantitative and qualitative changes incollagen synthetic activity by rabbit arterial smooth muscle cells were monitored during spontaneous phenotypic modulation from days 2‐15 of culture. The cultured smooth muscle cells transformed into a synthetic phenotype, reaching a maximum of 94.6% on day 4, and then gradually returned to a conractile phenotype accounting for 59.3% on day 15 of culture. The maximum collagen synthesis was found on day 7 when the cells were in early quiescent phase and showed a 91.7% synthetic phenotype. With an increasing proportion of cells decreased in parallel with the reduction in total protein synthesis. Synthesis of type I collagen was predominant, and the proportion of type I+III collagen was over 85% during the entire period of culture. Synthetic activity of type IV collagen, however, was relatively increased, and reached 3.8±0.4% a t day 15 in comparison with 0.81±0.1% in the late logarithmic growth phase on day 4. This significant increment of type IV collagen in vitro seems to be correlated with the phenotypic modulation of cultured smooth muscle cells into a contractile phenotype. Acta Pathol Jpn 40: 157‐164,

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb03317.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

The effect of epidermal growth factor (EGF) on rat stomach carcinogenesis induced by N‐methyl‐N′‐nitro‐N‐nitro‐soguanidine (MNNG) was studied. Male Wistar rats given MNNG for 30 weeks in drinking water (80 μg/ml) were treated with S.C. injections of human EGF (10 μg/kg, once daily) at various stages of the carcinogenesis. Four (30.8%) out of 13 rats treated with EGF immediately after cessation of the MNNG treatment had stomach tumors including one adenocarcinoma, one adenoma and two carcinoids. No stomach tumor was found in rats treated with MNNG alone or in those treated with MNNG and EGF for different periods such as synchronously for 10 weeks, for 30 weeks or throughout the experiment. These findings suggest a possible enhancing effect of EGF on stomach carcinogenesis in rats. Acta Pathol Jpn 40

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb03318.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Changes in glomerular anionic sites were analyzed in 31 patients with various types of glomerulonephritis using the high iron diamine thiocarbohydrazide‐silver proteinate method. On the basis of the degree of proteinuria at the time of biopsy, the patients were divided into 3 groups as follows: Group 1, less than 10 mg/kg/24 h; Group 2, 10‐50 mg/kg/24 h; Group 3, more than 50 mg/kg/24 h. The number of glomerular anionic sites per 300 nm length of the lamina rara externa was 16.20±3.37 in Group 1, 12.19±3.42 in Group 2 and 9.97±2.51 in Group 3. Moreover, smaller and irregularly distributed anionic sites, and a greater loss of anionic sites in the paramesangial region were observed in Groups 2 and 3. On the other hand, there was no significant correlation between the mesangial sclerosis index and the number of anionic sites (r =−0.40) in patients with IgA nephropathy (9 cases) and nephritis of Henoch‐Schönlein purpura (6 cases). These results suggest that the proteinuria seen in various types of glomerulonephritis is related to the loss of glomerular anionic sites, i.e., dysfunction of the charge‐selective barrier. Acta Pathol Jpn 40:

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb03319.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

The effects of monensin on subcellular structure, release of thyroglobulin (TG) and peroxidase (PO) activity were investigated using primary cultures of thyroid cells obtained from patients with Basedow's disease (Basedow's cells). TG concentration in the culture medium was measured by a sandwich enzyme immunoassay and the amount of TG in cultured cells was measured with an identical sandwich enzyme immunoassay after lysis of the cells with Triton X‐100. PO activity of cultured cells was measured by a biochemical method. Addition of TSH (10 mU/ml/day) to the culture medium increased the synthesis and release of TG. When monensin (1 μm/l) was added to the medium on the last day of a 3‐day incubation with TSH, the Golgi complex showed vacuolative change ultrastructurally, and the amount of intracellular TG was increased, whereas the amount of TG in the culture medium and PO activity became lower than those in the control group. These results suggest that in cultured Basedow's cells, TG is secreted through the Golgi complex, and that the activity of PO is elevated after processing in the Golgi complex. Acta Pathol Jpn 40: 181‐186

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb03320.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Parafollicular cells (C‐cells) in benign and malignant thyroid lesions were studied immunohistochemically with a polyclonal anti‐calcitonin (CT) antibody. The C‐cells were seen most frequently in the middle third of the lateral lobes in the thyroid gland of normal individuals and patients with Graves' disease and chronic thyroiditis, although in the latter the number of such cells was significantly decreased (p<0.05). In adenomatous goiter, C‐cells were present in nodular lesions from an early stage of nodule development (frequency about 19%), whereas in the later stage these cells were rarely observed inside type 1 nodules, which were generally characterized by an admixture of follicles with considerably different sizes. However, C‐cells were not observed inside type 2 nodules, which were composed of similar‐sized follicles, or in the parenchyma of 56 cases of benign and malignant thyroid tumors. These findings suggest that since C‐cells are present in nodular lesions, the histogenesis of adenomatous goiter is quite different from that of follicular adenoma; thyroid neoplasms generally contain no C‐cells in the parenchyma. Acta Pathol Jpn 40

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb03321.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

We present four cases of an unusual diffuse sclerosing variant of papillary carcinoma of the thyroid occurring in Japanese women ranging in age from 17 to 42 years. Macroscopically, both lobes of the thyroid were involved, being diffusely enlarged and firm. Histologically, the tumor was characterized by squamous metaplasia of tumor cells, numerous psammoma bodies, extensive stromal fibrosis. severe lvmphocvtic infiltration with formation of lymph follicles, and thin bundles of smooth muscle cells in the fibrous stroma. The tumor islands were located mainly within the dilated lymphatic vessels and the metastatic tumor involved the bilateral cervical lymph nodes in all cases. It is assumed that papillary carcinoma of this type invades the thyroid lymphatics during the early stage and, without forming nodular lesions, disseminates to both lobes with extensive metastasis to the cervical lymph nodes. It should be noted that papillary carcinoma of this type often suggests chronic thyroiditis clinically because of the symmetrical enlargement of the thyroid and frequent positive anti‐thyroid antibodies in the serum. Some workers have reported the prognosis to be unfavorable because of extensive lymphatic involvement, but the prognosis of this variant has not yet been defined. Acta Pathol Jpn 40: 193‐198, 1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb03322.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Twelve cases of alveolar soft part sarcoma (ASPS) were reviewed. Seven of them arose primarily in the lower extremities, three i n the head and neck region, and two in other parts. ASPSs in the head and neck region occurred in children before 10 years of age, whereas ASPSs in the other regions occurred in rather older patients. Moreover, ASPSs of the head and neck were relatively small in size, and were diagnosed earlier than those in other regions. Histologically, six cases (including all the head and neck cases) contained considerable area of small and indistinct alveolar structures. Four cases showed remarkable cellular pleomorphism. lmmunohistochemical demonstration of vimentin, desmin, the β‐subunit of enolase and the MM isozyme of creatine kinase, together with the absence of immunoreactive cytokeratin, supported the myogenic nature of this rare tumor. A small number of S‐100 protein‐positive tumor cells were also observed. Followup data for these cases disclosed that the tumors containing considerable area of small alveoli and uniform small tumor cells formed distant metastases at an early stage. Acta Pathol Jpn 40: 199‐2

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb03323.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A peculiar case of astrocytoma showing a histological appearance similar to granular cell tumor was reported. The tumor consisted of large round cells containing numerous intracytoplasmic eosinophilic granules, partially intermingled with atypical astrocytes, and part of it showed a transition to distinct areas of fibrillary astrocytoma. The granules were periodic acid‐Schiff‐positive (with resistance to diastase digestion), negative for fat stains and revealed lectin‐binding patterns similar to those in granular cell tumor. Ultrastructurally the granules were partially membrane‐bound, dense bodies compatible with secondary lysosomes. It was suggested that the granular cells were of astrocytic origin because of their immuno reactivity for glial fibrillary acidic protein (GFAP) and ultrastructural observation of intermediate filaments corresponding to GFAP. Acta Pathol Jpn 40: 206‐

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb03324.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

An autopsy case of adult hepatoblastoma is presented. The patient was an 8 2 year‐old male with chronic hepatitis of 7 years' duration. The liver tumor was detected 6 months before death. Autopsy revealed a large hepatic tumor occupying about 80% of the entire liver. Histologically, the tumor showed typical features of mixed epithelial and mesenchymal type hepatoblastoma. The epithelial component consisted of fetal and embryonal cell types. The mesenchymal component showed primitive spindle‐shaped cells with various degrees of cellularity. Chondroid areas and a few foci of osteoid formation were also present. Acta Pathol Jpn 40: 212‐218,

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb03325.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

An autopsy case of malignant schwannoma arising in the intracranial trigeminal nerve is reported. The tumor involved the right cerebellopontine angle of the brain stem in an 18‐year‐old man. The spindle‐shaped tumor cells with eosinophilic cytoplasm proliferated in fascicles and exhibited hypercellularity, pleomorphisrn, increased mitotic activity and invasive growth. Ultrastructurally, inter‐digitating cytoplasmic processes and a few fragmented basal lamina‐like structures were observed. Immunohistochemically, some tumor cells were reactive with conventional anti‐S‐100 protein antibody, but negative for β subunit. Most tumor cells were positive for LY subunit of S‐100 protein. This is the eighth reported case of malignant schwannoma arising in the intracranial trigeminal nerve. Acta Pathol Jpn 4

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb03326.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY